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Product Info Summary
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Anti-CDK8 Antibody Picoband™
Boster Bio Anti-CDK8 Antibody Picoband™ catalog # A01493-1. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01493-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human Cdk8 recombinant protein (K355-Y464).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A01493-1 is reactive to CDK8 in Human
A01493-1 is guaranteed for ELISA, WB Boster Guarantee
Background of CDK8
Cell division protein kinase 8 is an enzyme that in humans is encoded by the CDK8 gene. It is mapped to 13q12.13. This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDK8 using anti-CDK8 antibody (A01493-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates,
Lane 6: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK8 antigen affinity purified polyclonal antibody (Catalog # A01493-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK8 at approximately 53 kDa. The expected band size for CDK8 is at 53 kDa.
Gene/Protein Information For CDK8 (Source: Uniprot.Org, NCBI)
Cyclin-dependent kinase 8
protein kinase superfamily
CDK8 protein kinase; CDK8; Cell division protein kinase 8; cyclin-dependent kinase 8; EC 2.7.11; EC 220.127.116.11; EC 18.104.22.168; K35; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; MGC126074; MGC126075; Protein kinase K35 CDK8 IDDHBA, K35 cyclin dependent kinase 8 cyclin-dependent kinase 8|CDK8 protein kinase|cell division protein kinase 8|mediator complex subunit CDK8|mediator of RNA polymerase II transcription subunit CDK8|protein kinase K35*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CDK8, check out the CDK8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CDK8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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