Anti-BCA1/CXCL13 Antibody Picoband™

CXCL13/BLC/BCA-1 antibody

Boster Bio Anti-BCA1/CXCL13 Antibody Picoband™ catalog # PB9999. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse.

Product Info Summary

SKU: PB9999
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-BCA1/CXCL13 Antibody Picoband™

View all CXCL13/BLC/BCA-1 Antibodies

SKU/Catalog Number

PB9999

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BCA1/CXCL13 Antibody Picoband™ catalog # PB9999. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BCA1/CXCL13 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9999)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109). Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9999 is reactive to CXCL13 in Mouse

Applications

PB9999 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

13 kDa

Calculated molecular weight

12.664kDa

Background of CXCL13/BLC/BCA-1

C-X-C motif chemokine 13 (CXCL13), also known as B cell-attracting chemokine 1 (BCA-1), is a protein that in humans is encoded by the CXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family. Its gene is mapped to human chromosome 4 in a cluster of other CXC chemokines. This protein has contained 109 amino acids. It is expressed highly in the liver, spleen, lymph nodes and gut of humans. The protein induces chemotaxis of BLR1-expressing B cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For CXCL13 (Source: Uniprot.org, NCBI)

Gene Name

CXCL13

Full Name

C-X-C motif chemokine 13

Weight

12.664kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant) CXCL13 ANGIE, ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13 C-X-C motif chemokine ligand 13 C-X-C motif chemokine 13|B-cell chemoattractant|B-cell-attracting chemokine 1|B-cell-homing chemokine (ligand for Burkitts lymphoma receptor-1)|B-lymphocyte chemoattractant|CXC chemokine BLC|b cell-attracting chemokine 1|b lymphocyte chemoattractant|chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)|small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)|small-inducible cytokine B13

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CXCL13, check out the CXCL13 Infographic

CXCL13 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CXCL13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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17 Customer Q&As for Anti-BCA1/CXCL13 Antibody Picoband™

Question

Is PB9999 specific to mice and has no cross-reaction with human cells?

Verified customer

Asked: 2022-04-22

Answer

Our lab hasn't got any data to indicate the antibody has no cross-ractivity with human cells. According to the immunogen sequence of Anti-BCA1/CXCL13 Antibody Picoband™ (PB9999), mouse BCA1/CXCL13 shares very low homology with human BCA1/CXCL13. "E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109). Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1." We suggest running a pilot test.

Boster Scientific Support

Answered: 2022-04-23

Question

Our lab used your anti-BCA1/CXCL13 antibody for WB on skeletal muscle in the past. I am using mouse, and I plan to use the antibody for IHC next. We are interested in examining skeletal muscle as well as peripheral blood leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

I have checked the website and datasheets of our anti-BCA1/CXCL13 antibody and I see that PB9999 has been tested on mouse in both WB and IHC. Thus PB9999 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-21

Question

See below the WB image, lot number and protocol we used for peripheral blood using anti-BCA1/CXCL13 antibody PB9999. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-04

Question

We are interested in to test anti-BCA1/CXCL13 antibody PB9999 on mouse peripheral blood for research purposes, then I may be interested in using anti-BCA1/CXCL13 antibody PB9999 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

The products we sell, including anti-BCA1/CXCL13 antibody PB9999, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-19

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-BCA1/CXCL13 antibody PB9999. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-06

Question

Will PB9999 anti-BCA1/CXCL13 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

You can see on the product datasheet, PB9999 anti-BCA1/CXCL13 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-09

Question

My boss were satisfied with the WB result of your anti-BCA1/CXCL13 antibody. However we have observed positive staining in tonsil secreted. using this antibody. Is that expected? Could you tell me where is CXCL13 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

According to literature, tonsil does express CXCL13. Generally CXCL13 expresses in secreted. Regarding which tissues have CXCL13 expression, here are a few articles citing expression in various tissues:
Peripheral blood leukocyte, Pubmed ID: 9463416
Skeletal muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-09-24

Question

Is a blocking peptide available for product anti-BCA1/CXCL13 antibody (PB9999)?

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

We do provide the blocking peptide for product anti-BCA1/CXCL13 antibody (PB9999). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-29

Question

Will anti-BCA1/CXCL13 antibody PB9999 work on bovine IHC with tonsil?

E. Krishna

Verified customer

Asked: 2019-06-14

Answer

Our lab technicians have not validated anti-BCA1/CXCL13 antibody PB9999 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-BCA1/CXCL13 antibody PB9999 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine tonsil in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-14

Question

Will anti-BCA1/CXCL13 antibody PB9999 work for IHC with peripheral blood?

S. Mitchell

Verified customer

Asked: 2019-03-27

Answer

According to the expression profile of peripheral blood, CXCL13 is highly expressed in peripheral blood. So, it is likely that anti-BCA1/CXCL13 antibody PB9999 will work for IHC with peripheral blood.

Boster Scientific Support

Answered: 2019-03-27

Question

We are currently using anti-BCA1/CXCL13 antibody PB9999 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on feline tissues as well?

A. Wu

Verified customer

Asked: 2018-07-05

Answer

The anti-BCA1/CXCL13 antibody (PB9999) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-05

Question

I was wanting to use your anti-BCA1/CXCL13 antibody for IHC for mouse peripheral blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse peripheral blood identification?

Z. Huang

Verified customer

Asked: 2018-02-22

Answer

As indicated on the product datasheet, PB9999 anti-BCA1/CXCL13 antibody has been tested for ELISA, IHC, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse peripheral blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-02-22

Question

My question regarding product PB9999, anti-BCA1/CXCL13 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-12-15

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9999 anti-BCA1/CXCL13 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-12-15

Question

We have seen staining in mouse skeletal muscle. Any tips? Is anti-BCA1/CXCL13 antibody supposed to stain skeletal muscle positively?

Verified Customer

Verified customer

Asked: 2017-10-16

Answer

Based on literature skeletal muscle does express CXCL13. Based on Uniprot.org, CXCL13 is expressed in tonsil, peripheral blood leukocyte, peripheral blood, skeletal muscle, among other tissues. Regarding which tissues have CXCL13 expression, here are a few articles citing expression in various tissues:
Peripheral blood leukocyte, Pubmed ID: 9463416
Skeletal muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-10-16

Question

Is there a BSA free version of anti-BCA1/CXCL13 antibody PB9999 available?

H. Jackson

Verified customer

Asked: 2017-02-14

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-BCA1/CXCL13 antibody PB9999 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-02-14

Question

I see that the anti-BCA1/CXCL13 antibody PB9999 works with IHC, what is the protocol used to produce the result images on the product page?

M. Collins

Verified customer

Asked: 2016-04-20

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-04-20

Question

Is this PB9999 anti-BCA1/CXCL13 antibody reactive to the isotypes of CXCL13?

E. Banerjee

Verified customer

Asked: 2014-08-27

Answer

The immunogen of PB9999 anti-BCA1/CXCL13 antibody is E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109). Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-08-27

Order DetailsPrice
PB9999

100μg

$370
PB9999-10ug

10μg sample (liquid)

$99
PB9999-Biotin

100 μg Biotin conjugated

$570
PB9999-Cy3

100 μg Cy3 conjugated

$570
PB9999-Dylight488

100 μg Dylight488 conjugated

$570
PB9999-Dylight550

100 μg Dylight550 conjugated

$570
PB9999-Dylight594

100 μg Dylight594 conjugated

$570
PB9999-FITC

100 μg FITC conjugated

$570
PB9999-HRP

100 μg HRP conjugated

$570
PB9999-APC

100 μg APC conjugated

$670
PB9999-PE

100 μg PE conjugated

$670
PB9999-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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