|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-c-Myc Antibody (Monoclonal, 9E10)|
|Description||Mouse IgG monoclonal antibody for c-Myc, v-myc myelocytomatosis viral oncogene homolog (avian) (MYC) detection. Tested with WB, IHC-P, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-c-Myc Antibody (Monoclonal, 9E10) 9E10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1028)|
|Replacement Item||This antibody may replace the following items: sc-28207 from Santa Cruz Biotechnology.|
|Predicted Species||Monkey, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Myc proto-oncogene protein|
|Molecular Weight||48898 MW|
|Protein Function||Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. .|
|Sequence Similarities||Contains 1 bHLH (basic helix-loop-helix) domain.|
|Subcellular Localization||Nucleus, nucleoplasm . Nucleus, nucleolus .|
|Alternative Names||Myc proto-oncogene protein;Proto-oncogene c-Myc;Transcription factor p64;Myc;|
|Research Areas|||epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper| stem cells|signaling pathways|tgf beta|nuclear| epigenetics and nuclear signaling|transcription factors| cancer|cell cycle|cell differentiation|oncoproteins/suppressors|oncoproteins|tumor biomarkers||
Background for Myc proto-oncogene protein
Anti-c-Myc Antibody (Monoclonal, 9E10) Images
Click the images to enlarge.
IHC(P): Rat Cardiac Muscle Tissue