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- Table of Contents
BSG antibody (A00248-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of A549 cells using anti-CD147/Emmprin antibody (A00248-1).
Overlay histogram showing A549 cells stained with A00248-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD147/Emmprin Antibody (A00248-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing A549 cells stained with A00248-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD147/Emmprin Antibody (A00248-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-CD147/Emmprin/BSG Antibody Picoband®
BSG antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
BSG antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
BSG antibody was positively detected by IHC in:
human lung cancer tissue, human tonsil tissue, human intestinal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
BSG antibody was positively detected by ICC/IF in:
A549 cell, A549 cell, HELA cell
BSG antibody was positively detected by IF in:
human intestinal cancer tissue
BSG antibody was positively detected by FC in:
A549 cell, Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Immunofluorescence
2μg/ml
Human
Flow Cytometry
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-CD147/Emmprin/BSG Antibody Picoband®
View all BSG Antibodies
View all BSG Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD147/Emmprin recombinant protein (Position: E138-A323). Human CD147/Emmprin shares 51.1% and 51.9% amino acid (aa) sequence identity with mouse and rat CD147/Emmprin, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38-55 kDa
Calculated Molecular Weight
42.2 kDa
Background of BSG
Emmprin, extracellular matrix metalloproteinase inducer, also known as Emmprin (BSG) or cluster of differentiation 147 (CD147) is a protein that in humans is encoded by the Emmprin gene. The human BSG gene is mapped to 19p13.3. This protein is a determinant for the Ok blood group system. BSG has been shown to be an essential receptor on red blood cells for the malaria parasite. It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily, it plays fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development. BSG is thought also to play a role in intercellular recognition. It also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. BSG is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.
Cite This Product
Anti-CD147/Emmprin/BSG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00248-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
80%
Moderate Homology
Rabbit
58%
Low homology
Dog
58%
Low homology
Cow
57%
Low homology
Sheep
57%
Low homology
Rat
53%
Low homology
Mouse
52%
Low homology
Zebrafish
35%
Low homology
Drosophila
26%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for BSG antibody
Immunohistochemistry (IHC) protocol for BSG antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for BSG antibody
Immunofluorescence (IF) protocol for BSG antibody
Flow Cytometry (FC) protocol for BSG antibody
Publications
Application:
Species:
Thrombo-pathologic features and prognosis of acute ischemic stroke patients treated with remedial stent implantation
Published: 2025-09-16
Journal: Frontiers in Neurology
Application: IHC
Impact Factor: 2.80
Species: Human
PMID: 41036279
Review
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2 Reviews For Anti-CD147/Emmprin/BSG Antibody Picoband®
Immunohistochemistry review for CD147 Emmprin BSG antibody
| SKU | A00248-1 |
|---|---|
| Application | Immunohistochemistry (paraffin embedded) |
| Blocking step | 5% BSA as blocking agent for 30 min at 37°C |
| Sample | Prostate cancer |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Incubation | 4°C overnight |
| Primary Incubation diluent | 5% BSA in TBS |
| Primary Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Dilution | The kit was ready to use, no dilution needed |
| Secondary Incubation | at 37°C for 30 min |
E. Taylor
Verified customer
Submitted 2020-07-22
Immunohistochemistry Review For Anti-CD147 Emmprin BSG Antibody
| SKU | A00248-1 |
|---|---|
| Application | Immunohistochemistry (paraffin embedded) |
| Blocking step | 5% BSA as blocking agent for 30 min at 37°C |
| Sample | Placenta |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 4°C overnight |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
Verified customer
Submitted 2020-04-13
Product Promise
We promise Anti-CD147/Emmprin/BSG Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00248-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026