Anti-CD147/Emmprin/BSG Antibody Picoband™

BSG antibody

Boster Bio Anti-CD147/Emmprin/BSG Antibody Picoband™ catalog # A00248-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. Cited in 4 publication(s). Independently reviewed in 2 review(s).

Product Info Summary

SKU: A00248-1
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-CD147/Emmprin/BSG Antibody Picoband™

View all BSG Antibodies

SKU/Catalog Number

A00248-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD147/Emmprin/BSG Antibody Picoband™ catalog # A00248-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD147/Emmprin/BSG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00248-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CD147/Emmprin recombinant protein (Position: E138-A323). Human CD147/Emmprin shares 51.1% and 51.9% amino acid (aa) sequence identity with mouse and rat CD147/Emmprin, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A00248-1 is reactive to BSG in Human

Applications

A00248-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

38-55 kDa

Calculated molecular weight

42.2kDa

Background of BSG

Emmprin, extracellular matrix metalloproteinase inducer, also known as Emmprin (BSG) or cluster of differentiation 147 (CD147) is a protein that in humans is encoded by the Emmprin gene. The human BSG gene is mapped to 19p13.3. This protein is a determinant for the Ok blood group system. BSG has been shown to be an essential receptor on red blood cells for the malaria parasite. It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily, it plays fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development. BSG is thought also to play a role in intercellular recognition. It also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. BSG is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For BSG (Source: Uniprot.org, NCBI)

Gene Name

BSG

Full Name

Basigin

Weight

42.2kDa

Alternative Names

5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor BSG 5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, SLC7A11, TCSF basigin (Ok blood group) basigin|OK blood group antigen|collagenase stimulatory factor|extracellular matrix metalloproteinase inducer|hepatoma-associated antigen|leukocyte activation antigen M6|tumor cell-derived collagenase stimulatory factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BSG, check out the BSG Infographic

BSG infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BSG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00248-1 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Overexpression of CD147 is associated with poor prognosis, tumor cell migration and ERK signaling pathway activation in hepatocellular carcinoma

iTRAQ-coupled 2D LC/MS-MS analysis of CXCR7-transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers

Lu Q, Lv G, Kim A, Ha Jm, Kim S. Oncol Lett. 2013 Jan;5(1):201-207. Epub 2012 Oct 19. Expression And Clinical Significance Of Extracellular Matrix Metalloproteinase Inducer, Emmprin/Cd147, In Human Osteosarcoma.

Ying Hz, Liu Yh, Yu B, Wang Zy, Zang Jn, Yu Ch. Food Chem Toxicol. 2013 Feb;52:53-60. Doi: 10.1016/J.Fct.2012.10.030. Epub 2012 Nov 2. Dietary Quercetin Ameliorates Nonalcoholic Steatohepatitis Induced By A High-Fat Diet In Gerbils.

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2 Reviews For Anti-CD147/Emmprin/BSG Antibody Picoband™

0

Immunohistochemistry review for CD147 Emmprin BSG antibody

Excellent

Application Immunohistochemistry (paraffin embedded)
Blocking step 5% BSA as blocking agent for 30 min at 37°C
Sample Prostate cancer
Fixative Fixed with 4% paraformaldehyde
Primary Incubation 4°C overnight
Primary Incubation diluent 5% BSA in TBS
Primary Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Dilution The kit was ready to use, no dilution needed
Secondary Incubation at 37°C for 30 min

Verified customer

Submitted

0

Immunohistochemistry Review For Anti-CD147 Emmprin BSG Antibody

Excellent

Application Immunohistochemistry (paraffin embedded)
Blocking step 5% BSA as blocking agent for 30 min at 37°C
Sample Placenta
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Verified customer

Submitted

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Order DetailsPrice
A00248-1

100μg

$370
A00248-1-10ug

10μg sample (liquid)

$99
A00248-1-Biotin

100 μg Biotin conjugated

$570
A00248-1-Cy3

100 μg Cy3 conjugated

$570
A00248-1-Dylight488

100 μg Dylight488 conjugated

$570
A00248-1-Dylight550

100 μg Dylight550 conjugated

$570
A00248-1-Dylight594

100 μg Dylight594 conjugated

$570
A00248-1-FITC

100 μg FITC conjugated

$570
A00248-1-HRP

100 μg HRP conjugated

$570
A00248-1-APC

100 μg APC conjugated

$670
A00248-1-PE

100 μg PE conjugated

$670
A00248-1-carrier-free

Carrier Free

$370

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A00248-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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