Anti-Neuromodulin GAP43 Antibody

GAP-43 antibody

Boster Bio Anti-Neuromodulin GAP43 Antibody catalog # PA1037. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 9 publication(s).

Product Info Summary

SKU: PA1037
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Neuromodulin GAP43 Antibody

View all GAP-43 Antibodies

SKU/Catalog Number

PA1037

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Neuromodulin GAP43 Antibody catalog # PA1037. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Neuromodulin GAP43 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1037)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GAP43, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1037 is reactive to GAP43 in Human, Mouse, Rat

Applications

PA1037 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

24.803kDa

Background of GAP-43

Growth Associated Protein-43 (GAP-43), also known as nerve growth-related growth peptide GAP43, shares a high degree of homology between the sequence of the human gene and the rat gene. GAP-43 is considered a crucial component of an effective regenerative response in the nervous system. Somatic cell hybrids demonstrate localization of the GAP-43 gene to human chromosome 3 and to mouse chromosome 16. GAP-43 has been termed a "growth" or "plasticity" protein because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration. GAP-43 regulates growth of axons and modulates the formation of new connections.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For GAP43 (Source: Uniprot.org, NCBI)

Gene Name

GAP43

Full Name

Neuromodulin

Weight

24.803kDa

Superfamily

neuromodulin family

Alternative Names

Axonal membrane protein GAP-43; B-50; Basp2; calmodulin-binding protein P-57; GAP43; GAP-43; growth associated protein 43; Growth-associated protein 43; nerve growth-related peptide GAP43; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; PP46; protein F1 GAP43 B-50, GAP-43, PP46 growth associated protein 43 neuromodulin|axonal membrane protein GAP-43|calmodulin-binding protein P-57|nerve growth-related peptide GAP43|neural phosphoprotein B-50|neuron growth-associated protein 43|protein F1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GAP43, check out the GAP43 Infographic

GAP43 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAP43: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1037 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Fructus Corni extract-induced neuritogenesis in PC12 cells is associated with the suppression of stromal interaction molecule 1 expression and inhibition of Ca2+ influx

Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats

Liu Z, Liu H, Zeng ZH. Biomed Res Int. 2018 Mar 7;2018:8697913. doi: 10.1155/2018/8697913. eCollection 2018. Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Incre...

Liu H, Lv P, Zhu Y, Wu H, Zhang K, Xu F, Zheng L, Zhao J. Sci Rep. 2017 Jan 5;7:39869. doi: 10.1038/srep39869. Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo

Yuan Q, Sun L, Yu H, An C, Biosci Biotechnol Biochem. 2017 Apr 10:1-8. doi: 10.1080/09168451.2017.1313695. Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system.

Zou H, Ding Y, Shi W, Xu X, Gong A, Zhang Z, Liu J. Cell Mol Neurobiol. 2015 Apr;35(3):313-22. Doi: 10.1007/S10571-014-0126-X. Epub 2014 Oct 29. Microrna-29C/Pten Pathway Is Involved In Mice Brain Development And Modulates Neurite Outgrowth In Pc1...

Zou H, Ding Y, Wang K, Xiong E, Peng W, Du F, Zhang Z, Liu J, Gong A. Neuroscience. 2015 Apr 16;291:289-300. Doi: 10.1016/J.Neuroscience.2015.01.055. Epub 2015 Feb 7. Microrna-29A/Pten Pathway Modulates Neurite Outgrowth In Pc12 Cells.

Nie X, Deng M, Yang M, Liu L, Zhang Y, Wen X. Cell Biochem Biophys. 2014 Jan;68(1):163-72. Doi: 10.1007/S12013-013-9683-8. Axonal Regeneration And Remyelination Evaluation Of Chitosan/Gelatin-Based Nerve Guide Combined With Transforming Growth Fac...

Chen Y, Wei G, Nie H, Lin Y, Tian H, Liu Y, Yu X, Cheng S, Yan R, Wang Q, Liu Dh, Deng W, Lai Y, Zhou Jh, Zhang Sx, Lin Ww, Chen Df. Brain Res. 2014 Mar 13;1552:41-54. Doi: 10.1016/J.Brainres.2014.01.005. Epub 2014 Jan 20. ??-Asarone Prevents Auto...

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18 Customer Q&As for Anti-Neuromodulin GAP43 Antibody

Question

I have a question about product PA1037, anti-GAP43 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1037 anti-GAP43 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-16

Question

My colleagues were well pleased with the WB result of your anti-GAP43 antibody. However we have observed positive staining in brain cortex subthalamic nucleus cell membrane using this antibody. Is that expected? Could you tell me where is GAP43 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

According to literature, brain cortex subthalamic nucleus does express GAP43. Generally GAP43 expresses in cell membrane. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-01-27

Question

We ordered your anti-GAP43 antibody for IHC on anterior cingulate cortex in the past. I am using rat, and We want to use the antibody for WB next. I am interested in examining anterior cingulate cortex as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

I took a look at the website and datasheets of our anti-GAP43 antibody and it appears that PA1037 has been tested on rat in both IHC and WB. Thus PA1037 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-12-11

Question

Is this PA1037 anti-GAP43 antibody reactive to the isotypes of GAP43?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

The immunogen of PA1037 anti-GAP43 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human GAP43(216-238aa KPKESARQDEGKEEEPEADQEHA), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-22

Question

We were wondering how large is your storage of the current batch? If we were to order this product again in two years, would we get antibodies from the same batch?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

We have 10*100ug PA1037 in stock. How many PA1037 does the customer want? We can manufacture a new batch and provide the antibodies from the same batch in two years. It takes around four months to manufacture a new batch.

Boster Scientific Support

Answered: 2019-09-27

Question

Is it possible to provide PA1037 in a buffer without BSA?

Verified customer

Asked: 2019-09-23

Answer

It is not certain if the BSA could be removed completely from a current batch. It is better to produce a new batch without BSA.

Boster Scientific Support

Answered: 2019-09-23

Question

Is a blocking peptide available for product anti-GAP43 antibody (PA1037)?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

We do provide the blocking peptide for product anti-GAP43 antibody (PA1037). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-11

Question

I see that the anti-GAP43 antibody PA1037 works with WB, what is the protocol used to produce the result images on the product page?

B. Miller

Verified customer

Asked: 2019-04-10

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-04-10

Question

Would PA1037 anti-GAP43 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-06-07

Answer

You can see on the product datasheet, PA1037 anti-GAP43 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-06-07

Question

We are currently using anti-GAP43 antibody PA1037 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-05-18

Answer

The anti-GAP43 antibody (PA1037) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-18

Question

Will anti-GAP43 antibody PA1037 work for WB with anterior cingulate cortex?

Verified Customer

Verified customer

Asked: 2018-04-13

Answer

According to the expression profile of anterior cingulate cortex, GAP43 is highly expressed in anterior cingulate cortex. So, it is likely that anti-GAP43 antibody PA1037 will work for WB with anterior cingulate cortex.

Boster Scientific Support

Answered: 2018-04-13

Question

My lab would like to test anti-GAP43 antibody PA1037 on mouse anterior cingulate cortex for research purposes, then I may be interested in using anti-GAP43 antibody PA1037 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

E. Roberts

Verified customer

Asked: 2018-02-22

Answer

The products we sell, including anti-GAP43 antibody PA1037, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-02-22

Question

We have been able to see staining in mouse brain cortex subthalamic nucleus. Do you have any suggestions? Is anti-GAP43 antibody supposed to stain brain cortex subthalamic nucleus positively?

J. Gonzalez

Verified customer

Asked: 2017-12-01

Answer

From literature brain cortex subthalamic nucleus does express GAP43. From Uniprot.org, GAP43 is expressed in anterior cingulate cortex, brain cortex subthalamic nucleus, brain, among other tissues. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2017-12-01

Question

We need using your anti-GAP43 antibody for cell fate commitment studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-08-11

Answer

I appreciate your inquiry. This PA1037 anti-GAP43 antibody is tested on u87 whole cell lysate, rat brain tissue, tissue lysate, mouse brain, human meningeoma tissue, glioma tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-08-11

Question

Is there a BSA free version of anti-GAP43 antibody PA1037 available?

Verified Customer

Verified customer

Asked: 2017-07-12

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GAP43 antibody PA1037 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-07-12

Question

Here is the WB image, lot number and protocol we used for anterior cingulate cortex using anti-GAP43 antibody PA1037. Please let me know if you require anything else.

L. Gonzalez

Verified customer

Asked: 2016-07-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-07-13

Question

I was wanting to use your anti-GAP43 antibody for WB for mouse anterior cingulate cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse anterior cingulate cortex identification?

D. Zhang

Verified customer

Asked: 2016-05-27

Answer

As indicated on the product datasheet, PA1037 anti-GAP43 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-05-27

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-GAP43 antibody PA1037. Let me know if you need anything else.

J. Gonzalez

Verified customer

Asked: 2015-03-10

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-03-10

Order DetailsPrice
PA1037

100μg

$370
PA1037-10ug

10μg sample (liquid)

$99
PA1037-Biotin

100 μg Biotin conjugated

$570
PA1037-Cy3

100 μg Cy3 conjugated

$570
PA1037-Dylight488

100 μg Dylight488 conjugated

$570
PA1037-Dylight550

100 μg Dylight550 conjugated

$570
PA1037-Dylight594

100 μg Dylight594 conjugated

$570
PA1037-FITC

100 μg FITC conjugated

$570
PA1037-HRP

100 μg HRP conjugated

$570
PA1037-APC

100 μg APC conjugated

$670
PA1037-PE

100 μg PE conjugated

$670
PA1037-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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