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Product Info Summary
| SKU: | A03958 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD177 antigen CD177 Antibody
SKU/Catalog Number
A03958
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD177 antigen CD177 Antibody catalog # A03958. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD177 antigen CD177 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03958)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CD177. AA range:361-410
Reactive Species
A03958 is reactive to CD177 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
46.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03958 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of K562 cells using CD177 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from K562 cells, using CD177 Antibody.
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Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
Specific Publications For Anti-CD177 antigen CD177 Antibody (A03958)
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4 Customer Q&As for Anti-CD177 antigen CD177 Antibody
Question
Would anti-CD177 antibody A03958 work on pig WB with blood?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
Our lab technicians have not tested anti-CD177 antibody A03958 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CD177 antibody A03958 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-20
Question
I see that the anti-CD177 antibody A03958 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-11
Question
Is there a BSA free version of anti-CD177 antibody A03958 available?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD177 antibody A03958 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-22
Question
We are currently using anti-CD177 antibody A03958 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The anti-CD177 antibody (A03958) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-18
