Anti-CDC25B Antibody Picoband™

CDC25B antibody

Boster Bio Anti-CDC25B Antibody Picoband™ catalog # A01899-1. Tested in Flow Cytometry, ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01899-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, WB

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Product Name

Anti-CDC25B Antibody Picoband™

View all CDC25B Antibodies

SKU/Catalog Number

A01899-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDC25B Antibody Picoband™ catalog # A01899-1. Tested in Flow Cytometry, ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CDC25B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01899-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human CDC25B recombinant protein (Position: M1-H486).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01899-1 is reactive to CDC25B in Human, Mouse, Rat

Applications

A01899-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Background of CDC25B

Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216; phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CDC25B (Source: Uniprot.Org, NCBI)

Uniprot ID

P30305

Gene ID

994

Gene Name

CDC25B

Full Name

M-phase inducer phosphatase 2

Weight

64.987kDa

Superfamily

MPI phosphatase family

Alternative Names

CDC25B; CDC25HU2; cell division cycle 25 homolog B (S. cerevisiae); cell division cycle 25 homolog B (S. pombe); cell division cycle 25B; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2 CDC25B cell division cycle 25B M-phase inducer phosphatase 2|CDC25 homolog B|dual specificity phosphatase Cdc25B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDC25B, check out the CDC25B Infographic

CDC25B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDC25B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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Total: $315

Size:100 μg

SKU:A01899-1

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A01899-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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