Anti-Protein C/PROC Antibody
Rabbit IgG polyclonal antibody for Vitamin K-dependent protein C(PROC) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human. Cited in 10 publication(s).
Anti-Protein C/PROC Antibody Info At A Glance
|Applications:||Flow Cytometry, IHC, ICC, WB|
|Product Name||Anti-Protein C/PROC Antibody
See all Coagulation Factor XIV/Protein C primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Protein C/PROC detection. Host: Rabbit.Size: 100μg/vial. Tested applications: Flow Cytometry. Reactive species: Human. Protein C/PROC information: Molecular Weight: 52071 MW; Subcellular Localization: Secreted ; Tissue Specificity: Plasma; synthesized in the liver.|
|Cite This Product||Anti-Protein C/PROC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1682)|
|Similar Products From
|Anti-Protein C/PROC Antibody may replace the following items: sc 271493|sc 271862|sc 292686|sc 292687|sc 365491|sc 365672|sc 365673|sc 373937|sc 377175|sc 46228|sc 46229|sc 46230|sc 46231|sc 52718|sc 52719|sc 59652|sc 65958|sc 65959|sc 67021|sc 69662|sc 80504|sc 81682|sc 81683|sc 82714.|
|Applications||Flow Cytometry, IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Protein C(446-461aa HGHIRDKEAPQKSWAP).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For PROC (Source: Uniprot.org, NCBI)
|NCBI Gene Id||5624|
|Species Of This Entry||Human|
|Protein Name||Vitamin K-dependent protein C|
|Superfamily||peptidase S1 family|
|Alternative Names||Coagulation Factor XIV/Protein C|Anticoagulant protein C; APC; Autoprothrombin IIA; Blood coagulation factor XIV; Coagulation Factor XIV; EC 3.4.21; EC 126.96.36.199; PC; PROC; PROC1; protein C (inactivator of coagulation factors Va and VIIIa); Protein C; vitamin K-dependent protein C|
|Post Tranlational Modifications||Phosphorylation; Nitration; Oxidation; Reduction; Carbamylation; Glycosylation; Dephosphorylation|
|Subcellular Localization||Secreted. Golgi apparatus. Endoplasmic reticulum.|
|Gene Location||2q14.3, on Chromasome 2, gene sequence: NC_000002.12 (127418143..127429246)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For PROC (Source: Uniprot.org, NCBI)
|Protein Function||Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).|
|Tissue Specificity||Plasma; synthesized in the liver.|
|Research Areas||Apoptosis; Cancer|
|Related Diseases||Salmonella Infections; Tuberculosis; Liver Diseases; Infective Disorder; Pain; Malignant Neoplasms; Neoplasms; Fracture; Congenital Heart Defects; Syphilis; Liver Dysfunction; Atherosclerosis; Staphylococcal Infections; Abscess; Chromosome Inversion; Cancer Patients And Suicide And Depression; Heart Diseases; Depressive Disorder; Stenosis; Cerebrovascular Accident|
|Related Pathways||Transport; Translation; Virulence; Mating; Secretion; Transposition; Urea Cycle; Fermentation; Coagulation; Pathogenesis; Cell Activation; Fertilization; Aging; Hypersensitivity; Sporulation; Reflex; Oligopeptide Transport; Antiport; Nitrogen Fixation; Cell Cycle|
|Background||Protein C(PROC), also called PC, is a zymogenic(inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped on 2q14.3. The PROC gene contains 8 exons and spans about 11 kb by Foster et al. The conversion of protein C to a protease with anticoagulant function by thrombin requires as a cofactor thrombomodulin, an endothelial cell membrane protein. Riewald et al. demonstrated that activated protein C uses the endothelial cell protein C receptor as a coreceptor for cleavage of protease-activated receptor-1 on endothelial cells. Faust et al. demonstrated that the endothelial pathways required for protein C activation are impaired in severe meningococcal sepsis. They stated that improvement in the outcome of children with meningococcal sepsis who were treated with unactivated protein C concentrates had been described in case reports and in 1 uncontrolled series.|
|Scientific References||PMID: 2991859 by Beckmann R.J., et al. The structure and evolution of a 461 amino acid human protein C precursor and its messenger RNA, based upon the DNA sequence of cloned human liver cDNAs.|
PMID: 2991887 by Foster D.C., et al. The nucleotide sequence of the gene for human protein C.
Our Boster Quality Guarantee for Anti-Protein C/PROC Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: JURKAT Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: HELA Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Protein C antigen affinity purified polyclonal antibody (Catalog # PA1682) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Protein C at approximately 36KD. The expected band size for Protein C is at 52KD.
Overlay histogram showing A549 cells stained with PA1682 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROC Antibody (PA1682,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein C was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Protein C Antibody (PA1682) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 10
|Aksu I, Baykara B, Kiray M, Gurpinar T, Sisman Ar, Ekerbicer N, Tas A, Gokdemir-Yazar O, Uysal N. Biotech Histochem. 2013 May;88(3-4):194-201. Doi: 10.3109/10520295.2012.758311. Epub 2013 Jan 21. Serum Igf-1 Levels Correlate Negatively To Liver Da...||PubMed ID 23331186|
|Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...||PubMed ID 26022197|
|Li Jt, Wang Lf, Zhao Yl, Yang T, Li W, Zhao J, Yu F, Wang L, Meng Yl, Liu Nn, Zhu Xs, Gao Cf, Jia Lt, Yang Ag. Breast Cancer Res. 2014 Oct 14;16(5):454. Doi: 10.1186/S13058-014-0454-2. Nuclear Factor Of Activated T Cells 5 Maintained By Hotair Sup...||PubMed ID 25311085|
|Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.||PubMed ID 21310960|
|Ban Jb, Fan Xw, Huang Q, Li Bf, Chen C, Zhang Hc, Xu Sq. Plos One. 2014 May 16;9(5):E97607. Doi: 10.1371/Journal.Pone.0097607. Ecollection 2014. Mono-(2-Ethylhexyl) Phthalate Induces Injury In Human Umbilical Vein Endothelial Cells.||PubMed ID 24836450|
|Bao Hj, Liu Y, Qin Jh, Xu Cs, Hei Nn, Jaber Jr, Chen Qs. Res Vet Sci. 2011 Dec;91(3):E16-24. Doi: 10.1016/J.Rvsc.2011.02.015. Epub 2011 Apr 12. An Immunohistochemical Study Of S-100 Protein In The Intestinal Tract Of Chinese Soft-Shelled Turtle, P...||PubMed ID 21486673|
|Tang Y, Li Y, Yu H, Gao C, Liu L, Chen S, Xing M, Liu L, Yao P. J Nutr Biochem. 2014 Jun;25(6):675-82. Doi: 10.1016/J.Jnutbio.2014.02.009. Epub 2014 Mar 19. Quercetin Prevents Ethanol-Induced Iron Overload By Regulating Hepcidin Through The Bmp6/S...||PubMed ID 24746831|
|Zhang S, Zhang M, Goldstein S, Li Y, Ge J, He B, Ruiz G. Cell Biochem Biophys. 2013 Mar;65(2):249-55. Doi: 10.1007/S12013-012-9428-0. The Effect Of C-Fos On Acute Myocardial Infarction And The Significance Of Metoprolol Intervention In A Rat Model.||PubMed ID 23054911|
|Zhang S, He B, Goldstein S, Ge J, Wang Z, Ruiz G. Virol J. 2010 Oct 27;7:285. Doi: 10.1186/1743-422X-7-285. The Expression And Significance Of Proto-Oncogene C-Fos In Viral Myocarditis.||PubMed ID 20979618|
|Chen Aq, Liu Zw, Yang Zg, Leng Xj. Comp Biochem Physiol B Biochem Mol Biol. 2012 Sep;163(1):121-8. Doi: 10.1016/J.Cbpb.2012.05.009. Epub 2012 May 18. Characterization Of Bmp15 And Its Regulation By Human Chorionic Gonadotropin In The Follicle Of G...||PubMed ID 22613815|
Order Product (PA1682)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.