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- Table of Contents
BSG antibody (PA1526) performs well in IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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IHC analysis of CD147/BSG using anti-CD147/BSG antibody (PA1526).
CD147/BSG was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD147/BSG Antibody (PA1526) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
CD147/BSG was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD147/BSG Antibody (PA1526) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-CD147/BSG Antibody Picoband®
BSG antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC, WB |
Tested Applications
BSG antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
BSG antibody was positively detected by IHC in:
human placenta tissue, human liver cancer tissue, human endometrial carcinoma tissue, human ovarian serous adenocarcinoma tissue, human lung adenocarcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-CD147/BSG Antibody Picoband®
View all BSG Antibodies
View all BSG Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CD147.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35-60 kDa
Calculated Molecular Weight
42.2 kDa
Background of BSG
Emmprin, extracellular matrix metalloproteinase inducer, also known as Emmprin (BSG) or cluster of differentiation 147 (CD147) is a protein that in humans is encoded by the Emmprin gene. the BSG gene contains 8 exons and spans 10.8 kb. The human BSG gene is mapped to 19p13.3. This protein is a determinant for the Ok blood group system. Emmprin has been shown to be an essential receptor on red blood cells for the malaria parasite. Emmprin is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, Emmprin is though also to play a role in intercellular recognition. Emmprin also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. Emmprin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.
Cite This Product
Anti-CD147/BSG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1526)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
78%
Moderate Homology
Dog
67%
Low homology
Cow
61%
Low homology
Sheep
61%
Low homology
Mouse
56%
Low homology
Rat
50%
Low homology
Rabbit
50%
Low homology
Drosophila
39%
Low homology
Zebrafish
28%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for BSG antibody
Immunohistochemistry (IHC) protocol for BSG antibody
Publications
Application:
Overexpression of CD147 is associated with poor prognosis, tumor cell migration and ERK signaling pathway activation in hepatocellular carcinoma
PMID: 28962206
iTRAQ-coupled 2D LC/MS-MS analysis of CXCR7-transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers
PMID: 28927140
Lu Q, Lv G, Kim A, Ha Jm, Kim S. Oncol Lett. 2013 Jan;5(1):201-207. Epub 2012 Oct 19. Expression And Clinical Significance Of Extracellular Matrix Metalloproteinase Inducer, Emmprin/Cd147, In Human Osteosarcoma.
PMID: 23255920
Ying Hz, Liu Yh, Yu B, Wang Zy, Zang Jn, Yu Ch. Food Chem Toxicol. 2013 Feb;52:53-60. Doi: 10.1016/J.Fct.2012.10.030. Epub 2012 Nov 2. Dietary Quercetin Ameliorates Nonalcoholic Steatohepatitis Induced By A High-Fat Diet In Gerbils.
PMID: 23123425
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Product Promise
We promise Anti-CD147/BSG Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1526
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026