Rabbit IgG polyclonal antibody for Cell division control protein 42 homolog(CDC42) detection. Tested with WB, IHC-P in Human;Bovin;Mouse;Rat. Cited in 1 publication(s).
Anti-CDC42 Antibody Info At A Glance
|Reactivity:||Bovine, Human, Mouse, Rat|
|Product Name||Anti-CDC42 Antibody
See all CDC42 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CDC42 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Bovine. CDC42 information: Molecular Weight: 21259 MW; Subcellular Localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome.|
|Cite This Product||Anti-CDC42 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1366)|
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|Anti-CDC42 Antibody may replace the following items: sc 87|sc 6793|sc 26305|sc 26306|sc 9279|sc 9280|sc 34314|sc 34315|sc 6083|sc 8401|sc 390210|sc 6792|sc 7172.|
|Reactivity/Species||Bovine, Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human CDC42(121-138aa DDPSTIEKLAKNKQKPIT), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CDC42 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||998|
|Species Of This Entry||Human|
|Protein Name||Cell division control protein 42 homolog|
|Superfamily||small GTPase superfamily|
|Alternative Names||CDC42|CDC42; CDC42Hs; cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein, 25kDa); cell division cycle 42 (GTP-binding protein, 25kD); dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; G25K; growth-regulating protein; GTP-binding protein, 25kD; small GTP binding protein CDC42|
|Post Tranlational Modifications||Phosphorylation; Ribosylation; Cleavage; Prenylation; Dephosphorylation; Ubiquitination; Geranylgeranylation; Deamidation; Methylation; Myristoylation; Palmitoylation; Farnesylation; Acetylation; Amidation; Oxidation; Reduction; Amination; Sumoylation; Phosphorothioation; Acylation|
|Subcellular Localization||Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Cell projection, dendrite. Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase (PubMed:15642749). Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells (PubMed:15642749). Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1 (PubMed:15642749). Colo|
|Gene Location||1p36.12, on Chromasome 1, gene sequence: NC_000001.11 (22052709..22101360)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CDC42 (Source: Uniprot.org, NCBI)
|Protein Function||Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation (By similarity). Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.|
|Interacting Proteins||ABCA1; APC; ARHGAP1; ARHGEF7; BAIAP2; CDC42BPA; CDC42EP1; CDC42EP2; IQGAP1; ITSN1; LRRK2; MAP3K11; MAPK8; MARK4; Mcf2l; PAK1; PAK2; PAK3; Pak3; PAK4; PAK5; PARD6A; PARD6B; Pard6b; PARD6G; PREX1; PRKCI; TNK2; WAS; WASL; Wasl|
|Research Areas||Breast Cancer; Cancer; Phospho-Specific; Signal Transduction|
|Related Diseases||Tissue Adhesions; Malignant Neoplasms; Neoplasms; Cell Invasion; Neoplasm Metastasis; Cell Transformation, Neoplastic; Wiskott-aldrich Syndrome; Carcinoma; Neoplasm Invasiveness; Nervousness; Malignant Neoplasm Of Breast; Malignant Paraganglionic Neoplasm; Inflammation; Mammary Neoplasms; Tumor Angiogenesis; Metaplastic Cell Transformation; Protrusion; Neuroblastoma; Salmonella Infections; Tumor Progression|
|Related Pathways||Cell Migration; Localization; Cell Motility; Cell Cycle; Secretion; Cell Adhesion; Cell Proliferation; Cell Growth; Cell Division; Endocytosis; Cytokinesis; Transport; Phagocytosis; Cell-cell Adhesion; Chemotaxis; Exocytosis; Rna Interference; Angiogenesis; Cell Death; Virulence|
|Background||Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene. CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein(N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.|
|Scientific References||PMID: 2122236 by Munemitsu S., et al. Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42.|
PMID: 2124704 by Shinjo K., et al. Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42.
Our Boster Quality Guarantee for Anti-CDC42 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Bovine, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Recombinant Human CDC42 Protein 10ng
Lane 2: Recombinant Human CDC42 Protein 5ng
Lane 3: Recombinant Human CDC42 Protein 2.5ng
IHC(P): Human Mammary Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 1
|Han F1, Zhuang TT1, Chen JJ1, Zhu XL1,2, Cai YF1, Lu YP1. PLoS One. 2017 Sep 21;12(9):e0185102. doi: 10.1371/journal.pone.0185102. eCollection 2017. Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal ...||PubMed ID 28934273|
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Note: sample size takes longer to process, average 5-7 days.
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