Data & Images
|Product Name||Anti-CD25/IL-2sR alpha Antibody|
|Description||Rabbit IgG polyclonal antibody for Interleukin-2 receptor subunit alpha(IL2RA) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-CD25/IL-2sR alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1747)|
|Replacement Item||This antibody may replace the following items: sc-13946|sc-19628|sc-365511|sc-365912|sc-374034|sc-393326|sc-57297|sc-664|sc-665|sc-666 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human CD25/IL-2sR Alpha (39-57aa MAYKEGTMLNCECKRGFRR).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Interleukin-2 receptor subunit alpha(IL-2 receptor subunit alpha/IL-2-RA/IL-2R subunit alpha/IL2-RA)|
|Molecular Weight||30819 MW|
|Protein Function||Receptor for interleukin-2.|
|Sequence Similarities||Contains 2 Sushi (CCP/SCR) domains.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||Interleukin-2 receptor subunit alpha;IL-2 receptor subunit alpha;IL-2-RA;IL-2R subunit alpha;IL2-RA;TAC antigen;p55;CD25;IL2RA;|
|Research Areas|||immunology|adaptive immunity|t cells| immunology|innate immunity|cytokines|interleukins||
Background for Interleukin-2 receptor subunit alpha(IL-2 receptor subunit alpha/IL-2-RA/IL-2R subunit alpha/IL2-RA)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD25/IL-2sR alpha Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD25/IL-2sR alpha Antibody Images
Click the images to enlarge.
All lanes: Anti CD25/IL-2sR Alpha (PA1747) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Lane 6: PANC Whole Cell Lysate at 40ug
Predicted bind size: 31KD
Observed bind size: 45KD
IHC(P): Human Tonsil Tissue
- Review by Prakash A
--Prakash A, NEUROSCIENCE, WRIGHT STATE UNIVERSITY, GRADUATE STUDENT
Applications Western Blot Sample Jurkat Lysates Detection ECL solution
"Our lab is identifying IL-2 receptor components in non-lymphoid primary cell lines. We use Jurkat (E6-1) cell lines as a positive control for our experiments. Got one single band at the correct size. Very sensitive, can use at low concentration. Great antibody. Works well with whole cell lystes from our cell lines."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,