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- Table of Contents
Cxcl13 antibody (PB9999) performs well in ELISA, IHC, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of BCA1/CXCL13 using anti-BCA1/CXCL13 antibody (PB9999).
BCA1/CXCL13 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCA1/CXCL13 Antibody (PB9999) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
BCA1/CXCL13 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCA1/CXCL13 Antibody (PB9999) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-BCA1/CXCL13 Antibody Picoband®
Cxcl13 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | ELISA, IHC, WB |
Tested Applications
Cxcl13 antibody was positively detected by WB in:
recombinant mouse BCA1/CXCL13 protein
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Cxcl13 antibody was positively detected by IHC in:
mouse spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-BCA1/CXCL13 Antibody Picoband®
View all CXCL13 Antibodies
View all CXCL13 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109). Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated Molecular Weight
11.9 kDa
Background of Cxcl13
C-X-C motif chemokine 13 (CXCL13), also known as B cell-attracting chemokine 1 (BCA-1), is a protein that in humans is encoded by the CXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family. Its gene is mapped to human chromosome 4 in a cluster of other CXC chemokines. This protein has contained 109 amino acids. It is expressed highly in the liver, spleen, lymph nodes and gut of humans. The protein induces chemotaxis of BLR1-expressing B cells.
Cite This Product
Anti-BCA1/CXCL13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9999)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
67%
Low homology
Dog
49%
Low homology
Monkey
49%
Low homology
Rabbit
48%
Low homology
Cow
47%
Low homology
Sheep
47%
Low homology
Human
44%
Low homology
Zebrafish
23%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Cxcl13 antibody
Immunohistochemistry (IHC) protocol for Cxcl13 antibody
Publications
Application:
Species:
Multi-marker algorithms based on CXCL13, IL-10, sIL-2 receptor, and β2-microglobulin in cerebrospinal fluid to diagnose CNS lymphoma
Published: 2020-04-20
Journal: Cancer Medicine
Application: IHC
Impact Factor: 3.49
Species: Human
PMID: 32314548
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Product Promise
We promise Anti-BCA1/CXCL13 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9999
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026