Anti-BubR1/BUB1B Antibody Picoband™ (monoclonal, 8B3)

BubR1 antibody

Boster Bio Anti-BubR1/BUB1B Antibody Picoband™ (monoclonal, 8B3) catalog # M01564-3. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: M01564-3
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Mouse
Application: Flow Cytometry, IHC, WB

Product Name

Anti-BubR1/BUB1B Antibody Picoband™ (monoclonal, 8B3)

View all BubR1 Antibodies

SKU/Catalog Number

M01564-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BubR1/BUB1B Antibody Picoband™ (monoclonal, 8B3) catalog # M01564-3. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BubR1/BUB1B Antibody Picoband™ (monoclonal, 8B3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01564-3)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

8B3

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human BubR1/BUB1B recombinant protein (Position: K26-E448).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M01564-3 is reactive to BUB1B in Human, Rat

Applications

M01564-3 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

130 kDa

Calculated molecular weight

119.545kDa

Background of BubR1

Mitotic checkpoint serine/threonine-protein kinase BUB1 betais anenzymethat in humans is encoded by theBUB1Bgene. This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For BUB1B (Source: Uniprot.org, NCBI)

Gene Name

BUB1B

Full Name

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Weight

119.545kDa

Superfamily

protein kinase superfamily

Alternative Names

Bub1A; BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta; budding uninhibited by benzimidazoles 1 homolog beta (yeast); EC 2.7.11.1; hBUBR1; MAD3/BUB1-related protein kinase; MAD3LBUB1beta; Mitotic checkpoint kinase MAD3L; Protein SSK1; SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta BUB1B BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1 BUB1 mitotic checkpoint serine/threonine kinase B mitotic checkpoint serine/threonine-protein kinase BUB1 beta|BUB1B, mitotic checkpoint serine/threonine kinase|MAD3/BUB1-related protein kinase|budding uninhibited by benzimidazoles 1 homolog beta|mitotic checkpoint kinase MAD3L

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BUB1B, check out the BUB1B Infographic

BUB1B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BUB1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M01564-3

100μg

$370
M01564-3-10ug

10μg sample (liquid)

$99
M01564-3-Biotin

100 μg Biotin conjugated

$570
M01564-3-Cy3

100 μg Cy3 conjugated

$570
M01564-3-Dylight488

100 μg Dylight488 conjugated

$570
M01564-3-Dylight550

100 μg Dylight550 conjugated

$570
M01564-3-Dylight594

100 μg Dylight594 conjugated

$570
M01564-3-FITC

100 μg FITC conjugated

$570
M01564-3-HRP

100 μg HRP conjugated

$570
M01564-3-APC

100 μg APC conjugated

$670
M01564-3-PE

100 μg PE conjugated

$670
M01564-3-carrier-free

Carrier Free

$370

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M01564-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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