Anti-MyD88 Antibody Picoband™

COX-2 antibody

Boster Bio Anti-MyD88 Antibody Picoband™ catalog # PB9148. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PB9148
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB

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Product Name

Anti-MyD88 Antibody Picoband™

View all COX-2 Antibodies

SKU/Catalog Number

PB9148

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MyD88 Antibody Picoband™ catalog # PB9148. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MyD88 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9148)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MyD88 recombinant protein (Position: A44-F264). Human MyD88 shares 84% and 83% amino acid (aa) sequences identity with mouse and rat MyD88, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9148 is reactive to PTGS2 in Human, Mouse, Rat

Applications

PB9148 is guaranteed for ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Background of COX-2

MYD88 (MYELOID DIFFERENTIATION PRIMARY RESPONSE GENE 88), is a protein that, in humans, is encoded by the MYD88 gene. MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL1Rs). And it is mapped on 3p22.2. MYD88 encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. Overexpression of MYD88 caused an increase in the level of transcription from the interleukin-8 promoter. The C-terminal domain of MYD88 has significant sequence similarity to the cytoplasmic domain of IL1RAP. Inhibiting the IL1R-MYD88 pathway in vivo could block the damage from acute inflammation that occurs in response to sterile cell death, and do so in a way that might not compromise tissue repair or host defense against pathogens.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For PTGS2 (Source: Uniprot.org, NCBI)

Uniprot ID

P35354

Gene ID

5743

Gene Name

PTGS2

Full Name

Prostaglandin G/H synthase 2

Weight

68.996kDa

Superfamily

prostaglandin G/H synthase family

Alternative Names

COX2; COX-2; COX2cyclooxygenase 2b; cyclooxygenase-2; EC 1.14.99; EC 1.14.99.1; GRIPGHS; hCox-2; PGG/HS; PGH synthase 2; PGHS-2; PHS II; PHS-2; PHS-II; prostaglandin G/H synthase 2; prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase); Prostaglandin-endoperoxide synthase 2; PTGS2 PTGS2 COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2 prostaglandin-endoperoxide synthase 2 prostaglandin G/H synthase 2|PGH synthase 2|PHS II|cyclooxygenase 2|cyclooxygenase 2b|prostaglandin H2 synthase 2|prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PTGS2, check out the PTGS2 Infographic

PTGS2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PTGS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PB9148 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response

Gastroprotective Effects of Periplaneta americana L. Extract Against Ethanol-Induced Gastric Ulcer in Mice by Suppressing Apoptosis-Related Pathways.

Combination of fasudil and celecoxib promotes the recovery of injured spinal cord in rats better than celecoxib or fasudil alone

Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response

Effect of cyclooxygenase-2 inhibition on the development of post-traumatic stress disorder in rats

MicroRNA-101 regulates the viability and invasion of cervical cancer cells

Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Downregulates the Expression of Protumor Factors Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in a GM-CSF Receptor-Independent Manner in Cervical Cancer Cells

Odontogenic epithelial proliferation is correlated with COX-2 expression in dentigerous cyst and ameloblastoma

Anti-inflammatory Effects of Phyllanthus emblica L on Benzopyrene-Induced Precancerous Lung Lesion by Regulating the IL-1?/miR-101/Lin28B Signaling Pathway

miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor

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5 Customer Q&As for Anti-MyD88 Antibody Picoband™

Question

We have seen staining in rat pancreas. Are there any suggestions? Is anti-MyD88 antibody supposed to stain pancreas positively?

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

From what I have seen in literature pancreas does express MYD88. From what I have seen in Uniprot.org, MYD88 is expressed in blood, dendritic cell, epidermal carcinoma, umbilical cord blood, pancreas, erythroleukemia, among other tissues. Regarding which tissues have MYD88 expression, here are a few articles citing expression in various tissues:
Dendritic cell, Pubmed ID: 8957090
Epidermal carcinoma, Pubmed ID: 9013863
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-05-04

Question

We need using your anti-MyD88 antibody for cellular response to lead ion studies. Has this antibody been tested with western blotting on cardiac muscle tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

We appreciate your inquiry. This PB9148 anti-MyD88 antibody is tested on rat lung tissue, cardiac muscle tissue, tissue lysate, hela whole cell lysate, mcf whole cell lysate, hepg2 whole cell lysate, jurkat whole cell lysate, raji whole cell lysate, intestinal cancer tissue, mouse spleen tissue, a549 cells. It is guaranteed to work for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-08

Question

My team were well pleased with the WB result of your anti-MyD88 antibody. However we have been able to see positive staining in erythroleukemia cytoplasm using this antibody. Is that expected? Could you tell me where is MYD88 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

Based on literature, erythroleukemia does express MYD88. Generally MYD88 expresses in cytoplasm. Regarding which tissues have MYD88 expression, here are a few articles citing expression in various tissues:
Dendritic cell, Pubmed ID: 8957090
Epidermal carcinoma, Pubmed ID: 9013863
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-08-26

Question

We are currently using anti-MyD88 antibody PB9148 for mouse tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

L. Taylor

Verified customer

Asked: 2016-03-04

Answer

The anti-MyD88 antibody (PB9148) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-03-04

Question

We have tried in the past anti-MyD88 antibody for WB on pancreas in a previous project. I am using rat, and We intend to use the antibody for ELISA next. We need examining pancreas as well as epidermal carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?

G. Kulkarni

Verified customer

Asked: 2015-06-30

Answer

I took a look at the website and datasheets of our anti-MyD88 antibody and it appears that PB9148 has been tested on rat in both WB and ELISA. Thus PB9148 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-06-30

Size

Conjugation

Total: $315

SKU:PB9148

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PB9148
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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