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Product Info Summary
| SKU: | A02161 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Brachyury Antibody
SKU/Catalog Number
A02161
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-Brachyury Antibody (Catalog# A02161). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-Brachyury Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02161)
Host
Rabbit
Contents
100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A partial length recombinant Brachyury protein (amino acids 200-410)
Cross-reactivity
Predicted species reactivity based on epitope sequence homology: human, mouse, bovine, horse, rhesus macaque, dog, chimpanzee.
Reactive Species
A02161 is reactive to TBXT in Human
Calculated molecular weight
47.4 kDa
Background of TBXT
NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02161 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
Validation Images & Assay Conditions
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Western blot analysis of Brachyury. Anti-Brachyury antibody was used at 4 &mu
Specific Publications For Anti-Brachyury Antibody (A02161)
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Customer Q&As
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6 Customer Q&As for Anti-Brachyury Antibody
Question
Do you have a BSA free version of anti-Brachyury antibody A02161 available?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Brachyury antibody A02161 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-20
Question
My lab would like to test anti-Brachyury antibody A02161 on human brain {eco:0000313 for research purposes, then I may be interested in using anti-Brachyury antibody A02161 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
The products we sell, including anti-Brachyury antibody A02161, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-23
Question
Would A02161 anti-Brachyury antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
You can see on the product datasheet, A02161 anti-Brachyury antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-28
Question
I have attached the WB image, lot number and protocol we used for brain {eco:0000313 using anti-Brachyury antibody A02161. Please let me know if you require anything else.
T. Yang
Verified customer
Asked: 2017-06-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-07
Question
I was wanting to use your anti-Brachyury antibody for WB for human brain eco:0000313 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been validated and is this antibody a good choice for human brain {eco:0000313 identification?
P. Huang
Verified customer
Asked: 2016-10-26
Answer
As indicated on the product datasheet, A02161 anti-Brachyury antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain {eco:0000313 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-10-26
Question
We are currently using anti-Brachyury antibody A02161 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
J. Krishna
Verified customer
Asked: 2015-05-07
Answer
The anti-Brachyury antibody (A02161) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-07
