Anti-c-Myc Antibody (Monoclonal, 9E10)

Boster Bio Anti-c-Myc Antibody (Monoclonal, 9E10) catalog # MA1028. Tested in IHC, ICC, WB applications. This antibody reacts with Human. Cited in 16 publication(s).

Product Info Summary

SKU: MA1028
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC, ICC, WB

Product Name

Anti-c-Myc Antibody (Monoclonal, 9E10)

See all c-Myc products

SKU/Catalog Number

MA1028

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-c-Myc Antibody (Monoclonal, 9E10) catalog # MA1028. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-c-Myc Antibody (Monoclonal, 9E10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1028)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

9.00E+10

Isotype

Mouse IgG1

Immunogen

Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1028 is reactive to Myc in Human

Applications

MA1028 is guaranteed for IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 8μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Western blot, 4μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For Myc (Source: Uniprot.Org, NCBI)

Uniprot ID

P09416

Gene ID

24577

Gene Name

Myc

Full Name

Myc proto-oncogene protein

Weight

48.898kDa

Alternative Names

9E10 Myc; 9E10 Myc; avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian) Myc|RNCMYC, c-myc, mMyc|MYC proto-oncogene, bHLH transcription factor|myc proto-oncogene protein|Avian myelocytomatosis viral (v-myc) oncogene homolog|myelocytomatosis oncogene|myelocytomatosis viral oncogene homolog|proto-oncogene c-Myc|transcription factor p64|v-myc avian myelocytomatosis viral oncogene homolog

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Myc, check out the Myc Infographic

Myc infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Myc: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1028 has been cited in 16 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Long non-coding RNA LINC01503 predicts worse prognosis in glioma and promotes tumorigenesis and progression through activation of Wnt/β-catenin signaling H Wang 1, Z-G Sheng, L-Z Dai Eur Rev Med Pharmacol Sci. 2019 Feb;23(4):1600-1609. doi: 10.26355/e

Inhibition of N-myc downstream-regulated gene 2 in prostatic carcinoma

Arginine deiminase expressed in vivo, driven by human telomerase reverse transcriptase promoter, displays high hepatoma targeting and oncolytic efficiency

Tumor suppressor NDRG2 inhibits glycolysis and glutaminolysis in colorectal cancer cells by repressing c-Myc expression

Blockage of Glyoxalase I Inhibits Colorectal Tumorigenesis and Tumor Growth via Upregulation of STAT1, p53, and Bax and Downregulation of c-Myc and Bcl-2

Wang Ph, Gu Zh, Wan Dh, Zhu Wb, Qiu W, Weng Sp, Yu Xq, He Jg. Plos One. 2013;8(2):E52088. Doi: 10.1371/Journal.Pone.0052088. Epub 2013 Feb 6. Litopenaeus Vannamei Sterile-Alpha And Armadillo Motif Containing Protein (Lvsarm) Is Involved In Regulat...

Long Xb, Sun Gb, Hu S, Liang Gt, Wang N, Zhang Xh, Cao Pp, Zhen Ht, Cui Yh, Liu Z. Oncol Rep. 2009 Nov;22(5):1189-95. Let-7A Microrna Functions As A Potential Tumor Suppressor In Human Laryngeal Cancer.

Liu B, Liu F, Bai L, Li Y, Yang G. Mol Biol Rep. 2010 Mar;37(3):1671-6. Doi: 10.1007/S11033-009-9858-Y. Epub 2009 Oct 13. Molecular Cloning, Expression And Subcellular Distribution Of An Alternative Splice Variant Of The Porcine Sirt2 Gene.

Zhang K, Lu Y, Yang P, Li C, Sun H, Tao D, Liu Y, Zhang S, Ma Y. Plos One. 2012;7(7):E41973. Doi: 10.1371/Journal.Pone.0041973. Epub 2012 Jul 27. Hili Inhibits Tgf-?? Signaling By Interacting With Hsp90 And Promoting T??r Degradation.

Hao Hp, Wen Lb, Li Jr, Wang Y, Ni B, Wang R, Wang X, Sun Mx, Fan Hj, Mao X. Antiviral Res. 2015 May;117:99-109. Doi: 10.1016/J.Antiviral.2015.02.010. Epub 2015 Mar 5. Licl Inhibits Prrsv Infection By Enhancing Wnt/??-Catenin Pathway And Suppressin...

Wang H, Huo N, Li F, Fu S, Xue Y, Yang T, Wen X, Ding Y, Duan X. Mol Cell Biochem. 2010 Sep;342(1-2):191-9. Doi: 10.1007/S11010-010-0483-9. Epub 2010 May 16. Osteogenic Role Of Endosomal Chloride Channels In Mc3T3-E1 Cells.

Zhao P, Li Y, Gao G, Wang S, Yan Y, Zhan X, Liu Z, Mao Z, Chen S, Wang L. Eur J Med Chem. 2014 Oct 30;86:165-74. Doi: 10.1016/J.Ejmech.2014.08.049. Epub 2014 Aug 15. Design, Synthesis And Biological Evaluation Of N-Alkyl Or Aryl Substituted Isoind...

Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...

Yuan Y, Zhang J, Cai L, Ding C, Wang X, Chen H, Wang X, Yan J, Lu J. Oncol Rep. 2013 Jun;29(6):2291-6. Doi: 10.3892/Or.2013.2390. Epub 2013 Apr 8. Leptin Induces Cell Proliferation And Reduces Cell Apoptosis By Activating C-Myc In Cervical Cancer.

Qin Xg, Hua Z, Shuang W, Wang Yh, Cui Yd. Pharm Biol. 2010 Mar;48(3):275-81. Doi: 10.3109/13880200903104101. Effects Of Matrine On Hepg2 Cell Proliferation And Expression Of Tumor Relevant Proteins In Vitro.

Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.

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17 Customer Q&As for Anti-c-Myc Antibody (Monoclonal, 9E10)

Question

I have attached the WB image, lot number and protocol we used for promyelocytic leukemia using anti-c-Myc antibody (Monoclonal, 9E10) MA1028. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-23

Question

I see that the anti-c-Myc antibody (Monoclonal, 9E10) MA1028 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-07

Question

Will MA1028 anti-c-Myc antibody (Monoclonal, 9E10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

As indicated on the product datasheet, MA1028 anti-c-Myc antibody (Monoclonal, 9E10) as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-11-19

Question

you antibody to test anti-c-Myc antibody (Monoclonal, 9E10) MA1028 on human promyelocytic leukemia for research purposes, then I may be interested in using anti-c-Myc antibody (Monoclonal, 9E10) MA1028 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

R. Li

Verified customer

Asked: 2019-09-27

Answer

The products we sell, including anti-c-Myc antibody (Monoclonal, 9E10) MA1028, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-27

Question

We bought anti-c-Myc antibody (Monoclonal, 9E10) for WB on cervix a few years ago. I am using human, and We intend to use the antibody for IHC next. I am interested in examining cervix as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

I have checked the website and datasheets of our anti-c-Myc antibody (Monoclonal, 9E10) and it seems that MA1028 has been tested on human in both WB and IHC. Thus MA1028 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-24

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for promyelocytic leukemia using anti-c-Myc antibody (Monoclonal, 9E10) MA1028. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-26

Question

Will anti-c-Myc antibody (Monoclonal, 9E10) MA1028 work for IHC with promyelocytic leukemia?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

According to the expression profile of promyelocytic leukemia, MYC is highly expressed in promyelocytic leukemia. So, it is likely that anti-c-Myc antibody (Monoclonal, 9E10) MA1028 will work for IHC with promyelocytic leukemia.

Boster Scientific Support

Answered: 2019-05-24

Question

Is a blocking peptide available for product anti-c-Myc antibody (Monoclonal, 9E10) (MA1028)?

Verified Customer

Verified customer

Asked: 2018-04-09

Answer

We do provide the blocking peptide for product anti-c-Myc antibody (Monoclonal, 9E10) (MA1028). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-04-09

Question

Is this MA1028 anti-c-Myc antibody (Monoclonal, 9E10) reactive to the isotypes of MYC?

Verified Customer

Verified customer

Asked: 2018-02-09

Answer

The immunogen of MA1028 anti-c-Myc antibody (Monoclonal, 9E10) is Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-02-09

Question

Is there a BSA free version of anti-c-Myc antibody (Monoclonal, 9E10) MA1028 available?

A. Zhao

Verified customer

Asked: 2017-12-21

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-c-Myc antibody (Monoclonal, 9E10) MA1028 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-12-21

Question

My question regarding product MA1028, anti-c-Myc antibody (Monoclonal, 9E10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-12-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1028 anti-c-Myc antibody (Monoclonal, 9E10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-12-15

Question

Will anti-c-Myc antibody (Monoclonal, 9E10) MA1028 work on zebrafish IHC with cervix?

C. Wu

Verified customer

Asked: 2017-03-29

Answer

Our lab technicians have not validated anti-c-Myc antibody (Monoclonal, 9E10) MA1028 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-c-Myc antibody (Monoclonal, 9E10) MA1028 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-03-29

Question

We need using your anti-c-Myc antibody (Monoclonal, 9E10) for response to gamma radiation studies. Has this antibody been tested with western blotting on cardiac muscle tissue? We would like to see some validation images before ordering.

N. Dhar

Verified customer

Asked: 2016-12-30

Answer

Thank you for your inquiry. This MA1028 anti-c-Myc antibody (Monoclonal, 9E10) is validated on cardiac muscle tissue. It is guaranteed to work for IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-12-30

Question

I was wanting to use your anti-c-Myc antibody (Monoclonal, 9E10) for IHC for human promyelocytic leukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human promyelocytic leukemia identification?

J. Roberts

Verified customer

Asked: 2015-05-14

Answer

As indicated on the product datasheet, MA1028 anti-c-Myc antibody (Monoclonal, 9E10) has been tested for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human promyelocytic leukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-05-14

Question

We are currently using anti-c-Myc antibody (Monoclonal, 9E10) MA1028 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?

K. Banerjee

Verified customer

Asked: 2014-11-24

Answer

The anti-c-Myc antibody (Monoclonal, 9E10) (MA1028) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-11-24

Question

We have observed staining in human cervix. What should we do? Is anti-c-Myc antibody (Monoclonal, 9E10) supposed to stain cervix positively?

G. Yang

Verified customer

Asked: 2014-09-05

Answer

From what I have seen in literature cervix does express MYC. From what I have seen in Uniprot.org, MYC is expressed in thoracic mammary gland, uterus, cervix, placenta testis, promyelocytic leukemia, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2014-09-05

Question

We were satisfied with the WB result of your anti-c-Myc antibody (Monoclonal, 9E10). However we have been able to see positive staining in cervix carcinoma erythroleukemia nucleoplasm using this antibody. Is that expected? Could you tell me where is MYC supposed to be expressed?

C. Thomas

Verified customer

Asked: 2013-01-22

Answer

According to literature, cervix carcinoma erythroleukemia does express MYC. Generally MYC expresses in nucleus, nucleoplasm. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2013-01-22

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