Anti-Caspase 8/CASP8 Antibody (Monoclonal, 32C79)

Product Name

Anti-Caspase 8/CASP8 Antibody (Monoclonal, 32C79)

View all Casp8 Antibodies

SKU/Catalog Number

M00042-6

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-Caspase 8/CASP8 Antibody (Monoclonal, 32C79) catalog # M00042-6. Tested in WB, ICC/IF applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase 8/CASP8 Antibody (Monoclonal, 32C79) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00042-6)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

32C79

Immunogen

Synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein.

Reactive Species

M00042-6 is reactive to Casp8 in Mouse

Calculated molecular weight

55.4 kDa

Background of Casp8

CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. In addtion, this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00042-6 is guaranteed for IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunocytochemistry/Immunofluorescence, 1:50-200

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information Casp8

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