|Ig Isotype||Mouse IgG1|
|Applications||IHC, ICC, WB|
|Product Name||Anti-c-Myc Antibody (Monoclonal, 9E10)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for c-Myc, v-myc myelocytomatosis viral oncogene homolog (avian) (MYC) detection. Tested with WB, IHC-P, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-c-Myc Antibody (Monoclonal, 9E10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1028)|
|Immunogen||Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 8μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Western blot, 4μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
Images And Assay Conditions
Anti-c-Myc antibody (monoclonal), MA1028, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Myc proto-oncogene protein|
|Alternative Names||Myc proto-oncogene protein;Proto-oncogene c-Myc;Transcription factor p64;Myc;|
|Subcellular Localization||Nucleus, nucleoplasm . Nucleus, nucleolus .|
|Molecular Weight||48898 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. .|
|Research Areas||Cancer, Cell Cycle, Cell Differentiation, Domain Families, Epigenetics And Nuclear Signaling, Hlh / Leucine Zipper, Nuclear, Oncoproteins, Oncoproteins/Suppressors, Signaling Pathways, Stem Cells, Tgf Beta, Transcription, Transcription Factors, Tumor Biomarkers
*You can search these to find other products in these research areas.
|Background||C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol(2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.|
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