Anti-BubR1/BUB1B Antibody Picoband™ (monoclonal, 8B3)

BubR1 antibody

Boster Bio Anti-BubR1/BUB1B Antibody Picoband™ (monoclonal, 8B3) catalog # M01564-3. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: M01564-3
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Mouse
Application: Flow Cytometry, IHC, WB

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Product Name

Anti-BubR1/BUB1B Antibody Picoband™ (monoclonal, 8B3)

View all BubR1 Antibodies

SKU/Catalog Number

M01564-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BubR1/BUB1B Antibody Picoband™ (monoclonal, 8B3) catalog # M01564-3. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BubR1/BUB1B Antibody Picoband™ (monoclonal, 8B3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01564-3)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

8B3

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human BubR1/BUB1B recombinant protein (Position: K26-E448).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M01564-3 is reactive to BUB1B in Human, Rat

Applications

M01564-3 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Background of BubR1

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta?is an?enzyme?that in humans is encoded by the?BUB1B?gene. This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For BUB1B (Source: Uniprot.org, NCBI)

Uniprot ID

O60566

Gene ID

701

Gene Name

BUB1B

Full Name

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Weight

119.545kDa

Superfamily

protein kinase superfamily

Alternative Names

Bub1A; BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta; budding uninhibited by benzimidazoles 1 homolog beta (yeast); EC 2.7.11.1; hBUBR1; MAD3/BUB1-related protein kinase; MAD3LBUB1beta; Mitotic checkpoint kinase MAD3L; Protein SSK1; SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta BUB1B BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1 BUB1 mitotic checkpoint serine/threonine kinase B mitotic checkpoint serine/threonine-protein kinase BUB1 beta|BUB1B, mitotic checkpoint serine/threonine kinase|MAD3/BUB1-related protein kinase|budding uninhibited by benzimidazoles 1 homolog beta|mitotic checkpoint kinase MAD3L

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BUB1B, check out the BUB1B Infographic

BUB1B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BUB1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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Total: $315

SKU:M01564-3

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M01564-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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