Anti-Pyrophosphatase 1/PPA1 Antibody Picoband™

Inorganic Pyrophosphatase/PPA1 antibody

Boster Bio Anti-Pyrophosphatase 1/PPA1 Antibody Picoband™ catalog # A07485-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A07485-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Pyrophosphatase 1/PPA1 Antibody Picoband™

View all Inorganic Pyrophosphatase/PPA1 Antibodies

SKU/Catalog Number

A07485-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Pyrophosphatase 1/PPA1 Antibody Picoband™ catalog # A07485-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Pyrophosphatase 1/PPA1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07485-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Pyrophosphatase 1/PPA1 recombinant protein (Position: E7-N236).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A07485-1 is reactive to PPA1 in Human, Mouse, Rat

Applications

A07485-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of Inorganic Pyrophosphatase/PPA1

PPA1 is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. By radiation hybrid analysis, the PPA1 gene is mapped to chromosome 10q21.3.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For PPA1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q15181

Gene ID

5464

Gene Name

PPA1

Full Name

Inorganic pyrophosphatase

Weight

32.66kDa

Superfamily

PPase family

Alternative Names

diphosphate phosphohydrolase; EC 3.6.1.1; inorganic diphosphatase; inorganic pyrophosphatase 1; inorganic pyrophosphatase; IOPPP; IOPPPMGC111556; IPP1; PP1; PPA1; PPase; PPcytosolic inorganic pyrophosphatase; pyrophosphatase (inorganic) 1; pyrophosphatase (inorganic); pyrophosphatase 1; Pyrophosphate phospho-hydrolase; SID6-8061 PPA1 HEL-S-66p, IOPPP, PP, PP1, SID6-8061 inorganic pyrophosphatase 1 inorganic pyrophosphatase|PPase|cytosolic inorganic pyrophosphatase|diphosphate phosphohydrolase|epididymis secretory sperm binding protein Li 66p|inorganic diphosphatase 1|pyrophosphatase (inorganic) 1|pyrophosphatase 1|pyrophosphate phospho-hydrolase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PPA1, check out the PPA1 Infographic

PPA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PPA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-Pyrophosphatase 1/PPA1 Antibody Picoband™

Question

Do you have a BSA free version of anti-Pyrophosphatase 1/PPA1 antibody A07485-1 available?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Pyrophosphatase 1/PPA1 antibody A07485-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-19

Question

We are currently using anti-Pyrophosphatase 1/PPA1 antibody A07485-1 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-01

Answer

The anti-Pyrophosphatase 1/PPA1 antibody (A07485-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-01

Question

My question regarding product A07485-1, anti-Pyrophosphatase 1/PPA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07485-1 anti-Pyrophosphatase 1/PPA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-23

Question

Does anti-Pyrophosphatase 1/PPA1 antibody A07485-1 work for WB with adrenal gland?

R. Walker

Verified customer

Asked: 2017-07-13

Answer

According to the expression profile of adrenal gland, PPA1 is highly expressed in adrenal gland. So, it is likely that anti-Pyrophosphatase 1/PPA1 antibody A07485-1 will work for WB with adrenal gland.

Boster Scientific Support

Answered: 2017-07-13

Question

See attached the WB image, lot number and protocol we used for adrenal gland using anti-Pyrophosphatase 1/PPA1 antibody A07485-1. Please let me know if you require anything else.

M. Jackson

Verified customer

Asked: 2017-02-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-02-22

Size

Conjugation

how to order through PO

Total: $315

Size:100μg

SKU:A07485-1

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A07485-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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