Product Info Summary
SKU: | A03281-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-CD48 Antibody Picoband™
View all CD48/SLAMF2 Antibodies
SKU/Catalog Number
A03281-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD48 Antibody Picoband™ catalog # A03281-1. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD48 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03281-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD48 recombinant protein (Position: E51-D146). Human CD48 shares 54.7% and 49% amino acid (aa) sequence identity with mouse and rat CD48, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03281-1 is reactive to CD48 in Human
Applications
A03281-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
27.683kDa
Background of CD48/SLAMF2
CD48 antigen (Cluster of Differentiation 48), also known as B-lymphocyte activation marker (BLAST-1) or signaling lymphocytic activation molecule 2 (SLAMF2), is a protein that in humans is encoded by the CD48 gene. This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD48 using anti-CD48 antibody (A03281-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: CEM whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD48 antigen affinity purified polyclonal antibody (Catalog # A03281-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD48 at approximately 39KD. The expected band size for CD48 is at 28KD.
Protein Target Info & Infographic
Gene/Protein Information For CD48 (Source: Uniprot.org, NCBI)
Gene Name
CD48
Full Name
CD48 antigen
Weight
27.683kDa
Alternative Names
BCM1 surface antigen; BCM1; BCM1Leukocyte antigen MEM-102; BLAST; BLAST1; BLAST1TCT.1; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); CD48 antigen; CD48 molecule; CD48; hCD48; mCD48; MEM-102; SLAMF2 CD48 BCM1, BLAST, BLAST1, MEM-102, SLAMF2, hCD48, mCD48 CD48 molecule CD48 antigen|B-lymphocyte activation marker BLAST-1|BCM1 surface antigen|CD48 antigen (B-cell membrane protein)|SLAM family member 2|TCT.1|leukocyte antigen MEM-102|signaling lymphocytic activation molecule 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD48, check out the CD48 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD48: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD48 Antibody Picoband™ (A03281-1)
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No publications found for A03281-1
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CD48 Antibody Picoband™
Question
I was wanting to use your anti-CD48 antibody for WB for human b-cell pancreas on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human b-cell pancreas identification?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
You can see on the product datasheet, A03281-1 anti-CD48 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human b-cell pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-02
Question
Is this A03281-1 anti-CD48 antibody reactive to the isotypes of CD48?
B. Jackson
Verified customer
Asked: 2019-06-28
Answer
The immunogen of A03281-1 anti-CD48 antibody is E.coli-derived human CD48 recombinant protein (Position: E51-D146). Human CD48 shares 54.7% and 49% amino acid (aa) sequence identity with mouse and rat CD48, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-28
Question
Would anti-CD48 antibody A03281-1 work on canine WB with b-cell pancreas?
F. Anderson
Verified customer
Asked: 2019-03-04
Answer
Our lab technicians have not tested anti-CD48 antibody A03281-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CD48 antibody A03281-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine b-cell pancreas in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-04
Question
We are currently using anti-CD48 antibody A03281-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
A. Parker
Verified customer
Asked: 2018-01-29
Answer
The anti-CD48 antibody (A03281-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-29
Question
We are interested in to test anti-CD48 antibody A03281-1 on human b-cell pancreas for research purposes, then I may be interested in using anti-CD48 antibody A03281-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Krishna
Verified customer
Asked: 2016-11-07
Answer
The products we sell, including anti-CD48 antibody A03281-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-11-07
Question
Is there a BSA free version of anti-CD48 antibody A03281-1 available?
M. Jones
Verified customer
Asked: 2013-07-09
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD48 antibody A03281-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-07-09