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Pack Size:100μg/vial
Validated Species:Human
Application:IHC, WB
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Product Name Anti-PECAM-1/CD31 Antibody
SKU/Catalog Number PA1950-1
Description Rabbit IgG polyclonal antibody for Platelet endothelial cell adhesion molecule(PECAM1) detection. Tested with WB, IHC-P, IHC-F in Human.
Cite This Product Anti-PECAM-1/CD31 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1950-1)
Replacement Item This antibody may replace the following items: sc-133091|sc-13537|sc-1505|sc-1505-R|sc-1506|sc-1506-R|sc-20071|sc-28188|sc-31045|sc-365804|sc-376764|sc-53411|sc-59169|sc-59938|sc-65260|sc-71872|sc-81158|sc-8306 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human CD31(717-732aa RKAVPDAVESRYSRTE).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name PECAM1
Protein Name Platelet endothelial cell adhesion molecule
Molecular Weight 82536 MW
Protein Function Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). .
Tissue Specificity Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level). .
Sequence Similarities Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Subcellular Localization Isoform Long: Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
Uniprot ID P16284
Alternative Names Platelet endothelial cell adhesion molecule;PECAM-1;EndoCAM;GPIIA';PECA1;CD31;PECAM1;
Research Areas |signal transduction|cytoskeleton / ecm|cell adhesion|cell adhesion molecules|endothelial| cardiovascular|angiogenesis|angiogenic factors| immunology|cell type markers| kits/ lysates/ other|elisa kits|cd markers elisa kits|atherosclerotic proteins elisa kits|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Platelet endothelial cell adhesion molecule

PECAM-1(Platelet endothelial cell adhesion molecule), also known as cluster of differentiation 31(CD31) is a protein that in human is encoded by the PECAM1 gene found on chromosome 17. PECAM1 is a member of the immunoglobulin(Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and particular T-cell subsets. Using a PCR-based analysis of somatic cell hybrids, Gumina et al.(1996) mapped PECAM1 to chromosome 17 in the region 17q23-qter. Several adhesion molecules expressed on platelets and endothelium also localized to 17q. Xie and Muller(1996) mapped the Pecam1 gene to mouse chromosome 6, region F3-G1, by fluorescence in situ hybridization. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions, and PECAM-1 plays a key role in removing aged neutrophils from the body.

Anti-PECAM-1/CD31 Antibody Images

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Anti-PECAM-1/CD31 Antibody
Anti-CD31 antibody, PA1950-1, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-PECAM-1/CD31 Antibody
Anti-CD31 antibody, PA1950-1, IHC(P)
IHC(P): Human Placenta Tissue
Anti-PECAM-1/CD31 Antibody
Anti-CD31 antibody, PA1950-1, IHC(F)
IHC(F): Human Placenta Tissue
Anti-PECAM-1/CD31 Antibody
Anti-CD31 antibody, PA1950-1,Western blotting
All lanes: Anti CD31 (PA1950-1) at 0.5ug/ml
WB : Human Placenta Tissue Lysate at 50ug
Predicted bind size: 82 KD
Observed bind size: 82KD
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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd31 antibody, pecam 1 antibody, pecam antibody, pecam-1 antibody