Anti-NGF/Beta Ngf Rabbit Monoclonal Antibody

beta-NGF antibody

Boster Bio Anti-NGF/Beta Ngf Rabbit Monoclonal Antibody catalog # M00341. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat. Cited in 5 publication(s).

Product Info Summary

SKU: M00341
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

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Product Name

Anti-NGF/Beta Ngf Rabbit Monoclonal Antibody

View all beta-NGF Antibodies

SKU/Catalog Number

M00341

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-NGF/Beta Ngf Rabbit Monoclonal Antibody catalog # M00341. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NGF/Beta Ngf Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00341)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BDE-14

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human NGF

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00341 is reactive to NGF in Human, Mouse, Rat

Applications

M00341 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

26.959kDa

Background of beta-NGF

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For NGF (Source: Uniprot.org, NCBI)

Gene Name

NGF

Full Name

Beta-nerve growth factor

Weight

26.959kDa

Superfamily

NGF-beta family

Alternative Names

Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428 NGF Beta-NGF, HSAN5B, NGF nerve growth factor beta-nerve growth factor|nerve growth factor (beta polypeptide)|nerve growth factor, beta subunit|pro-nerve growth factor long

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NGF, check out the NGF Infographic

NGF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00341 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury

Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats

Associations between proinflammatory cytokines in the synovial fluid and radiographic grading and pain-related scores in 47 consecutive patients with osteoarthritis of the knee

Huang S, Zhang X, Xu L, Li Q, Zhao Q. Exp Ther Med. 2014 Oct;8(4):1237-1240. Epub 2014 Jul 21. Expression Of Nerve Growth Factor In The Prostate Of Male Rats In Response To Chronic Stress And Sympathetic Denervation.

Luo Q, Liu W, Chen J, Wang M, Zeng W, Chen Z, Cheng A. Neural Regen Res. 2012 Feb 5;7(4):302-7. Doi: 10.3969/J.Issn.1673-5374.2012.04.011. Nerve Growth Factor And Inducible Nitric Oxide Synthase Expression In The Mesencephalon And Diencephalon, As...

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5 Customer Q&As for Anti-NGF/Beta Ngf Rabbit Monoclonal Antibody

Question

We purchased anti-NGF/Beta Ngf Rabbit Monoclonal antibody for IHC on cartilage tissue a few months ago. I am using mouse, and We are going to use the antibody for IF next. I am interested in examining cartilage tissue as well as leukocyte in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

I have checked the website and datasheets of our anti-NGF/Beta Ngf Rabbit Monoclonal antibody and I see that M00341 has been validated on mouse in both IHC and IF. Thus M00341 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-12-09

Question

We are currently using anti-NGF/Beta Ngf Rabbit Monoclonal antibody M00341 for mouse tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

The anti-NGF/Beta Ngf Rabbit Monoclonal antibody (M00341) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-27

Question

Our team were well pleased with the WB result of your anti-NGF/Beta Ngf Rabbit Monoclonal antibody. However we have seen positive staining in eye secreted. using this antibody. Is that expected? Could you tell me where is NGF supposed to be expressed?

S. Mangal

Verified customer

Asked: 2019-09-09

Answer

According to literature, eye does express NGF. Generally NGF expresses in secreted. Regarding which tissues have NGF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2374737
Eye, Pubmed ID: 15489334
Leukocyte, Pubmed ID: 2025430

Boster Scientific Support

Answered: 2019-09-09

Question

We need using your anti-NGF/Beta Ngf Rabbit Monoclonal antibody for positive regulation of neuron differentiation studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

E. Taylor

Verified customer

Asked: 2018-11-19

Answer

We appreciate your inquiry. This M00341 anti-NGF/Beta Ngf Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-11-19

Question

We have seen staining in rat cartilage tissue. Do you have any suggestions? Is anti-NGF/Beta Ngf Rabbit Monoclonal antibody supposed to stain cartilage tissue positively?

Verified Customer

Verified customer

Asked: 2018-08-01

Answer

According to literature cartilage tissue does express NGF. According to Uniprot.org, NGF is expressed in cartilage tissue, brain, eye, leukocyte, among other tissues. Regarding which tissues have NGF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2374737
Eye, Pubmed ID: 15489334
Leukocyte, Pubmed ID: 2025430

Boster Scientific Support

Answered: 2018-08-01

Order DetailsPrice
M00341

100μL

$370
M00341-30ul

30μL sample

$99
M00341-carrier-free

Carrier Free

$370

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