Anti-Bcl-2-like protein 2 Bcl2L2 Antibody

BCL-W/BCL2L2 antibody

Boster Bio Anti-Bcl-2-like protein 2 Bcl2L2 Antibody catalog # PA1426-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA1426-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, ICC, WB

Product Name

Anti-Bcl-2-like protein 2 Bcl2L2 Antibody

View all BCL-W/BCL2L2 Antibodies

SKU/Catalog Number

PA1426-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Bcl-2-like protein 2 Bcl2L2 Antibody catalog # PA1426-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bcl-2-like protein 2 Bcl2L2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1426-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human BCL2L2, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1426-1 is reactive to BCL2L2 in Human, Mouse, Rat

Applications

PA1426-1 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

21 kDa

Calculated molecular weight

20.746kDa

Background of BCL-W/BCL2L2

Bcl-2-like protein 2 is a protein that in humans is encoded by the BCL2L2 gene. This gene encodes a pro-survival (anti-apoptotic) member of the bcl-2 protein family. Its gene is mapped to human chromosome 14q11.2-q12. In all, it has got 193 amino acids. Generally speaking, the BCL2 can be found in brain, colon and salivary gland. Acting as the anti- and pro-apoptotic regulators, it has played a vital role in reducing the cell apoptosis under cytotoxic conditions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For BCL2L2 (Source: Uniprot.org, NCBI)

Gene Name

BCL2L2

Full Name

Bcl-2-like protein 2

Weight

20.746kDa

Superfamily

Bcl-2 family

Alternative Names

Apoptosis regulator Bcl-W; BCL2L2; Bcl2-L-2; BCL2-like 2; Bclw; Bcl-w; BCLWbcl-2-like protein 2; KIAA0271BCL2-L-2 BCL2L2 BCL-W, BCL2-L-2, BCLW, PPP1R51 BCL2 like 2 bcl-2-like protein 2|apoptosis regulator BCL-W|protein phosphatase 1, regulatory subunit 51

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BCL2L2, check out the BCL2L2 Infographic

BCL2L2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BCL2L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1426-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse
PA1426-1 usage in article: APP:WB, SAMPLE:SNU-16 CELL, DILUTION:NA

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4 Customer Q&As for Anti-Bcl-2-like protein 2 Bcl2L2 Antibody

Question

I see that the anti-Bcl2L2 antibody PA1426-1 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-26

Question

We are currently using anti-Bcl2L2 antibody PA1426-1 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

R. Miller

Verified customer

Asked: 2019-11-07

Answer

The anti-Bcl2L2 antibody (PA1426-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-07

Question

I was wanting to use your anti-Bcl2L2 antibody for Flow Cytometry for rat leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

As indicated on the product datasheet, PA1426-1 anti-Bcl2L2 antibody has been validated for Flow Cytometry, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-20

Question

Will anti-Bcl2L2 antibody PA1426-1 work for Flow Cytometry with leukemic t-cell?

C. Moore

Verified customer

Asked: 2015-10-16

Answer

According to the expression profile of leukemic t-cell, BCL2L2 is highly expressed in leukemic t-cell. So, it is likely that anti-Bcl2L2 antibody PA1426-1 will work for Flow Cytometry with leukemic t-cell.

Boster Scientific Support

Answered: 2015-10-16

Order DetailsPrice
PA1426-1

100μg

$370
PA1426-1-10ug

10μg sample (liquid)

$99
PA1426-1-Biotin

100 μg Biotin conjugated

$570
PA1426-1-Cy3

100 μg Cy3 conjugated

$570
PA1426-1-Dylight488

100 μg Dylight488 conjugated

$570
PA1426-1-Dylight550

100 μg Dylight550 conjugated

$570
PA1426-1-Dylight594

100 μg Dylight594 conjugated

$570
PA1426-1-FITC

100 μg FITC conjugated

$570
PA1426-1-HRP

100 μg HRP conjugated

$570
PA1426-1-APC

100 μg APC conjugated

$670
PA1426-1-PE

100 μg PE conjugated

$670
PA1426-1-carrier-free

Carrier Free

$370

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PA1426-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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