Anti-CD30/TNFRSF8 Antibody

CD30/TNFRSF8 antibody

Boster Bio Anti-CD30/TNFRSF8 Antibody catalog # RP1029. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: RP1029
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-CD30/TNFRSF8 Antibody

View all CD30/TNFRSF8 Antibodies

SKU/Catalog Number

RP1029

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD30/TNFRSF8 Antibody catalog # RP1029. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD30/TNFRSF8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1029)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CD30 recombinant protein (Position: T322-K595). Human CD30 shares 60% and 57% amino acid (aa) sequences identity with mouse and rat CD30, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1029 is reactive to TNFRSF8 in Human

Applications

RP1029 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

67 kDa

Calculated molecular weight

63.747kDa

Background of CD30/TNFRSF8

CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For TNFRSF8 (Source: Uniprot.org, NCBI)

Gene Name

TNFRSF8

Full Name

Tumor necrosis factor receptor superfamily member 8

Weight

63.747kDa

Superfamily

TNFR8 family

Alternative Names

CD30 antigen; CD30; CD30KI-1; CD30L receptor; cytokine receptor CD30; D1S166EKi-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNFRSF8; tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8 TNFRSF8 CD30, D1S166E, Ki-1 TNF receptor superfamily member 8 tumor necrosis factor receptor superfamily member 8|CD30L receptor|Ki-1 antigen|cytokine receptor CD30|lymphocyte activation antigen CD30

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TNFRSF8, check out the TNFRSF8 Infographic

TNFRSF8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TNFRSF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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15 Customer Q&As for Anti-CD30/TNFRSF8 Antibody

Question

I see that the anti-CD30/TNFRSF8 antibody RP1029 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-03

Question

We are currently using anti-CD30/TNFRSF8 antibody RP1029 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

The anti-CD30/TNFRSF8 antibody (RP1029) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-13

Question

See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-CD30/TNFRSF8 antibody RP1029. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-06

Question

Is this RP1029 anti-CD30/TNFRSF8 antibody reactive to the isotypes of TNFRSF8?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

The immunogen of RP1029 anti-CD30/TNFRSF8 antibody is E.coli-derived human CD30 recombinant protein (Position: T322-K595). Human CD30 shares 60% and 57% amino acid (aa) sequences identity with mouse and rat CD30, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-04

Question

Our lab were happy with the WB result of your anti-CD30/TNFRSF8 antibody. However we have seen positive staining in lymphoid tissue isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is TNFRSF8 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-07-16

Answer

From literature, lymphoid tissue does express TNFRSF8. Generally TNFRSF8 expresses in isoform 1: cell membrane, isoform 2: cytoplasm. Regarding which tissues have TNFRSF8 expression, here are a few articles citing expression in various tissues:
Blood, and Testis, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoid tissue, Pubmed ID: 1310894
Placenta, Pubmed ID: 16710414

Boster Scientific Support

Answered: 2019-07-16

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD30/TNFRSF8 antibody RP1029. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-11

Question

I was wanting to use your anti-CD30/TNFRSF8 antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?

B. Krishna

Verified customer

Asked: 2019-05-22

Answer

As indicated on the product datasheet, RP1029 anti-CD30/TNFRSF8 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-22

Question

Is a blocking peptide available for product anti-CD30/TNFRSF8 antibody (RP1029)?

Verified Customer

Verified customer

Asked: 2018-08-02

Answer

We do provide the blocking peptide for product anti-CD30/TNFRSF8 antibody (RP1029). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-08-02

Question

Does RP1029 anti-CD30/TNFRSF8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

C. Gonzalez

Verified customer

Asked: 2018-07-12

Answer

It shows on the product datasheet, RP1029 anti-CD30/TNFRSF8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-07-12

Question

We have seen staining in human lymphoid tissue. Any tips? Is anti-CD30/TNFRSF8 antibody supposed to stain lymphoid tissue positively?

Verified Customer

Verified customer

Asked: 2018-05-18

Answer

From literature lymphoid tissue does express TNFRSF8. From Uniprot.org, TNFRSF8 is expressed in leukocyte, lymphoid tissue, placenta, blood testis, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have TNFRSF8 expression, here are a few articles citing expression in various tissues:
Blood, and Testis, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoid tissue, Pubmed ID: 1310894
Placenta, Pubmed ID: 16710414

Boster Scientific Support

Answered: 2018-05-18

Question

My question regarding product RP1029, anti-CD30/TNFRSF8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-05-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1029 anti-CD30/TNFRSF8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-05-15

Question

Do you have a BSA free version of anti-CD30/TNFRSF8 antibody RP1029 available?

Verified Customer

Verified customer

Asked: 2017-11-14

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD30/TNFRSF8 antibody RP1029 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-11-14

Question

We want using your anti-CD30/TNFRSF8 antibody for positive regulation of trail biosynthetic process studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-07-20

Answer

We appreciate your inquiry. This RP1029 anti-CD30/TNFRSF8 antibody is tested on hela whole cell lysate, 293t whole cell lysate, jurkat whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-07-20

Question

Does anti-CD30/TNFRSF8 antibody RP1029 work for WB with leukemic t-cell?

S. Krishna

Verified customer

Asked: 2017-03-29

Answer

According to the expression profile of leukemic t-cell, TNFRSF8 is highly expressed in leukemic t-cell. So, it is likely that anti-CD30/TNFRSF8 antibody RP1029 will work for WB with leukemic t-cell.

Boster Scientific Support

Answered: 2017-03-29

Question

My question regards to test anti-CD30/TNFRSF8 antibody RP1029 on human leukemic t-cell for research purposes, then I may be interested in using anti-CD30/TNFRSF8 antibody RP1029 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

D. Carter

Verified customer

Asked: 2017-03-06

Answer

The products we sell, including anti-CD30/TNFRSF8 antibody RP1029, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-03-06

Order DetailsPrice
RP1029

100μg

$370
RP1029-10ug

10μg sample (liquid)

$99
RP1029-Biotin

100 μg Biotin conjugated

$570
RP1029-Cy3

100 μg Cy3 conjugated

$570
RP1029-Dylight488

100 μg Dylight488 conjugated

$570
RP1029-Dylight550

100 μg Dylight550 conjugated

$570
RP1029-Dylight594

100 μg Dylight594 conjugated

$570
RP1029-FITC

100 μg FITC conjugated

$570
RP1029-HRP

100 μg HRP conjugated

$570
RP1029-APC

100 μg APC conjugated

$670
RP1029-PE

100 μg PE conjugated

$670
RP1029-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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