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- Table of Contents
Cd4 antibody (A00344-2) performs well in ELISA, IHC, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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IHC analysis of CD4 using anti-CD4 antibody (A00344-2).
CD4 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD4 Antibody (A00344-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
CD4 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD4 Antibody (A00344-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-CD4 Antibody Picoband®
Cd4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | ELISA, IHC, WB |
Tested Applications
Cd4 antibody was positively detected by WB in:
mouse thymus tissue, mouse spleen tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Cd4 antibody was positively detected by IHC in:
mouse spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Mouse
Tested
Tested
Recommend
Recommend
Rat
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD4 Antibody Picoband®
View all CD4 Antibodies
View all CD4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat CD4 recombinant protein (Position: K28-I457).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Calculated Molecular Weight
51.4 kDa
Background of Cd4
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Cite This Product
Anti-CD4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00344-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
75%
Moderate Homology
Human
54%
Low homology
Rabbit
54%
Low homology
Monkey
53%
Low homology
Sheep
50%
Low homology
Dog
47%
Low homology
Cow
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Cd4 antibody
Immunohistochemistry (IHC) protocol for Cd4 antibody
Publications
Application:
Species:
Carrier-Free Nanoparticles Enhance Photothermal–Immune Therapy via Metabolic Reprogramming in Triple-Negative Breast Cancer
Published: 2026-07-11
Journal: Advanced Healthcare Materials
Application: FC
Impact Factor: 11.00
Species: Mouse
PMID: 42434841
Identification of Lymphangiogenesis-Related Diagnostic Model for Predicting Abdominal Aortic Aneurysm Onset and Progression and Validation of Lymphopoiesis in Abdominal Aortic Aneurysm
Published: 2025-11-01
Journal: MOLECULAR AND CELLULAR PROBES
Application: IHC
Impact Factor: 3.00
Species: Mouse
PMID: 41183720
Ultrasmall PtIr Bimetallic Nanozyme Treats Myocardial Infarction via Ischemic/Inflammatory Cardiac Microenvironment Remodeling
Published: 2025-04-02
Journal: ACS Nano
Application: IHC
Impact Factor: 16.00
Species: Rat
PMID: 40175295
The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis
Published: 2023-03-07
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IHC
Impact Factor: 8.20
Species: Mouse
PMID: 36889619
Controlled intracellular aggregation of magnetic particles improves permeation and retention for magnetic hyperthermia promotion and immune activation
Published: 2023-02-27
Journal: Theranostics
Application: WB
Impact Factor: 12.40
Species: Mouse
PMID: 36923543
Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation
Published: 2021-11-23
Journal: CELL TRANSPLANTATION
Application: IHC
Impact Factor: 4.06
Species: Rat,Mouse
PMID: 34814739
A NO/ROS/RNS cascaded-releasing nano-platform for gas/PDT/PTT/immunotherapy of tumors
Published: 2021-06-29
Journal: Biomaterials Science
Application: IHC
Impact Factor: 6.84
Species: Mouse
PMID: 34269777
Berberine Prolongs Mouse Heart Allograft Survival by Activating T Cell Apoptosis via the Mitochondrial Pathway
Published: 2021-02-25
Journal: Frontiers in Immunology
Application: IF,IHC
Impact Factor: 7.56
Species: Mouse
PMID: 33732240
Increased Macrophage Activation Inhibited by Tacrolimus in the Kidney of Diabetic Rats
Published: 2014-11-05
Journal: NEPHRON EXPERIMENTAL NEPHROLOGY
Application: IHC
Impact Factor: 1.65
Species: Rat
PMID: 25376933
High dose combination pertussis toxin induces autoimmune inner ear disease in Sprague-Dawley rats
Published: 2011-06-15
Journal: ACTA OTO-LARYNGOLOGICA
Application: IHC
Impact Factor: 1.20
Species: Rat
PMID: 21366505
Induction of immune tolerance with heart-thymus composite allotransplantation in rats
Published: 2005-10-01
Journal: JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLOGY
Impact Factor: 0.19
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Product Promise
We promise Anti-CD4 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00344-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026