|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-GAP43 Antibody|
|Description||Rabbit IgG polyclonal antibody for Neuromodulin(GAP43) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-GAP43 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1037)|
|Replacement Item||This antibody may replace the following items: sc-10786|sc-135697|sc-135915|sc-17109|sc-17109-R|sc-17790|sc-33705|sc-7457|sc-7458 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human GAP43(216-238aa KPKESARQDEGKEEEPEADQEHA), different from the related mouse and rat sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||24803 MW|
|Protein Function||This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. .|
|Sequence Similarities||Belongs to the neuromodulin family.|
|Subcellular Localization||Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, growth cone membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, synapse . Cell projection, filopodium membrane ; Peripheral membrane protein . Cytoplasmic surface of growth cone and synaptic plasma membranes.|
|Alternative Names||Neuromodulin;Axonal membrane protein GAP-43;Growth-associated protein 43;Neural phosphoprotein B-50;pp46;GAP43;|
|Research Areas|||neuroscience|cell type marker|neuron marker|growth cone| neuroscience|synapse marker|neurology process|growth and development|axonal guidance proteins||
Background for Neuromodulin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-GAP43 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-GAP43 Antibody Images
Click the images to enlarge.
All lanes: Anti GAP43 (PA1037) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 43KD
Observed bind size: 43KD
IHC(P): Rat Brain Tissue
IHC(P): Human Glioma Tissue
IHC(P): Human Meningeoma Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,