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- Table of Contents
CAT antibody (PB9925) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Flow Cytometry analysis of Jurkat cells using anti-Catalase antibody (PB9925).
Overlay histogram showing Jurkat cells stained with PB9925 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Catalase Antibody (PB9925, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Jurkat cells stained with PB9925 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Catalase Antibody (PB9925, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Catalase Antibody Picoband®
CAT antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
CAT antibody was positively detected by WB in:
human Hela whole cell, human Jurkat whole cell, human Raji whole cell, human HepG2 whole cell, rat liver tissue, rat PC-12 whole cell, mouse liver tissue, mouse 3T3-L1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CAT antibody was positively detected by IHC in:
human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CAT antibody was positively detected by ICC/IF in:
U2OS cell
CAT antibody was positively detected by FC in:
Jurkat cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Catalase Antibody Picoband®
View all CAT Antibodies
View all CAT Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Catalase recombinant protein (Position: E344-L527). Human Catalase shares 85.3% and 82.6% amino acid (aa) sequence identity with mouse and rat Catalase, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
59.8 kDa
Background of CAT
Catalase is a key antioxidant enzyme in the bodies defensing against oxidative stress. It is also a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene.
Cite This Product
Anti-Catalase Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9925)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
96%
High Homology
Cow
86%
Moderate Homology
Sheep
86%
Moderate Homology
Mouse
85%
Moderate Homology
Rabbit
85%
Moderate Homology
Dog
85%
Moderate Homology
Rat
83%
Moderate Homology
Zebrafish
67%
Low homology
Drosophila
48%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CAT antibody
Immunohistochemistry (IHC) protocol for CAT antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CAT antibody
Flow Cytometry (FC) protocol for CAT antibody
Publications
Application:
Species:
Zika virus infection induces ultrastructural alterations, mitochondrial dysfunction and oxidative stress in human trophoblast HTR-8/SVneo
Published: 2026-04-10
Journal: Frontiers in Cellular and Infection Microbiology
Application: IF
Impact Factor: 4.80
Species: Human
PMID: 42039750
NUDT7 Modulates the UBA52-SREBF1 Signaling Axis to Promote PRRSV Replication via Lipid Synthesis
Published: 2026-01-14
Journal: International Journal of Biological Sciences
Application: WB
Impact Factor: 10.00
Species: Monkey
PMID: 41608635
Mechanisms of Dangguiliuhuang Tang in Streptozotocin-Induced Type 2 Diabetes Mellitus Mice Based on Network Pharmacology, Gut Microbiota, and Experimental Validation
Published: 2025-10-21
Journal: Diabetes Metabolic Syndrome and Obesity-Targets and Therapy
Application: WB
Impact Factor: 3.00
Species: Mouse
PMID: 41146733
Neuroprotective effects of salidroside against 6-OHDA-induced oxidative stress in PC12 cells
Published: 2023-06-22
Journal: NEW JOURNAL OF CHEMISTRY
Application: WB
Impact Factor: 3.30
Species: Rat
Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis
Published: 2022-09-07
Journal: MOLECULAR NUTRITION & FOOD RESEARCH
Application: WB
Impact Factor: 6.58
Species: Mouse
PMID: 36069237
Senescence marker protein30 protects lens epithelial cells against oxidative damage by restoring mitochondrial function
Published: 2022-05-26
Journal: Bioengineered
Application: WB
Impact Factor: 6.83
Species: Human
PMID: 35615975
Cordycepin alleviated metabolic inflammation in Western diet-fed mice by targeting intestinal barrier integrity and intestinal flora
Published: 2022-03-25
Journal: PHARMACOLOGICAL RESEARCH
Application: WB
Impact Factor: 10.33
Species: Mouse
PMID: 35346845
Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis
Published: 2020-06-12
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: IHC,WB
Impact Factor: 4.55
Species: Pig
PMID: 32535382
Sinomenine attenuates renal fibrosis through Nrf2-mediated inhibition of oxidative stress and TGFβ signaling
Published: 2016-05-17
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY
Application: WB
Impact Factor: 3.85
Species: Mouse
PMID: 27211841
Review
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1 Review For Anti-Catalase Antibody Picoband®
This antibody is highly specific and efficient, suitable for Western blot detection of Catalase protein in MARC-145 cells, with only minor nonspecific bands.
| SKU | PB9925 |
|---|---|
| Application | Western Blot |
| Sample | MARC-145 cells |
| Sample Processing Description | Cells or tissues were lysed in RIPA buffer supplemented with protease inhibitor PMSF (100:1) for 10 minutes. The lysate was centrifuged at 12,000 rpm for 15 minutes, and the supernatant was collected. Samples were mixed with 5× loading buffer and denatured at 100 °C for 10 minutes before loading onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | Catalase Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | Anti-rabbit IgG secondary antibody conjugated with horseradish peroxidase (HRP) |
| Secondary Incubation | 1:10000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows a schematic representation of Western blot results for the target protein Catalase and the loading control Actin in MARC-145 cells under normal and post-infection conditions. The target bands are clear and well-defined, and the experimental results are satisfactory. |
Yuchao Yan, Tianjin University
Verified customer
Submitted 2025-12-25
Product Promise
We promise Anti-Catalase Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9925
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026