Overview
| Product Name |
Human IL-1RA/IL1RN ELISA Kit PicoKine™
See more IL-1ra/IL-1F3/IL1RN products |
| Catalog# |
EK0782 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Human IL-1RA/IL1RN ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL1RN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 2pg/ml. |
| Cite This Product |
Human IL-1RA/IL1RN ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0782)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<2 pg/ml |
| Assay Range |
31.2 pg/ml - 2,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: R26-E177 |
| Antibody Clonalities |
Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For IL1RN (Source: Uniprot.org, NCBI)
| Uniprot Id | P18510 |
|---|
| NCBI Gene Id | 3557 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | IL1RN |
|---|
| Protein Name | Interleukin-1 receptor antagonist protein |
|---|
| Alternative Names | IL-1ra/IL-1F3/IL1RN|DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4 |
|---|
| Molecular Weight | 20055 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IL1RN, check out the IL1RN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for IL1RN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the IL1RN infographic
Kit Components And Assay QC Data Details Of Human IL-1RA/IL1RN ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0782-CAP |
Anti-Human IL1RN Pre-coated 96-well strip microplate | 1 |
| EK0782-ST | Human IL1RN Standard | 2 vials, 10 ng/tube |
| EK0782-DA | Human IL1RN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0782 Human IL-1RA/IL1RN ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.007 | 0.128 | 0.224 | 0.456 | 0.842 | 1.354 | 1.973 | 2.272 |
We measured random samples of Human IL-1RA/IL1RN ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 57 | 296 | 803 | 56 | 318 | 731 |
| Standard deviation | 3.59 | 20.42 | 42.55 | 4.2 | 27.34 | 48.97 |
| CV (%) | 8.6% | 6.9% | 5.3% | 7.5% | 8.6% | 6.7% |
We measured the performance consistency of Human IL-1RA/IL1RN ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 57 | 52 | 59 | 58 | 56 | 2.69 | 4.8% |
| Sample 2 | 296 | 274 | 294 | 294 | 289 | 8.98 | 3.1% |
| Sample 3 | 803 | 845 | 834 | 932 | 853 | 47.86 | 5.6% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 2
| The effect of salusin-? on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs) |
PubMed ID 29942332 |
| Esfahani, M., Saidijam, M., Goodarzi, M.T., Movahedian, A., & Najafi, R. (2017). Salusin-α Attenuates Inflammatory Responses in Vascular Endothelial Cells. Biochemistry (Moscow), 82(11), 1314–1323. doi: 10.1134/S0006297917110098 |
PubMed ID 2922315 |
1 reviews
"Great kit"
Excellent, submitted by Unattributed Review* on
2016-08-24
Source: Biocompare.com
"Great kit"
--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews".)
| Applications | IL-1RA detection in rat serum |
|---|
| Sample | Rat serum |
|---|
| Detection | -- |
|---|
"I used this kit to detect a recombinant IL1RA in rat serum and it worked very well. The procedure was easy to follow and my standard curve was right on target. If needed we will order this kit and use it again. The kit was able to detect our rIL1RA but was less sensitive in detecting an altered formulation of the protein."
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0782 Human IL-1RA/IL1RN ELISA Kit PicoKine™
7 Related Questions
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-1ra using the EK0782 Human IL-1ra Picokine® ELISA Kit?
Verified Customer
Asked: 2020-06-14
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-06-14
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2020-05-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-05-27
Question
Q: What is the optimal O.D. value for IL-1ra ELISA kit? I performed your IL-1ra ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-1ra even though the O.D. values are not very high?
L. Nelson
Asked: 2019-05-20
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-1ra ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of IL-1ra that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2019-05-20
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of IL-1ra in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Asked: 2018-05-29
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-1ra ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2016-07-18
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-07-18
Question
Q: how to analyze ELISA data? I have performed IL-1ra level in serum.
Verified Customer
Asked: 2016-07-08
Boster Scientific Support
Answered: 2016-07-08
Question
Q: can I use citrate plasma as samples in Human IL-1ra Picokine® ELISA Kit (Catalog # EK0782)?
P. Banerjee
Asked: 2015-09-14
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-1ra causing degradation of its protein structure. IL-1ra may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-1ra ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-09-14
