Click image to see more details
Product Info Summary
| SKU: | PB9055 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Mast Cell Chymase/CMA1 Antibody Picoband®
SKU/Catalog Number
PB9055
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mast Cell Chymase/CMA1 Antibody Picoband® catalog # PB9055. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mast Cell Chymase/CMA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9055)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CMA1 recombinant protein (Position: I22-N247). Human CMA1 shares 75% and 74% amino acid (aa) sequences identity with mouse and rat CMA1, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9055 is reactive to CMA1 in Human, Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
27.3 kDa
Background of CMA1
Chymase is a major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. Chymase is mapped to 14q12. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9055 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Positive Control
WB: human PC-3 whole cell, human HepG2 whole cell, rat liver tissue, mouse HEPA1-6 whole cell,
IHC: human placenta tissue
Validation Images & Assay Conditions
Click image to see more details
IHC analysis of CMA1 using anti-CMA1 antibody (PB9055).
CMA1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CMA1 Antibody (PB9055) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Western blot analysis of CMA1 using anti-CMA1 antibody (PB9055).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse HEPA1-6 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CMA1 antigen affinity purified polyclonal antibody (Catalog # PB9055) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CMA1 at approximately 35KD. The expected band size for CMA1 is at 27KD.
Specific Publications For Anti-Mast Cell Chymase/CMA1 Antibody Picoband® (PB9055)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Mast Cell Chymase/CMA1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Mast Cell Chymase/CMA1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
2 Customer Q&As for Anti-Mast Cell Chymase/CMA1 Antibody Picoband®
Question
We are currently using anti-Mast Cell Chymase/CMA1 antibody PB9055 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
The anti-Mast Cell Chymase/CMA1 antibody (PB9055) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-30
Question
Can PB9055 detect active, inactive (or both) forms of CMA1?
Verified customer
Asked: 2019-06-21
Answer
Unfortunately, we are uncertain if the Anti-Mast Cell Chymase/CMA1 Antibody Picoband™ (PB9055) detect active, inactive (or both) forms of CMA1. The immunogen sequence is I22-N247 which contains active sites. Our lab hasn't perform relevant tests to validate the detection form of the antibody.
Boster Scientific Support
Answered: 2019-06-21
