Anti-CDC42 Antibody

Boster Bio Anti-CDC42 Antibody catalog # PA1366. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA1366
Size: 100μg/vial
Reactive Species: Bovine, Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CDC42 Antibody

See all CDC42 products

SKU/Catalog Number

PA1366

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDC42 Antibody catalog # PA1366. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDC42 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1366)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human CDC42 (121-138aa DDPSTIEKLAKNKQKPIT), identical to the related mouse and rat sequences.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1366 is reactive to CDC42 in Bovine, Human, Mouse, Rat

Applications

PA1366 is guaranteed for IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Bovine, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For CDC42 (Source: Uniprot.Org, NCBI)

Uniprot ID

P60953

Gene ID

998

Gene Name

CDC42

Full Name

Cell division control protein 42 homolog

Weight

21.259kDa

Superfamily

small GTPase superfamily

Alternative Names

CDC42; CDC42Hs; cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein, 25kDa); cell division cycle 42 (GTP-binding protein, 25kD); dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; G25K; growth-regulating protein; GTP-binding protein, 25kD; small GTP binding protein CDC42 CDC42 CDC42Hs, G25K, TKS cell division cycle 42 cell division control protein 42 homolog|G25K GTP-binding protein|GTP binding protein, 25kDa|dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))|dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))|growth-regulating protein|small GTP binding protein CDC42

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDC42, check out the CDC42 Infographic

CDC42 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDC42: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1366 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Han F1, Zhuang TT1, Chen JJ1, Zhu XL1,2, Cai YF1, Lu YP1. PLoS One. 2017 Sep 21;12(9):e0185102. doi: 10.1371/journal.pone.0185102. eCollection 2017. Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal ...

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17 Customer Q&As for Anti-CDC42 Antibody

Question

I was wanting to use your anti-CDC42 antibody for WB for human brain placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain placenta identification?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

You can see on the product datasheet, PA1366 anti-CDC42 antibody has been tested for IHC, WB on bovine, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-24

Question

Is a blocking peptide available for product anti-CDC42 antibody (PA1366)?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

We do provide the blocking peptide for product anti-CDC42 antibody (PA1366). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-28

Question

We have been able to see staining in mouse neutrophil. What should we do? Is anti-CDC42 antibody supposed to stain neutrophil positively?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

From what I have seen in literature neutrophil does express CDC42. From what I have seen in Uniprot.org, CDC42 is expressed in testis, fetal brain, placenta, brain placenta, cervix, placenta uterus, brain, cajal-retzius cell fetal brain cortex, neutrophil, embryo, among other tissues. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704

Boster Scientific Support

Answered: 2020-01-30

Question

Is this PA1366 anti-CDC42 antibody reactive to the isotypes of CDC42?

J. Zhang

Verified customer

Asked: 2019-11-05

Answer

The immunogen of PA1366 anti-CDC42 antibody is A synthetic peptide corresponding to a sequence in the middle region of human CDC42(121-138aa DDPSTIEKLAKNKQKPIT), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-05

Question

Our team were well pleased with the WB result of your anti-CDC42 antibody. However we have seen positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is CDC42 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

From literature, placenta does express CDC42. Generally CDC42 expresses in cell membrane. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704

Boster Scientific Support

Answered: 2019-10-08

Question

My question regarding product PA1366, anti-CDC42 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1366 anti-CDC42 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-05

Question

We ordered your anti-CDC42 antibody for WB on cajal-retzius cell fetal brain cortex in a previous project. I am using bovine, and We are going to use the antibody for IHC next. I am interested in examining cajal-retzius cell fetal brain cortex as well as brain placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

I looked at the website and datasheets of our anti-CDC42 antibody and it appears that PA1366 has been validated on bovine in both WB and IHC. Thus PA1366 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-05-27

Question

Is there a BSA free version of anti-CDC42 antibody PA1366 available?

Verified Customer

Verified customer

Asked: 2019-03-11

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDC42 antibody PA1366 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-03-11

Question

Will anti-CDC42 antibody PA1366 work on bovine WB with placenta?

L. Anderson

Verified customer

Asked: 2018-08-30

Answer

Our lab technicians have not validated anti-CDC42 antibody PA1366 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CDC42 antibody PA1366 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine placenta in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-30

Question

I see that the anti-CDC42 antibody PA1366 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-03-19

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-19

Question

Does PA1366 anti-CDC42 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-12-29

Answer

As indicated on the product datasheet, PA1366 anti-CDC42 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-12-29

Question

I am looking for using your anti-CDC42 antibody for cd28 dependent vav1 pathway studies. Has this antibody been tested with western blotting on mammary cancer tissue? We would like to see some validation images before ordering.

F. Anderson

Verified customer

Asked: 2016-05-03

Answer

I appreciate your inquiry. This PA1366 anti-CDC42 antibody is validated on mammary cancer tissue. It is guaranteed to work for IHC, WB in bovine, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-05-03

Question

My lab would like to test anti-CDC42 antibody PA1366 on human brain placenta for research purposes, then I may be interested in using anti-CDC42 antibody PA1366 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Mangal

Verified customer

Asked: 2015-05-26

Answer

The products we sell, including anti-CDC42 antibody PA1366, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-05-26

Question

Would anti-CDC42 antibody PA1366 work for WB with brain placenta?

L. Johnson

Verified customer

Asked: 2014-07-31

Answer

According to the expression profile of brain placenta, CDC42 is highly expressed in brain placenta. So, it is likely that anti-CDC42 antibody PA1366 will work for WB with brain placenta.

Boster Scientific Support

Answered: 2014-07-31

Question

Please see the WB image, lot number and protocol we used for brain placenta using anti-CDC42 antibody PA1366. Please let me know if you require anything else.

C. Anderson

Verified customer

Asked: 2014-06-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-06-16

Question

We are currently using anti-CDC42 antibody PA1366 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says bovine, human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

E. Kulkarni

Verified customer

Asked: 2013-11-07

Answer

The anti-CDC42 antibody (PA1366) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-11-07

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain placenta using anti-CDC42 antibody PA1366. Let me know if you need anything else.

S. Parker

Verified customer

Asked: 2013-07-30

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-07-30

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