Anti-CD9 Rabbit Monoclonal Antibody

CD9 antibody

Boster Bio Anti-CD9 Rabbit Monoclonal Antibody catalog # M01202. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M01202
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-CD9 Rabbit Monoclonal Antibody

View all CD9 Antibodies

SKU/Catalog Number

M01202

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD9 Rabbit Monoclonal Antibody catalog # M01202. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01202)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CED-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD9

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01202 is reactive to CD9 in Human, Mouse, Rat

Applications

M01202 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

17 kDa

Calculated molecular weight

25.416kDa

Background of CD9

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:100-1:500
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CD9 (Source: Uniprot.org, NCBI)

Gene Name

CD9

Full Name

CD9 antigen

Weight

25.416kDa

Superfamily

tetraspanin (TM4SF) family

Alternative Names

BA2; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 molecule; CD9; Cell growth-inhibiting gene 2 protein; DRAP-27; Leukocyte antigen MIC3; MIC3; motility related protein-1,5H9 antigen; Motility-related protein; MRP-1FLJ99568; p24; tetraspanin-29; TSPAN29; TSPAN-29; TSPAN29MIC3CD9 antigen (p24) CD9 BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29 CD9 molecule CD9 antigen|5H9 antigen|BA-2/p24 antigen|CD9 antigen (p24)|antigen CD9|cell growth-inhibiting gene 2 protein|leukocyte antigen MIC3|motility related protein-1|tetraspanin-29

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD9, check out the CD9 Infographic

CD9 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01202 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The bone marrow microenvironment at single-cell resolution
Species: Mouse

Wang D,Hao C,Zhang L,Zhang J,Liu S,Li Y,Qu Y,Zhao Y,Huang R,Wei J,Yao W.Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.Ecotoxicol Environ Saf.2020 Apr 1;192:110253.doi:10.1016/j.ecoenv.2020.110253.Epub
Species: Human,Mouse
M01202 usage in article: APP:WB, SAMPLE:FIBROBLAST, DILUTION:NA

Have you used Anti-CD9 Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-CD9 Rabbit Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

5 Customer Q&As for Anti-CD9 Rabbit Monoclonal Antibody

Question

We are interested in using your anti-CD9 Rabbit Monoclonal antibody for negative regulation of platelet aggregation studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

Thank you for your inquiry. This M01202 anti-CD9 Rabbit Monoclonal antibody is tested on hela cells. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-31

Question

We ordered your anti-CD9 Rabbit Monoclonal antibody for Flow Cytometry on platelet in a previous project. I am using rat, and We want to use the antibody for WB next. Our lab want to know about examining platelet as well as trigeminal ganglion in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

I looked at the website and datasheets of our anti-CD9 Rabbit Monoclonal antibody and it seems that M01202 has been tested on rat in both Flow Cytometry and WB. Thus M01202 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-06-27

Question

Our lab were satisfied with the WB result of your anti-CD9 Rabbit Monoclonal antibody. However we have seen positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD9 supposed to be expressed?

M. Jha

Verified customer

Asked: 2015-02-20

Answer

From literature, leukocyte does express CD9. Generally CD9 expresses in cell membrane. Regarding which tissues have CD9 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 8486348
Ovary, Pubmed ID: 15489334
Platelet, Pubmed ID: 2358073

Boster Scientific Support

Answered: 2015-02-20

Question

We are currently using anti-CD9 Rabbit Monoclonal antibody M01202 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

K. Yang

Verified customer

Asked: 2014-09-29

Answer

The anti-CD9 Rabbit Monoclonal antibody (M01202) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-09-29

Question

We have seen staining in human leukocyte. What should we do? Is anti-CD9 Rabbit Monoclonal antibody supposed to stain leukocyte positively?

A. Jackson

Verified customer

Asked: 2013-04-15

Answer

Based on literature leukocyte does express CD9. Based on Uniprot.org, CD9 is expressed in trigeminal ganglion, leukocyte, ovary, platelet, among other tissues. Regarding which tissues have CD9 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 8486348
Ovary, Pubmed ID: 15489334
Platelet, Pubmed ID: 2358073

Boster Scientific Support

Answered: 2013-04-15

Order DetailsPrice
M01202

100μL

$370
M01202-30ul

30μL sample

$99
M01202-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M01202
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.