Click image to see more details
Product Info Summary
| SKU: | A11515 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CaMK1-beta PNCK Antibody
SKU/Catalog Number
A11515
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CaMK1-beta PNCK Antibody (Catalog# A11515). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CaMK1-beta PNCK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11515)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CaMK1-beta. Immunogen sequence location: 161-210
Reactive Species
A11515 is reactive to PNCK in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
38.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11515 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CaMK1-beta Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from LOVO cells, treated with H2O2 100uM 30', using CaMK1-beta Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CaMK1-beta PNCK Antibody (A11515)
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Customer Q&As
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6 Customer Q&As for Anti-CaMK1-beta PNCK Antibody
Question
We are currently using anti-CaMK1-beta antibody A11515 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
The anti-CaMK1-beta antibody (A11515) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-10
Question
I see that the anti-CaMK1-beta antibody A11515 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-06-05
Question
Does anti-CaMK1-beta antibody A11515 work on horse IHC with brain?
G. Anderson
Verified customer
Asked: 2018-04-24
Answer
Our lab technicians have not validated anti-CaMK1-beta antibody A11515 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CaMK1-beta antibody A11515 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-24
Question
Does A11515 anti-CaMK1-beta antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Walker
Verified customer
Asked: 2017-11-20
Answer
As indicated on the product datasheet, A11515 anti-CaMK1-beta antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver thalamus using anti-CaMK1-beta antibody A11515. Let me know if you need anything else.
N. Evans
Verified customer
Asked: 2016-11-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-11-10
Question
Can you help my question with product A11515, anti-CaMK1-beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Huang
Verified customer
Asked: 2016-10-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11515 anti-CaMK1-beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-10-11
