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PECAM1 antibody (PA1950-1) performs well in IF, IHC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 17 Publications

pa1950 1 cd31 primary antibodies if testing 1

IF analysis of PECAM1/CD31 using anti-PECAM1/CD31 antibody (PA1950-1).
PECAM1/CD31 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PECAM1/CD31 Antibody (PA1950-1) overnight at 4°C. Fluoro594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-CD31/PECAM1 Antibody Picoband®

PECAM1 antibody

17 Publications

Reactive Species	Host	Application
Human	Rabbit	IF, IHC, WB

Tested Applications

PECAM1 antibody was positively detected by WB in:

human HUVEC whole cell, human THP-1 whole cell, human Jurkat whole cell, human U937 whole cell, human THP-1 whole cell, human HEL whole cell, human HepG2 whole cell, human PC-3 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

PECAM1 antibody was positively detected by IHC in:

human liver cancer tissue, human placenta tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

PECAM1 antibody was positively detected by IF in:

human liver cancer tissue, human placenta tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Product Information

Product Name

Anti-CD31/PECAM1 Antibody Picoband®
View all PECAM1 Antibodies

Form

Lyophilized

Uniprot

P16284

Gene ID

5175

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CD31.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

120-130 kDa

Calculated Molecular Weight

82.5 kDa

Background of PECAM1

PECAM-1 (Platelet endothelial cell adhesion molecule), also known as cluster of differentiation 31 (CD31) is a protein that in human is encoded by the PECAM1 gene found on chromosome 17. PECAM1 is a member of the immunoglobulin (Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and particular T-cell subsets. Using a PCR-based analysis of somatic cell hybrids, Gumina et al. (1996) mapped PECAM1 to chromosome 17 in the region 17q23-qter. Several adhesion molecules expressed on platelets and endothelium also localized to 17q. Xie and Muller (1996) mapped the Pecam1 gene to mouse chromosome 6, region F3-G1, by fluorescence in situ hybridization. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions, and PECAM-1 plays a key role in removing aged neutrophils from the body.

Cite This Product

Anti-CD31/PECAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1950-1)

Protocols

Western Blot protocol for PECAM1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for PECAM1 antibody

Review Protocol

Immunofluorescence (IF) protocol for PECAM1 antibody

Review Protocol

Publications

ZRANB1-mediated EZH2 stabilization drives S100B/S100A4 transcriptional activation and corneal neovascularization in alkali burns

Published: 2026-07-22

Journal: Biology Direct

Application: IF

Impact Factor: 5.50

Species: Mouse

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Anti-CD31/PECAM1 Antibody Picoband®

PECAM1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

ZRANB1-mediated EZH2 stabilization drives S100B/S100A4 transcriptional activation and corneal neovascularization in alkali burns

An intelligent bacterial capture nanodrug enhances immunotherapy for implant-associated infections by inducing cuproptosis-like death in bacteria

Muscone attenuates myocardial infarction by regulating ferroptosis of cardiomyocytes through Nrf2/system Xc-/GPX4 signaling pathway

FGF mimetic peptide-modified electrospun nanocomposite fibrous membranes for accelerating infectious diabetic wound healing by synergistic antibacterial and pro-angiogenesis effects

Efficacy assessment of glycyrrhetinic acid-modified liposomes loaded with doxorubicin hydrochloride and cucurbitine B for synergistic treatment of hepatocellular carcinoma

A self-elastic chitosan sponge reinforced with lauric acid-modified quaternized chitosan and attapulgite to treat noncompressible hemorrhage and facilitate wound healing

Vascularized characteristics and functional regeneration of three-dimensional cell reconstruction of oral mucosa equivalents based on vascular homeostasis phenotypic modification

Injectable and self-healable hydrogel based on pullulan polysaccharide loading platelet-rich plasma and metal-phenol network nanoparticles for infectious wound healing

Protective effect of Clinopodium chinense (Benth.) O. Kuntze against abnormal uterine bleeding in female rats

Co-expression of vasoactive intestinal peptide and protein gene product 9.5 surrounding the lumen of human Schlemm's canal

MicroRNA-34a induces transdifferentiation of glioma stem cells into vascular endothelial cells by targeting Notch pathway

Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling

EPHB4 Regulates Human Trophoblast Cell Line HTR-8/SVneo Function: Implications for the Role of EPHB4 in Preeclampsia

Astragalus polysaccharide protects human cardiac microvascular endothelial cells from hypoxia/reoxygenation injury: The role of PI3K/AKT, Bax/Bcl-2 and caspase-3

Dual blockade of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) exhibits potent anti-angiogenic effects

Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing

The up-regulation of endothelin-1 and down-regulation of miRNA-125a-5p, -155, and -199a/b-3p in human atherosclerotic coronary artery

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