Overview
| Product Name |
Anti-CaMK1-beta PNCK Antibody
See more PNCK products |
| Catalog# |
A11515 |
| Pack Size |
100ul |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-CaMK1-beta PNCK Antibody catalog # A11515. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. |
| Cite This Product |
Anti-CaMK1-beta PNCK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11515)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human, Mouse, Rat |
| Applications |
IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
A peptide derived from human CaMK1-beta. Immunogen sequence location: 161-210 |
Gene/Protein Basic Information For PNCK (Source: Uniprot.org, NCBI)
| Uniprot Id | Q6P2M8 |
|---|
| NCBI Gene Id | 139728 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | PNCK |
|---|
| Protein Name | Calcium/calmodulin-dependent protein kinase type 1B |
|---|
| Superfamily | protein kinase superfamily |
|---|
| Alternative Names | PNCK|BSTK3; calcium/calmodulin-dependent protein kinase type 1B; CaM kinase I beta; CaM kinase IB; CaMK1b; CaM-KI beta; caMKI-beta; EC 2.7.11; EC 2.7.11.17; FLJ50403; FLJ50549; FLJ56451; FLJ59811; MGC45419; pregnancy up-regulated non-ubiquitously expressed CaM kinase; pregnancy upregulated non-ubiquitously expressed CaM kinase; Pregnancy up-regulated non-ubiquitously-expressed CaM kinase |
|---|
| Molecular Weight | 38500 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PNCK, check out the PNCK Infographic
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Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for PNCK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the PNCK infographic
Our Boster Quality Guarantee for Anti-CaMK1-beta PNCK Antibody covers its use in the following applications.
User need to optimize the dilution ratio for this antibody
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Western blot analysis of lysates from LOVO cells, treated with H2O2 100uM 30', using CaMK1-beta Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CaMK1-beta Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
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Questions and answers from customers related to A11515 Anti-CaMK1-beta PNCK Antibody
6 Related Questions
Question
We are currently using anti-CaMK1-beta antibody A11515 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Asked: 2018-09-10
Answer
The anti-CaMK1-beta antibody (A11515) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-10
Question
I see that the anti-CaMK1-beta antibody A11515 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Asked: 2018-06-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-05
Question
Does anti-CaMK1-beta antibody A11515 work on horse IHC with brain?
G. Anderson
Asked: 2018-04-24
Answer
Our lab technicians have not validated anti-CaMK1-beta antibody A11515 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CaMK1-beta antibody A11515 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-24
Question
Does A11515 anti-CaMK1-beta antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Walker
Asked: 2017-11-20
Answer
As indicated on the product datasheet, A11515 anti-CaMK1-beta antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver thalamus using anti-CaMK1-beta antibody A11515. Let me know if you need anything else.
N. Evans
Asked: 2016-11-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-11-10
Question
Can you help my question with product A11515, anti-CaMK1-beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Huang
Asked: 2016-10-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11515 anti-CaMK1-beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-10-11
