|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-c-Myc Antibody (Monoclonal, 9E10)|
|Description||Mouse IgG monoclonal antibody for c-Myc, v-myc myelocytomatosis viral oncogene homolog (avian) (MYC) detection. Tested with WB, IHC-P, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-c-Myc Antibody (Monoclonal, 9E10) 9E10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1028)|
|Replacement Item||This antibody may replace the following items: sc-28207 from Santa Cruz Biotechnology.|
|Predicted Species||Monkey, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Myc proto-oncogene protein|
|Molecular Weight||48898 MW|
|Protein Function||Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. .|
|Sequence Similarities||Contains 1 bHLH (basic helix-loop-helix) domain.|
|Subcellular Localization||Nucleus, nucleoplasm . Nucleus, nucleolus .|
|Alternative Names||Myc proto-oncogene protein;Proto-oncogene c-Myc;Transcription factor p64;Myc;|
|Research Areas|||epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper| stem cells|signaling pathways|tgf beta|nuclear| epigenetics and nuclear signaling|transcription factors| cancer|cell cycle|cell differentiation|oncoproteins/suppressors|oncoproteins|tumor biomarkers||
Background for Myc proto-oncogene protein
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-c-Myc Antibody (Monoclonal, 9E10) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 8μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Western blot, 4μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-c-Myc Antibody (Monoclonal, 9E10) Images
Click the images to enlarge.
IHC(P): Rat Cardiac Muscle Tissue