Anti-CD156c ADAM10 Antibody

Boster Bio Anti-CD156c ADAM10 Antibody catalog # A00566. Tested in IHC, WB applications. This antibody reacts with Rat.

Product Info Summary

SKU: A00566
Size: 100μl
Reactive Species: Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CD156c ADAM10 Antibody

See all ADAM10 products

SKU/Catalog Number

A00566

Size

100μl

Form

Liquid

Description

Boster Bio Anti-CD156c ADAM10 Antibody catalog # A00566. Tested in IHC, WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD156c ADAM10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00566)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human CD156c.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00566 is reactive to ADAM10 in Rat

Applications

A00566 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC-P, 1:100-1:300

Validation Images & Assay Conditions

Gene/Protein Information For ADAM10 (Source: Uniprot.Org, NCBI)

Uniprot ID

O14672

Gene ID

102

Gene Name

ADAM10

Full Name

Disintegrin and metalloproteinase domain-containing protein 10

Weight

84.142kDa

Alternative Names

a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease ADAM10 AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz ADAM metallopeptidase domain 10 disintegrin and metalloproteinase domain-containing protein 10|a disintegrin and metalloprotease domain 10|kuzbanian protein homolog|mammalian disintegrin-metalloprotease

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADAM10, check out the ADAM10 Infographic

ADAM10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ADAM10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A00566

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16 Customer Q&As for Anti-CD156c ADAM10 Antibody

Question

Is there a BSA free version of anti-CD156c antibody A00566 available?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD156c antibody A00566 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-24

Question

Will A00566 anti-CD156c antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

You can see on the product datasheet, A00566 anti-CD156c antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-12

Question

Will anti-CD156c antibody A00566 work on monkey IHC with cervix carcinoma?

R. Li

Verified customer

Asked: 2020-03-11

Answer

Our lab technicians have not validated anti-CD156c antibody A00566 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CD156c antibody A00566 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-11

Question

I was wanting to use your anti-CD156c antibody for IHC for rat leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukemic t-cell identification?

M. Singh

Verified customer

Asked: 2020-02-14

Answer

As indicated on the product datasheet, A00566 anti-CD156c antibody has been tested for IHC, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-14

Question

Is this A00566 anti-CD156c antibody reactive to the isotypes of ADAM10?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

The immunogen of A00566 anti-CD156c antibody is Synthesized peptide derived from the Internal region of human CD156c. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-15

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD156c antibody A00566. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-04

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-04

Question

Would anti-CD156c antibody A00566 work for IHC with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

According to the expression profile of leukemic t-cell, ADAM10 is highly expressed in leukemic t-cell. So, it is likely that anti-CD156c antibody A00566 will work for IHC with leukemic t-cell.

Boster Scientific Support

Answered: 2019-10-11

Question

Is a blocking peptide available for product anti-CD156c antibody (A00566)?

N. Banerjee

Verified customer

Asked: 2019-09-10

Answer

We do provide the blocking peptide for product anti-CD156c antibody (A00566). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-10

Question

We are currently using anti-CD156c antibody A00566 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

The anti-CD156c antibody (A00566) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-15

Question

Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-CD156c antibody A00566. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-12-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-13

Question

I am interested in to test anti-CD156c antibody A00566 on rat leukemic t-cell for research purposes, then I may be interested in using anti-CD156c antibody A00566 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-10-24

Answer

The products we sell, including anti-CD156c antibody A00566, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-10-24

Question

We were happy with the WB result of your anti-CD156c antibody. However we have been able to see positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is ADAM10 supposed to be expressed?

E. Anderson

Verified customer

Asked: 2018-07-09

Answer

According to literature, cervix carcinoma does express ADAM10. Generally ADAM10 expresses in cell membrane. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699

Boster Scientific Support

Answered: 2018-07-09

Question

I see that the anti-CD156c antibody A00566 works with IHC, what is the protocol used to produce the result images on the product page?

W. Jones

Verified customer

Asked: 2015-10-26

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-10-26

Question

We purchased anti-CD156c antibody for IHC on liver in a previous experiment. I am using rat, and I plan to use the antibody for WB next. My question regards examining liver as well as platelet in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

P. Zhang

Verified customer

Asked: 2014-10-15

Answer

I have checked the website and datasheets of our anti-CD156c antibody and it appears that A00566 has been tested on rat in both IHC and WB. Thus A00566 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-10-15

Question

We have been able to see staining in rat platelet. Do you have any suggestions? Is anti-CD156c antibody supposed to stain platelet positively?

N. Singh

Verified customer

Asked: 2014-08-25

Answer

From what I have seen in literature platelet does express ADAM10. From what I have seen in Uniprot.org, ADAM10 is expressed in testis, placenta, platelet, liver, leukemic t-cell, cervix carcinoma, among other tissues. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699

Boster Scientific Support

Answered: 2014-08-25

Question

My question regarding product A00566, anti-CD156c antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

C. Parker

Verified customer

Asked: 2013-06-03

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00566 anti-CD156c antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-06-03

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A00566
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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