Anti-Catalase Antibody Picoband™

Boster Bio Anti-Catalase Antibody Picoband™ catalog # PB9925. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9925
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB

Product Name

Anti-Catalase Antibody Picoband™

See all Catalase products

SKU/Catalog Number

PB9925

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Catalase Antibody Picoband™ catalog # PB9925. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Catalase Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9925)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human Catalase recombinant protein (Position: E344-L527). Human Catalase shares 85.3% and 82.6% amino acid (aa) sequence identity with mouse and rat Catalase, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9925 is reactive to CAT in Human, Mouse, Rat

Applications

PB9925 is guaranteed for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CAT (Source: Uniprot.Org, NCBI)

Uniprot ID

P04040

Gene ID

847

Gene Name

CAT

Full Name

Catalase

Weight

59.756kDa

Superfamily

catalase family

Alternative Names

Cas1; CAT; Catalase; Cs-1; EC 1.11.1.6; MGC138422; MGC138424 CAT catalase catalase|epididymis secretory sperm binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CAT, check out the CAT Infographic

CAT infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CAT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PB9925

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Catalase Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Catalase Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-Catalase Antibody Picoband™

Question

My team were happy with the WB result of your anti-Catalase antibody. However we have observed positive staining in liver peroxisome. using this antibody. Is that expected? Could you tell me where is CAT supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

According to literature, liver does express CAT. Generally CAT expresses in peroxisome. Regarding which tissues have CAT expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 2308162
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Erythrocyte, Pubmed ID: 7882369
Eye, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 6548744
Kidney, Pubmed ID: 3755526
Liver, Pubmed ID: 11728823, 24275569
Placenta, and Uterus, Pubmed ID: 14702039
Platelet, Pubmed ID: 12665801, 18088087

Boster Scientific Support

Answered: 2020-01-22

Question

We have been able to see staining in human placenta uterus. Any tips? Is anti-Catalase antibody supposed to stain placenta uterus positively?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

From literature placenta uterus does express CAT. From Uniprot.org, CAT is expressed in bone marrow, kidney, liver, placenta uterus, eye, platelet, erythrocyte, fibroblast, brain cajal-retzius cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CAT expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 2308162
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Erythrocyte, Pubmed ID: 7882369
Eye, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 6548744
Kidney, Pubmed ID: 3755526
Liver, Pubmed ID: 11728823, 24275569
Placenta, and Uterus, Pubmed ID: 14702039
Platelet, Pubmed ID: 12665801, 18088087

Boster Scientific Support

Answered: 2019-12-19

Question

My question regarding product PB9925, anti-Catalase antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9925 anti-Catalase antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-09

Question

My question regards using your anti-Catalase antibody for protein targeting to peroxisome studies. Has this antibody been tested with western blotting on mouse liver? We would like to see some validation images before ordering.

D. Jha

Verified customer

Asked: 2019-08-08

Answer

Thank you for your inquiry. This PB9925 anti-Catalase antibody is validated on mouse liver, a549 whole cell lysates, siha cells. It is guaranteed to work for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-08

Question

Does anti-Catalase antibody PB9925 work for Flow Cytometry with platelet?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

According to the expression profile of platelet, CAT is highly expressed in platelet. So, it is likely that anti-Catalase antibody PB9925 will work for Flow Cytometry with platelet.

Boster Scientific Support

Answered: 2019-08-05

Question

Is a blocking peptide available for product anti-Catalase antibody (PB9925)?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

We do provide the blocking peptide for product anti-Catalase antibody (PB9925). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-26

Question

I was wanting to use to test anti-Catalase antibody PB9925 on rat platelet for research purposes, then I may be interested in using anti-Catalase antibody PB9925 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

The products we sell, including anti-Catalase antibody PB9925, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-17

Question

Would PB9925 anti-Catalase antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-11-23

Answer

As indicated on the product datasheet, PB9925 anti-Catalase antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-11-23

Question

I see that the anti-Catalase antibody PB9925 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-08-23

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-08-23

Question

See attached the WB image, lot number and protocol we used for platelet using anti-Catalase antibody PB9925. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-07-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-24

Question

Is this PB9925 anti-Catalase antibody reactive to the isotypes of CAT?

Verified Customer

Verified customer

Asked: 2017-05-23

Answer

The immunogen of PB9925 anti-Catalase antibody is E. coli-derived human Catalase recombinant protein (Position: E344-L527). Human Catalase shares 85.3% and 82.6% amino acid (aa) sequence identity with mouse and rat Catalase, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-05-23

Question

I was wanting to use your anti-Catalase antibody for Flow Cytometry for rat platelet on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat platelet identification?

P. Wu

Verified customer

Asked: 2016-07-19

Answer

It shows on the product datasheet, PB9925 anti-Catalase antibody has been validated for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat platelet in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-07-19

Question

Do you have a BSA free version of anti-Catalase antibody PB9925 available?

K. Bhatt

Verified customer

Asked: 2015-01-21

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Catalase antibody PB9925 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-01-21

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-Catalase antibody PB9925. Let me know if you need anything else.

V. Li

Verified customer

Asked: 2014-12-22

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-12-22

Question

We ordered your anti-Catalase antibody for IHC-F on liver in a previous project. I am using human, and I plan to use the antibody for WB next. We want examining liver as well as kidney in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

J. Krishna

Verified customer

Asked: 2014-08-14

Answer

I took a look at the website and datasheets of our anti-Catalase antibody and it appears that PB9925 has been validated on human in both IHC-F and WB. Thus PB9925 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-08-14

Question

We are currently using anti-Catalase antibody PB9925 for mouse tissue, and we are well pleased with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

K. Li

Verified customer

Asked: 2014-03-28

Answer

The anti-Catalase antibody (PB9925) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-03-28

Conjugation

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
PB9925
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.