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- Table of Contents
FOS antibody (M00297) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IF analysis of c-Fos using anti-c-Fos antibody (M00297).
c-Fos was detected in an immunocytochemical section of mouse brain tissue. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1:200 rabbit anti-c-Fos Antibody (M00297) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
c-Fos was detected in an immunocytochemical section of mouse brain tissue. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1:200 rabbit anti-c-Fos Antibody (M00297) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-c-Fos Rabbit Monoclonal Antibody
FOS antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, WB |
Tested Applications
FOS antibody was positively detected by WB in:
human Hela whole cell, human RT4 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Recommend
Recommend
Mouse
Recommend
Recommend
Rat
Recommend
Recommend
Product Information
Product Name
Anti-c-Fos Rabbit Monoclonal Antibody
View all FOS Antibodies
View all FOS Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IIO-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human c-Fos
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
41 kDa
Calculated Molecular Weight
40.7 kDa
Cite This Product
Anti-c-Fos Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00297)
Protocols
Western Blot protocol for FOS antibody
Publications
Application:
Species:
Injectable hydrogel bioelectrostimulator for wireless deep brain neuromodulation
Published: 2026-02-04
Journal: Nature Communications
Application: IHC
Impact Factor: 15.70
Species: Rat
PMID: 41639090
Molecular characterization of PANoptosis-related genes in chronic kidney disease
Published: 2024-10-28
Journal: PLoS One
Application: WB
Impact Factor: 2.90
Species: Rat
PMID: 39466748
Tumor suppressive role of miR-569 in lung cancer
Published: 2018-01-26
Journal: Oncology Letters
Application: WB
Impact Factor: 1.66
Species: Human
PMID: 29541173
Review
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2 Reviews For Anti-c-Fos Rabbit Monoclonal Antibody
The Anti-c-FOS antibody (M00297) showed clear and specific immunofluorescence staining in mouse brain, with markedly increased c-Fos expression in neurons of the acute ischemic region compared to normal brain.
| SKU | M00297 |
|---|---|
| Application | Immunocytochemistry |
| Sample | mouse brain |
| Sample Processing Description | Normal brain tissue and brain tissue from a cerebral infarction model |
| Other Reagents | Goat serum, DAB Chromogenic Solution, Anti-fade mounting medium |
| Primary Antibody | c-Fos Rabbit Monoclonal Antibody |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Secondary Antibody | DyLight 594 Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 45 min at 37℃ |
| Detection | Imaging system:Leica DM2500 |
| Results Summary | c-Fos is a marker of activated neural cells, especially neurons. During the acute phase of cerebral infarction, as a strong stress and injury stimulus, it drives c-Fos expression in the brain—particularly in the peri-infarct region—far higher than in normal resting brain. The experimental results show clear staining of positive cells, and the expression levels are consistent with expectations. |
Qihang Yang, Huazhong University of Science and Technology
Verified customer
Submitted 2026-02-12
This antibody demonstrates good specificity, with minimal nonspecific bands. The target band is clear and at the correct position, and expression differences between different groups are observable.
| SKU | M00297 |
|---|---|
| Application | Western Blot |
| Sample | Mouse hippocampal tissue |
| Sample Processing Description | An Alzheimer’s disease (AD) model was established by injecting streptozotocin (STZ) into the lateral ventricles of mice. Hippocampal tissues were collected after treatment with four different doses of QianCengTa compound, and total protein was extracted. |
| Other Reagents | RIPA lysis buffer,Protease inhibitor,Electrophoresis buffer,Transfer buffer,Blocking buffer |
| Primary Antibody | c-Fos Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Goat Anti-Rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | c-FOS is a marker of neuronal activation under pathological conditions. Its expression is low in normal brain tissue but elevated in the AD model, and decreases following drug treatment. Lane 1 represents normal hippocampus, lane 2 the AD model hippocampus, lanes 3, 4, and 5 correspond to increasing doses of QianCengTa compound, and lane 6 is the positive control drug. The Western blot results indicate that QianCengTa compound exhibits a therapeutic effect on AD. |
Xiangnan Qin, SHUTCM
Verified customer
Submitted 2025-12-25
Product Promise
We promise Anti-c-Fos Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00297
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026