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Product Info Summary
| SKU: | EK0332 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Erythropoietin/EPO ELISA Kit PicoKine®
SKU/Catalog Number
EK0332
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Erythropoietin/EPO ELISA Kit PicoKine® (96 Tests). Quantitate Human EPO in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 1mIU/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Erythropoietin/EPO ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0332)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: A28-R193
Sensitivity
<1mIU/ml
Assay Range
3.12 mIU/ml - 200 mIU/ml
Standard Dilution Instructions
See datasheet of EK0332 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0332 is reactive to EPO in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0332 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of EPO
Erythropoietin (EPO), also known as hematopoietin or hemopoietin, is a glycoprotein cytokine secreted by the kidney in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. It is mapped to 7q22.1. This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0332-CAP | Anti-Human EPO Pre-coated 96-well strip microplate | 1 |
| EK0332-ST | Human EPO Standard | 2 vials, 200 mIU/tube |
| EK0332-DA | Human EPO Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (mIU/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
| O.D. | 0.003 | 0.082 | 0.14 | 0.298 | 0.553 | 0.967 | 1.427 | 2.142 |
Data Example Images
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Human EPO PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect EPO, For serum, dilution ratio is 1:1 and concentration is around 60 mIU/m.
For plasma, dilution ratio is 1:1 and concentration is 3 mIU/m..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Erythropoietin/EPO ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (mIU/ml) | 4 | 23 | 68 | 5 | 24 | 76 |
| Standard deviation | 0.17 | 0.99 | 2.79 | 0.33 | 1.63 | 5.47 |
| CV (%) | 4.2% | 4.3% | 4.1% | 6.6% | 6.8% | 7.2% |
Specific Publications For Human Erythropoietin/EPO ELISA Kit PicoKine® (EK0332)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Human Erythropoietin/EPO ELISA Kit PicoKine®
Question
Hello Boster: I used your product EK0332 to measures EPO levels in human serum but I have new research studies to measure rat or dog plasma. Can this kit still suitable for animal plasma?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
The product EK0332 has been design to detect human EPO and been only tested and validated to human samples. We have not tested this kit for any cross-reactivity with other species.
Boster Scientific Support
Answered: 2019-12-25
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Erythropoietin using the EK0332 Human Erythropoietin Picokine® ELISA Kit?
A. Thompson
Verified customer
Asked: 2019-12-21
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-12-21
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
N. Edwards
Verified customer
Asked: 2019-03-31
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-03-31
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
H. Phillips
Verified customer
Asked: 2016-02-03
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-02-03
