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Product Info Summary
| SKU: | A01564 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-BubR1 BUB1B Antibody
SKU/Catalog Number
A01564
Size
100μl
Form
Liquid
Description
Boster Bio Anti-BubR1 BUB1B Antibody catalog # A01564. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BubR1 BUB1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01564)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human BUB1B. AA range:341-390
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01564 is reactive to BUB1B in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
119.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01564 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using BubR1 Polyclonal Antibody
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Western blot analysis of lysates from HeLa and HepG2 cells, treated with H2O2 100uM 30', using BUB1B Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-BubR1 BUB1B Antibody (A01564)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-BubR1 BUB1B Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-BubR1 antibody A01564. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-04
Question
Is this A01564 anti-BubR1 antibody reactive to the isotypes of BUB1B?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The immunogen of A01564 anti-BubR1 antibody is Synthesized peptide derived from the Internal region of human BubR1. at AA rangle: 310-390. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-01
Question
Can you help my question with product A01564, anti-BubR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01564 anti-BubR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-10
Question
Does anti-BubR1 antibody A01564 work for WB with cervix carcinoma?
C. Johnson
Verified customer
Asked: 2016-05-05
Answer
According to the expression profile of cervix carcinoma, BUB1B is highly expressed in cervix carcinoma. So, it is likely that anti-BubR1 antibody A01564 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2016-05-05
Question
I was wanting to use your anti-BubR1 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?
G. Banerjee
Verified customer
Asked: 2016-03-02
Answer
You can see on the product datasheet, A01564 anti-BubR1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-02
