SKU PA1555
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-c-Myc Antibody
SKU/Catalog Number PA1555
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Myc proto-oncogene protein(MYC) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-c-Myc Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1555)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human c-Myc(421-439aa RKRREQLKHKLEQLRNSCA), different from the related mouse and rat sequences by one amino acid.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

Anti-c-Myc antibody, PA1555, Western blotting
All lanes: Anti c-Myc(PA1555) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P01106
Gene Name MYC
Protein Name Myc proto-oncogene protein
Alternative Names Myc proto-oncogene protein;Class E basic helix-loop-helix protein 39;bHLHe39;Proto-oncogene c-Myc;Transcription factor p64;MYC;BHLHE39;
Subcellular Localization Nucleus, nucleoplasm . Nucleus, nucleolus .
Molecular Weight 48804 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes.
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol(2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Polyclonal antibody for C MYC/MYC detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. C MYC/MYC information: Molecular Weight: 48804 MW; Subcellular Localization: Nucleus, nucleoplasm . Nucleus, nucleolus .
Write Your Own Review
Only registered users can write reviews. Please Sign in or create an account
In stock
Order Product
PA1555
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

troubleshooting_box_image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Publications

Constitutive %u03B2-catenin activation in osteoblasts impairs terminal osteoblast differentiation and bone quality
Inhibition of N-myc downstream-regulated gene 2 in prostatic carcinoma
Arginine deiminase expressed in vivo, driven by human telomerase reverse transcriptase promoter, displays high hepatoma targeting and oncolytic efficiency
Tumor suppressor NDRG2 inhibits glycolysis and glutaminolysis in colorectal cancer cells by repressing c-Myc expression
Metformin Inhibits Tumorigenesis and Tumor Growth of Breast Cancer Cells by Upregulating miR-200c but Downregulating AKT2 Expression
Blockage of Glyoxalase I Inhibits Colorectal Tumorigenesis and Tumor Growth via Upregulation of STAT1, p53, and Bax and Downregulation of c-Myc and Bcl-2
Zhang D, Fei F, Li S, Zhao Y, Yang Z, Qu J, Zhang X, Yin Y, Zhang S. J Cancer. 2017 Jul 5;8(11):2114-2123. doi: 10.7150/jca.19723. eCollection 2017. The role of β-catenin in the initiation and metastasis of TA2 mice spontaneous breast cancer
Wang Ph, Gu Zh, Wan Dh, Zhu Wb, Qiu W, Weng Sp, Yu Xq, He Jg. Plos One. 2013;8(2):E52088. Doi: 10.1371/Journal.Pone.0052088. Epub 2013 Feb 6. Litopenaeus Vannamei Sterile-Alpha And Armadillo Motif Containing Protein (Lvsarm) Is Involved In Regulat...
Long Xb, Sun Gb, Hu S, Liang Gt, Wang N, Zhang Xh, Cao Pp, Zhen Ht, Cui Yh, Liu Z. Oncol Rep. 2009 Nov;22(5):1189-95. Let-7A Microrna Functions As A Potential Tumor Suppressor In Human Laryngeal Cancer.
Liu B, Liu F, Bai L, Li Y, Yang G. Mol Biol Rep. 2010 Mar;37(3):1671-6. Doi: 10.1007/S11033-009-9858-Y. Epub 2009 Oct 13. Molecular Cloning, Expression And Subcellular Distribution Of An Alternative Splice Variant Of The Porcine Sirt2 Gene.
Zhang K, Lu Y, Yang P, Li C, Sun H, Tao D, Liu Y, Zhang S, Ma Y. Plos One. 2012;7(7):E41973. Doi: 10.1371/Journal.Pone.0041973. Epub 2012 Jul 27. Hili Inhibits Tgf-?? Signaling By Interacting With Hsp90 And Promoting T??r Degradation.
Hao Hp, Wen Lb, Li Jr, Wang Y, Ni B, Wang R, Wang X, Sun Mx, Fan Hj, Mao X. Antiviral Res. 2015 May;117:99-109. Doi: 10.1016/J.Antiviral.2015.02.010. Epub 2015 Mar 5. Licl Inhibits Prrsv Infection By Enhancing Wnt/??-Catenin Pathway And Suppressin...
Wang H, Huo N, Li F, Fu S, Xue Y, Yang T, Wen X, Ding Y, Duan X. Mol Cell Biochem. 2010 Sep;342(1-2):191-9. Doi: 10.1007/S11010-010-0483-9. Epub 2010 May 16. Osteogenic Role Of Endosomal Chloride Channels In Mc3T3-E1 Cells.
Zhao P, Li Y, Gao G, Wang S, Yan Y, Zhan X, Liu Z, Mao Z, Chen S, Wang L. Eur J Med Chem. 2014 Oct 30;86:165-74. Doi: 10.1016/J.Ejmech.2014.08.049. Epub 2014 Aug 15. Design, Synthesis And Biological Evaluation Of N-Alkyl Or Aryl Substituted Isoind...
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...
Yuan Y, Zhang J, Cai L, Ding C, Wang X, Chen H, Wang X, Yan J, Lu J. Oncol Rep. 2013 Jun;29(6):2291-6. Doi: 10.3892/Or.2013.2390. Epub 2013 Apr 8. Leptin Induces Cell Proliferation And Reduces Cell Apoptosis By Activating C-Myc In Cervical Cancer.
Qin Xg, Hua Z, Shuang W, Wang Yh, Cui Yd. Pharm Biol. 2010 Mar;48(3):275-81. Doi: 10.3109/13880200903104101. Effects Of Matrine On Hepg2 Cell Proliferation And Expression Of Tumor Relevant Proteins In Vitro.
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to c myc antibody, myc antibody, c myc tag antibody
Write a review for PA1555
Copyright © 2019 Boster Biological Technology Inc. All rights reserved.