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Product Info Summary
| SKU: | M03047 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Calbindin CALB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03047
BM4988 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Calbindin CALB1 Rabbit Monoclonal Antibody catalog # M03047. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calbindin CALB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03047)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAAA-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Calbindin
Reactive Species
M03047 is reactive to CALB1 in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
30.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03047 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of Calbindin expression in human fetal brain lysate.
Specific Publications For Anti-Calbindin CALB1 Rabbit Monoclonal Antibody (M03047)
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Customer Q&As
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15 Customer Q&As for Anti-Calbindin CALB1 Rabbit Monoclonal Antibody
Question
We are currently using anti-Calbindin Rabbit Monoclonal antibody M03047 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
The anti-Calbindin Rabbit Monoclonal antibody (M03047) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-21
Question
I was wanting to use your anti-Calbindin Rabbit Monoclonal antibody for WB for human amygdala on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human amygdala identification?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
It shows on the product datasheet, M03047 anti-Calbindin Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human amygdala in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-01
Question
Is this M03047 anti-Calbindin Rabbit Monoclonal antibody reactive to the isotypes of CALB1?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
The immunogen of M03047 anti-Calbindin Rabbit Monoclonal antibody is A synthesized peptide derived from human Calbindin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-30
Question
Will M03047 anti-Calbindin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
You can see on the product datasheet, M03047 anti-Calbindin Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala using anti-Calbindin Rabbit Monoclonal antibody M03047. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-04-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-23
Question
Would anti-Calbindin Rabbit Monoclonal antibody M03047 work for WB with amygdala?
Verified Customer
Verified customer
Asked: 2019-04-02
Answer
According to the expression profile of amygdala, CALB1 is highly expressed in amygdala. So, it is likely that anti-Calbindin Rabbit Monoclonal antibody M03047 will work for WB with amygdala.
Boster Scientific Support
Answered: 2019-04-02
Question
Which Calbindin antibody would you recommend for WB on human samples and a replacment to Abcam’s Ab108404?
Verified customer
Asked: 2019-03-27
Answer
The Anti-Calbindin CALB1 Rabbit Monoclonal Antibody (M03047) is the most suitable replacement for the attached abcam antibody.
Boster Scientific Support
Answered: 2019-03-27
Question
We have observed staining in human caudate nucleus. Any tips? Is anti-Calbindin Rabbit Monoclonal antibody supposed to stain caudate nucleus positively?
Verified Customer
Verified customer
Asked: 2019-03-19
Answer
From literature caudate nucleus does express CALB1. From Uniprot.org, CALB1 is expressed in caudate nucleus, brain, amygdala, cerebellum substantia nigra, kidney lung, among other tissues. Regarding which tissues have CALB1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cerebellum, and Substantia nigra, Pubmed ID: 14702039
Brain, Pubmed ID: 2565876, 3691519
Kidney, and Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-03-19
Question
Is a blocking peptide available for product anti-Calbindin Rabbit Monoclonal antibody (M03047)?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
We do provide the blocking peptide for product anti-Calbindin Rabbit Monoclonal antibody (M03047). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-22
Question
Our lab used your anti-Calbindin Rabbit Monoclonal antibody for WB on caudate nucleus a few months ago. I am using human, and We want to use the antibody for IF next. Our lab want to know about examining caudate nucleus as well as cerebellum substantia nigra in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
I have checked the website and datasheets of our anti-Calbindin Rabbit Monoclonal antibody and I see that M03047 has been tested on human in both WB and IF. Thus M03047 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-07-03
Question
Please see the WB image, lot number and protocol we used for amygdala using anti-Calbindin Rabbit Monoclonal antibody M03047. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-24
Question
I have a question about product M03047, anti-Calbindin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03047 anti-Calbindin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-19
Question
Is there a BSA free version of anti-Calbindin Rabbit Monoclonal antibody M03047 available?
H. Williams
Verified customer
Asked: 2016-11-07
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Calbindin Rabbit Monoclonal antibody M03047 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-11-07
Question
My question regards to test anti-Calbindin Rabbit Monoclonal antibody M03047 on human amygdala for research purposes, then I may be interested in using anti-Calbindin Rabbit Monoclonal antibody M03047 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Zhao
Verified customer
Asked: 2015-04-08
Answer
The products we sell, including anti-Calbindin Rabbit Monoclonal antibody M03047, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-04-08
Question
I see that the anti-Calbindin Rabbit Monoclonal antibody M03047 works with WB, what is the protocol used to produce the result images on the product page?
R. Parker
Verified customer
Asked: 2014-08-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-08-22
