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Product Info Summary
| SKU: | M09779 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-BBOX1 Mouse Monoclonal Antibody [Clone ID: OTI4F9]
SKU/Catalog Number
M09779
Size
100 µl
Description
Boster Bio BBOX1 mouse monoclonal antibody, clone OTI4F9 (formerly 4F9). Catalog# M09779. Tested in IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-BBOX1 Mouse Monoclonal Antibody [Clone ID: OTI4F9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09779)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4F9
Isotype
IgG1
Immunogen
Full length human recombinant protein of human BBOX1 (NP_003977) produced in HEK293T cell.
Reactive Species
M09779 is reactive to BBOX1 in Human, Mouse, Rat
Calculated molecular weight
44.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09779 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:4000
IHC 1:150
IF 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-BBOX1 mouse monoclonal antibody (M09779) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BBOX1.
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Immunofluorescent staining of HeLa cells using anti-BBOX1 mouse monoclonal antibody (M09779).
Specific Publications For Anti-BBOX1 Mouse Monoclonal Antibody [Clone ID: OTI4F9] (M09779)
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