|Validated Species:||Bovine, Human, Mouse, Rat|
Data & Images
|Product Name||Anti-CDC42 Antibody|
|Description||Rabbit IgG polyclonal antibody for Cell division control protein 42 homolog(CDC42) detection. Tested with WB, IHC-P in Human;Bovin;Mouse;Rat.|
|Cite This Product||Anti-CDC42 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1366)|
|Replacement Item||This antibody may replace the following items: sc-87|sc-6793|sc-26305|sc-26306|sc-9279|sc-9280|sc-34314|sc-34315|sc-6083|sc-8401|sc-390210|sc-6792|sc-7172 from Santa Cruz Biotechnology.|
|Validated Species||Bovine, Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human CDC42(121-138aa DDPSTIEKLAKNKQKPIT), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cell division control protein 42 homolog(Cdc42 homolog)|
|Molecular Weight||21259 MW|
|Protein Function||Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. .|
|Sequence Similarities||Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.|
|Subcellular Localization||Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome.|
|Alternative Names||Cell division control protein 42 homolog;G25K GTP-binding protein;CDC42;|
|Research Areas|||cell biology|cell cycle|cell division|cytokinesis| signal transduction|signaling pathway|g protein signaling|small g proteins|protein trafficking|vesicle transport|regulation| epigenetics and nuclear signaling|kinases/phosphatases| cancer|ras family||
Background for Cell division control protein 42 homolog(Cdc42 homolog)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CDC42 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Bovine, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CDC42 Antibody Images
Click the images to enlarge.
Lane 1: Recombinant Human CDC42 Protein 10ng
Lane 2: Recombinant Human CDC42 Protein 5ng
Lane 3: Recombinant Human CDC42 Protein 2.5ng
IHC(P): Human Mammary Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,