Product Info Summary
| SKU: | EK0782 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Human IL-1RA/IL1RN ELISA Kit PicoKine®
View all IL-1ra/IL-1F3/IL1RN ELISA kits
SKU/Catalog Number
EK0782
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-1RA/IL1RN ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL1RN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-1RA/IL1RN ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0782)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: R26-E177
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0782 is reactive to IL1RN in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0782 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-1ra/IL-1F3/IL1RN
The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0782-CAP | Anti-Human IL1RN Pre-coated 96-well strip microplate | 1 |
| EK0782-ST | Human IL1RN Standard | 2 vials, 10 ng/tube |
| EK0782-DA | Human IL1RN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.007 | 0.064 | 0.128 | 0.224 | 0.456 | 0.842 | 1.354 | 1.973 |
Data Example Images
Click image to see more details
Human IL-1RA PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-1ra/IL-1F3/IL1RN, Dilution ratio of 1:1, concentration in serum is around 800pg/ml.
Some articles we found to cite concentrations of IL-1ra/IL-1F3/IL1RN in samples: 20185814 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-1RA/IL1RN ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 57 | 296 | 803 | 56 | 318 | 731 |
| Standard deviation | 3.59 | 20.42 | 42.55 | 4.2 | 27.34 | 48.97 |
| CV (%) | 8.6% | 6.9% | 5.3% | 7.5% | 8.6% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-1ra/IL-1F3/IL1RN in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 57 | 52 | 59 | 58 | 56 | 2.69 | 4.8% |
| Sample 2 | 296 | 274 | 294 | 294 | 289 | 8.98 | 3.1% |
| Sample 3 | 803 | 845 | 834 | 932 | 853 | 47.86 | 5.6% |
Protein Target Info & Infographic
Gene/Protein Information For IL1RN (Source: Uniprot.Org, NCBI)
Uniprot ID
P18510
Gene ID
3557
Gene Name
IL1RN
Full Name
Interleukin-1 receptor antagonist protein
Weight
20.055kDa
Alternative Names
DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4 IL1RN DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4 interleukin 1 receptor antagonist interleukin-1 receptor antagonist protein|IL1 inhibitor|intracellular IL-1 receptor antagonist type II|intracellular interleukin-1 receptor antagonist (icIL-1ra)|type II interleukin-1 receptor antagonist
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL1RN, check out the IL1RN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL1RN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human IL-1RA/IL1RN ELISA Kit PicoKine® (EK0782)
EK0782 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Involvement of IL-6 and IL-1 receptor antagonist on intellectual disability
The effect of salusin-? on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs)
Esfahani, M., Saidijam, M., Goodarzi, M.T., Movahedian, A., & Najafi, R. (2017). Salusin-α Attenuates Inflammatory Responses in Vascular Endothelial Cells. Biochemistry (Moscow), 82(11), 1314–1323. doi: 10.1134/S0006297917110098
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Customer Reviews
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1 Reviews For Human IL-1RA/IL1RN ELISA Kit PicoKine®
0
Great kit--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews".)
Excellent
Source: Biocompare.com
| Applications | IL-1RA detection in rat serum |
|---|---|
| Sample | Rat serum |
| Detection | -- |
"I used this kit to detect a recombinant IL1RA in rat serum and it worked very well. The procedure was easy to follow and my standard curve was right on target. If needed we will order this kit and use it again. The kit was able to detect our rIL1RA but was less sensitive in detecting an altered formulation of the protein."
Unattributed Review*
Verified customer
Submitted 2016-08-24
Customer Q&As
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7 Customer Q&As for Human IL-1RA/IL1RN ELISA Kit PicoKine®
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-1ra using the EK0782 Human IL-1ra Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-06-14
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-06-14
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-05-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-05-27
Question
Q: What is the optimal O.D. value for IL-1ra ELISA kit? I performed your IL-1ra ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-1ra even though the O.D. values are not very high?
L. Nelson
Verified customer
Asked: 2019-05-20
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-1ra ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of IL-1ra that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2019-05-20
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of IL-1ra in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-1ra ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2016-07-18
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-07-18
Question
Q: how to analyze ELISA data? I have performed IL-1ra level in serum.
Verified Customer
Verified customer
Asked: 2016-07-08
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-07-08
Question
Q: can I use citrate plasma as samples in Human IL-1ra Picokine® ELISA Kit (Catalog # EK0782)?
P. Banerjee
Verified customer
Asked: 2015-09-14
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-1ra causing degradation of its protein structure. IL-1ra may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-1ra ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-09-14
