Anti-CDC42 Antibody Picoband™

Boster Bio Anti-CDC42 Antibody Picoband™ catalog # A00119. Tested in ELISA, Flow Cytometry, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00119
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC-P, WB

Product Name

Anti-CDC42 Antibody Picoband™

See all CDC42 products

SKU/Catalog Number

A00119

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDC42 Antibody Picoband™ catalog # A00119. Tested in ELISA, Flow Cytometry, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDC42 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00119)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human CDC42 recombinant protein (Position: K16-C188).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00119 is reactive to CDC42 in Human, Mouse, Rat

Applications

A00119 is guaranteed for ELISA, Flow Cytometry, IHC-P, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CDC42 (Source: Uniprot.Org, NCBI)

Uniprot ID

P60953

Gene ID

998

Gene Name

CDC42

Full Name

Cell division control protein 42 homolog

Weight

21.259kDa

Superfamily

small GTPase superfamily

Alternative Names

CDC42; CDC42Hs; cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein, 25kDa); cell division cycle 42 (GTP-binding protein, 25kD); dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; G25K; growth-regulating protein; GTP-binding protein, 25kD; small GTP binding protein CDC42 CDC42 CDC42Hs, G25K, TKS cell division cycle 42 cell division control protein 42 homolog|G25K GTP-binding protein|GTP binding protein, 25kDa|dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))|dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))|growth-regulating protein|small GTP binding protein CDC42

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDC42, check out the CDC42 Infographic

CDC42 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDC42: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-CDC42 Antibody Picoband™

Question

My question regards using your anti-CDC42 antibody for negative regulation of protein complex assembly studies. Has this antibody been tested with western blotting on rat thymus tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

Thanks for your inquiry. This A00119 anti-CDC42 antibody is validated on human hela, k562 whole cell lysates, hela whole cell lysates, rat thymus tissue, lung tissue, mouse sp20 whole cell lysates. It is guaranteed to work for ELISA, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-10

Question

We bought anti-CDC42 antibody for WB on cervix a few years ago. I am using human, and I plan to use the antibody for IHC-P next. I am interested in examining cervix as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

I have checked the website and datasheets of our anti-CDC42 antibody and it seems that A00119 has been tested on human in both WB and IHC-P. Thus A00119 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-06-10

Question

We are currently using anti-CDC42 antibody A00119 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

F. Krishna

Verified customer

Asked: 2017-08-30

Answer

The anti-CDC42 antibody (A00119) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-08-30

Question

My boss were satisfied with the WB result of your anti-CDC42 antibody. However we have been able to see positive staining in embryo cell membrane using this antibody. Is that expected? Could you tell me where is CDC42 supposed to be expressed?

G. Jha

Verified customer

Asked: 2017-08-22

Answer

From literature, embryo does express CDC42. Generally CDC42 expresses in cell membrane. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704

Boster Scientific Support

Answered: 2017-08-22

Question

We have been able to see staining in mouse placenta. What should we do? Is anti-CDC42 antibody supposed to stain placenta positively?

Verified Customer

Verified customer

Asked: 2017-08-15

Answer

From what I have seen in literature placenta does express CDC42. From what I have seen in Uniprot.org, CDC42 is expressed in testis, fetal brain, placenta, brain placenta, cervix, placenta uterus, brain, cajal-retzius cell fetal brain cortex, neutrophil, embryo, among other tissues. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704

Boster Scientific Support

Answered: 2017-08-15

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A00119
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