Human Erythropoietin/EPO ELISA Kit PicoKine®

Erythropoietin/EPO ELISA kit for Human

Human Erythropoietin/EPO ELISA Kit PicoKine™ (96 Tests). Quantitate Human EPO in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 1mIU/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0332
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human Erythropoietin/EPO ELISA Kit PicoKine®

View all Erythropoietin/EPO ELISA kits

SKU/Catalog Number

EK0332

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Erythropoietin/EPO ELISA Kit PicoKine™ (96 Tests). Quantitate Human EPO in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 1mIU/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Erythropoietin/EPO ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0332)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: A28-R193

Sensitivity

<1mIU/ml

Assay Range

3.12 mIU/ml - 200 mIU/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0332 is reactive to EPO in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK0332 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Erythropoietin/EPO

Erythropoietin (EPO), also known as hematopoietin or hemopoietin, is a glycoprotein cytokine secreted by the kidney in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. It is mapped to 7q22.1. This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.

Kit Components

Catalog Number Description Quantity
EK0332-CAP Anti-Human EPO Pre-coated 96-well strip microplate 1
EK0332-ST Human EPO Standard 2 vials, 200 mIU/tube
EK0332-DA Human EPO Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (mIU/ml)03.126.2512.52550100200
O.D.0.0490.1760.2360.4150.6711.0971.6572.086

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Erythropoietin/EPO, Dilution ratio of 1:1, concentration in serum is around 60mIU/m, in plasma is around 3mIU/m.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Erythropoietin/EPO ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (mIU/ml)4236852476
Standard deviation0.170.992.790.331.635.47
CV (%)4.2%4.3%4.1%6.6%6.8%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Erythropoietin/EPO in ELISA kits from four different production batches/lots.

LotsLot 1 (mIU/ml)Lot 2 (mIU/ml)Lot 3 (mIU/ml)Lot 4 (mIU/ml)Mean (mIU/ml)Standard DeviationCV (%)
Sample 1444440.000.0%
Sample 223232321220.863.9%
Sample 368737665704.276.1%
*number of samples for each test n=16.

Gene/Protein Information For EPO (Source: Uniprot.Org, NCBI)

Uniprot ID

P01588

Gene ID

2056

Gene Name

EPO

Full Name

Erythropoietin

Weight

21.307kDa

Superfamily

EPO/TPO family

Alternative Names

ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 EPO DBAL, ECYT5, EP, MVCD2 erythropoietin erythropoietin|epoetin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EPO, check out the EPO Infographic

EPO infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0332 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Toll-like receptors 2 and 4 mediate the capacity of mesenchymal stromal cells to support the proliferation and differentiation of CD34⁺ cells.

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4 Customer Q&As for Human Erythropoietin/EPO ELISA Kit PicoKine®

Question

Hello Boster: I used your product EK0332 to measures EPO levels in human serum but I have new research studies to measure rat or dog plasma. Can this kit still suitable for animal plasma?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

The product EK0332 has been design to detect human EPO and been only tested and validated to human samples. We have not tested this kit for any cross-reactivity with other species.

Boster Scientific Support

Answered: 2019-12-25

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Erythropoietin using the EK0332 Human Erythropoietin Picokine® ELISA Kit?

A. Thompson

Verified customer

Asked: 2019-12-21

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-12-21

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

N. Edwards

Verified customer

Asked: 2019-03-31

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-03-31

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

H. Phillips

Verified customer

Asked: 2016-02-03

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-02-03

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