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Product Info Summary
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Anti-CD46 Antibody Picoband™
Boster Bio Anti-CD46 Antibody Picoband™ catalog # PB9848. Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD46 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9848)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CD46 (46-76aa ELPRPFEAMELKGTPKLFYAVGEKIEYKCKK), different from the related human sequence by twelve amino acids, and from the related rat sequence by nine amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
PB9848 is reactive to Cd46 in Mouse
PB9848 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD46 using anti-CD46 antibody (PB9848).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse testis tissue lysates,
Lane 2: NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD46 antigen affinity purified polyclonal antibody (Catalog # PB9848) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD46 at approximately 41 kDa, 70 kDa. The expected band size for CD46 is at 41 kDa.
Gene/Protein Information For Cd46 (Source: Uniprot.Org, NCBI)
Membrane cofactor protein
AHUS2; CD46 antigen; CD46 molecule, complement regulatory protein; CD46; MCP; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MIC10; TLXcomplement regulatory protein; trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen Cd46|Mc, Mcp|CD46 antigen, complement regulatory protein|membrane cofactor protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cd46, check out the Cd46 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd46: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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