Click image to see more details
Product Info Summary
| SKU: | A02982 |
|---|---|
| Size: | 500ug |
| Reactive Species: | Human, Chimpanzee |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD97 antigen CD97 Antibody
SKU/Catalog Number
A02982
Size
500ug
Form
Lyophilized
Description
Boster Bio Anti-CD97 antigen CD97 Antibody (Catalog # A02982). Tested in IF, WB applications. This antibody reacts with Human, Chimpanzee.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)
Cite This Product
Anti-CD97 antigen CD97 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02982)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.
Cross-reactivity
beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
Reactive Species
A02982 is reactive to CD97 in Human, Chimpanzee
Observed Molecular Weight
68 kDa
Calculated molecular weight
90.4 kDa
Background of CD97
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including colon, thyroid, stomach, and esophagus. One high affinity ligand that has been identified for CD97 is the integrin alpha 5 beta 1. CD97 engagement of endothelial alpha5 beta 1 results in increased angiogenesis. Signals generated through CD97 are thought to result in Rho activation and the regulation of motility. Anti-CD97 is ideal for researchers interested in cancer, angiogenesis, and inflammation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02982 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IF Microscopy: 1:1,000 - 1:5,000
WB: 1:1,000 - 1:5,000
This Protein A purified antibody has been tested for use in immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to isoform 1 of CD97 protein by western blotting in the appropriate cell lysate or extract.
Validation Images & Assay Conditions
Click image to see more details
Western blot using Boster's Protein A purified anti-CD97 antibody Lane 1: lysate from cells transfected with control DNA. Lane 2: bone marrow lysates taken from CD97 knockout mice. Lane 3: lysate from COS cells expressing Fc-CD97- (5EGF). Load: 10µL per lane. Primary Antibody: 1:1,000 dilution of the primary antibody was used. Secondary Antibody: Exposure: 10-sec. Results: The formation of the CD97 complex is currently under investigation. A shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. No staining was noted from bone marrow lysates taken from CD97 knockout mice. ~65 kDa appearing in all lanes is not known. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
Click image to see more details
Immunofluorescence Microscopy using Boster's Protein A purified anti-CD97 antibody shows staining of Fc-CD97-(5EGF) (panel D) in transfected COS cells. Panel A and C shows similar staining using pre-immune serum. Panel A and B show staining of mock transfected COS cells (no vector). A 1:2,500 dilution of the primary antibody was used. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
Specific Publications For Anti-CD97 antigen CD97 Antibody (A02982)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD97 antigen CD97 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CD97 antigen CD97 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
