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Product Info Summary
| SKU: | PB9999 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-BCA1/CXCL13 Antibody Picoband®
SKU/Catalog Number
PB9999
PB1043 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BCA1/CXCL13 Antibody Picoband® catalog # PB9999. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BCA1/CXCL13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9999)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109). Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9999 is reactive to Cxcl13 in Mouse
Observed Molecular Weight
11 kDa
Calculated molecular weight
11.9 kDa
Background of Cxcl13
C-X-C motif chemokine 13 (CXCL13), also known as B cell-attracting chemokine 1 (BCA-1), is a protein that in humans is encoded by the CXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family. Its gene is mapped to human chromosome 4 in a cluster of other CXC chemokines. This protein has contained 109 amino acids. It is expressed highly in the liver, spleen, lymph nodes and gut of humans. The protein induces chemotaxis of BLR1-expressing B cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9999 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: recombinant mouse BCA1/CXCL13 protein
IHC: mouse spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of BCA1/CXCL13 using anti-BCA1/CXCL13 antibody (PB9999).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours.
Lane 1: recombinant mouse BCA1/CXCL13 protein 10ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCA1/CXCL13 antigen affinity purified polyclonal antibody (PB9999) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCA1/CXCL13 at approximately 11 kDa.
Click image to see more details
IHC analysis of BCA1/CXCL13 using anti-BCA1/CXCL13 antibody (PB9999).
BCA1/CXCL13 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCA1/CXCL13 Antibody (PB9999) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-BCA1/CXCL13 Antibody Picoband® (PB9999)
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Customer Q&As
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17 Customer Q&As for Anti-BCA1/CXCL13 Antibody Picoband®
Question
Is PB9999 specific to mice and has no cross-reaction with human cells?
Verified customer
Asked: 2022-04-22
Answer
Our lab hasn't got any data to indicate the antibody has no cross-ractivity with human cells. According to the immunogen sequence of Anti-BCA1/CXCL13 Antibody Picoband™ (PB9999), mouse BCA1/CXCL13 shares very low homology with human BCA1/CXCL13. "E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109). Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1." We suggest running a pilot test.
Boster Scientific Support
Answered: 2022-04-23
Question
Our lab used your anti-BCA1/CXCL13 antibody for WB on skeletal muscle in the past. I am using mouse, and I plan to use the antibody for IHC next. We are interested in examining skeletal muscle as well as peripheral blood leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
I have checked the website and datasheets of our anti-BCA1/CXCL13 antibody and I see that PB9999 has been tested on mouse in both WB and IHC. Thus PB9999 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-21
Question
See below the WB image, lot number and protocol we used for peripheral blood using anti-BCA1/CXCL13 antibody PB9999. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-04
Question
We are interested in to test anti-BCA1/CXCL13 antibody PB9999 on mouse peripheral blood for research purposes, then I may be interested in using anti-BCA1/CXCL13 antibody PB9999 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
The products we sell, including anti-BCA1/CXCL13 antibody PB9999, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-BCA1/CXCL13 antibody PB9999. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-06
Question
Will PB9999 anti-BCA1/CXCL13 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
You can see on the product datasheet, PB9999 anti-BCA1/CXCL13 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-09
Question
My boss were satisfied with the WB result of your anti-BCA1/CXCL13 antibody. However we have observed positive staining in tonsil secreted. using this antibody. Is that expected? Could you tell me where is CXCL13 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
According to literature, tonsil does express CXCL13. Generally CXCL13 expresses in secreted. Regarding which tissues have CXCL13 expression, here are a few articles citing expression in various tissues:
Peripheral blood leukocyte, Pubmed ID: 9463416
Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-24
Question
Is a blocking peptide available for product anti-BCA1/CXCL13 antibody (PB9999)?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
We do provide the blocking peptide for product anti-BCA1/CXCL13 antibody (PB9999). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-29
Question
Will anti-BCA1/CXCL13 antibody PB9999 work on bovine IHC with tonsil?
E. Krishna
Verified customer
Asked: 2019-06-14
Answer
Our lab technicians have not validated anti-BCA1/CXCL13 antibody PB9999 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-BCA1/CXCL13 antibody PB9999 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine tonsil in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-14
Question
Will anti-BCA1/CXCL13 antibody PB9999 work for IHC with peripheral blood?
S. Mitchell
Verified customer
Asked: 2019-03-27
Answer
According to the expression profile of peripheral blood, CXCL13 is highly expressed in peripheral blood. So, it is likely that anti-BCA1/CXCL13 antibody PB9999 will work for IHC with peripheral blood.
Boster Scientific Support
Answered: 2019-03-27
Question
We are currently using anti-BCA1/CXCL13 antibody PB9999 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on feline tissues as well?
A. Wu
Verified customer
Asked: 2018-07-05
Answer
The anti-BCA1/CXCL13 antibody (PB9999) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-05
Question
I was wanting to use your anti-BCA1/CXCL13 antibody for IHC for mouse peripheral blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse peripheral blood identification?
Z. Huang
Verified customer
Asked: 2018-02-22
Answer
As indicated on the product datasheet, PB9999 anti-BCA1/CXCL13 antibody has been tested for ELISA, IHC, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse peripheral blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-22
Question
My question regarding product PB9999, anti-BCA1/CXCL13 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9999 anti-BCA1/CXCL13 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-15
Question
We have seen staining in mouse skeletal muscle. Any tips? Is anti-BCA1/CXCL13 antibody supposed to stain skeletal muscle positively?
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
Based on literature skeletal muscle does express CXCL13. Based on Uniprot.org, CXCL13 is expressed in tonsil, peripheral blood leukocyte, peripheral blood, skeletal muscle, among other tissues. Regarding which tissues have CXCL13 expression, here are a few articles citing expression in various tissues:
Peripheral blood leukocyte, Pubmed ID: 9463416
Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-10-16
Question
Is there a BSA free version of anti-BCA1/CXCL13 antibody PB9999 available?
H. Jackson
Verified customer
Asked: 2017-02-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-BCA1/CXCL13 antibody PB9999 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-02-14
Question
I see that the anti-BCA1/CXCL13 antibody PB9999 works with IHC, what is the protocol used to produce the result images on the product page?
M. Collins
Verified customer
Asked: 2016-04-20
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-04-20
Question
Is this PB9999 anti-BCA1/CXCL13 antibody reactive to the isotypes of CXCL13?
E. Banerjee
Verified customer
Asked: 2014-08-27
Answer
The immunogen of PB9999 anti-BCA1/CXCL13 antibody is E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109). Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-08-27
