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Product Info Summary
| SKU: | PA1555 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-c-Myc Antibody Picoband®
SKU/Catalog Number
PA1555
BA1284-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-c-Myc Antibody catalog # PA1555. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Myc Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1555)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human c-Myc, different from the related mouse and rat sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1555 is reactive to MYC in Human, Mouse, Rat
Observed Molecular Weight
49 kDa
Calculated molecular weight
48.8 kDa
Background of MYC
C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1555 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: HELA Whole Cell, HEPG2 Whole Cell
Validation Images & Assay Conditions
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Anti-c-Myc antibody, PA1555, Western blotting
All lanes: Anti c-Myc(PA1555) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
Click image to see more details
CTHRC1 knockdown inhibited the Wnt/β-catenin pathway. Western blotting was used to analyze the β-catenin, Cyclin D1, and c-myc expression in CTHRC1 knockdown and control group. ***P < 0.001 . Download full-size image DOI:
Index in PubMed under a CC BY license. PMID: 37273536
Specific Publications For Anti-c-Myc Antibody Picoband® (PA1555)
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4 Customer Q&As for Anti-c-Myc Antibody Picoband®
Question
We are currently using anti-c-Myc antibody PA1555 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The anti-c-Myc antibody (PA1555) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-24
Question
My boss were satisfied with the WB result of your anti-c-Myc antibody. However we have been able to see positive staining in thoracic mammary gland nucleoplasm using this antibody. Is that expected? Could you tell me where is MYC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
Based on literature, thoracic mammary gland does express MYC. Generally MYC expresses in nucleus, nucleoplasm. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-01-04
Question
We have seen staining in rat cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-c-Myc antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
From literature cervix carcinoma erythroleukemia does express MYC. From Uniprot.org, MYC is expressed in thoracic mammary gland, uterus, cervix, placenta testis, promyelocytic leukemia, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-10-06
Question
My lab would like using your anti-c-Myc antibody for transcription of e2f targets under negative control by dream complex studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
K. Wu
Verified customer
Asked: 2016-09-30
Answer
Thanks for your inquiry. This PA1555 anti-c-Myc antibody is tested on hela whole cell lysate, hepg2 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-09-30
