Anti-CDC42 Rabbit Monoclonal Antibody

Boster Bio Anti-CDC42 Rabbit Monoclonal Antibody catalog # M00119. Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00119
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, WB

Product Name

Anti-CDC42 Rabbit Monoclonal Antibody

See all CDC42 products

SKU/Catalog Number

M00119

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CDC42 Rabbit Monoclonal Antibody catalog # M00119. Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDC42 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00119)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

IAE-3

Immunogen

A synthesized peptide derived from human CDC42

Reactive Species

M00119 is reactive to CDC42 in Human, Mouse, Rat

Applications

M00119 is guaranteed for IP, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
IP 1:30
Flow Cytometry 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CDC42 (Source: Uniprot.Org, NCBI)

Uniprot ID

P60953

Gene ID

998

Gene Name

CDC42

Full Name

Cell division control protein 42 homolog

Weight

21.259kDa

Superfamily

small GTPase superfamily

Alternative Names

CDC42; CDC42Hs; cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein, 25kDa); cell division cycle 42 (GTP-binding protein, 25kD); dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; G25K; growth-regulating protein; GTP-binding protein, 25kD; small GTP binding protein CDC42 CDC42 CDC42Hs, G25K, TKS cell division cycle 42 cell division control protein 42 homolog|G25K GTP-binding protein|GTP binding protein, 25kDa|dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))|dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))|growth-regulating protein|small GTP binding protein CDC42

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDC42, check out the CDC42 Infographic

CDC42 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDC42: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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16 Customer Q&As for Anti-CDC42 Rabbit Monoclonal Antibody

Question

Can you help my question with product M00119, anti-CDC42 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00119 anti-CDC42 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-17

Question

Is a blocking peptide available for product anti-CDC42 Rabbit Monoclonal antibody (M00119)?

B. Anderson

Verified customer

Asked: 2020-01-01

Answer

We do provide the blocking peptide for product anti-CDC42 Rabbit Monoclonal antibody (M00119). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-01

Question

I see that the anti-CDC42 Rabbit Monoclonal antibody M00119 works with IP, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-20

Question

you antibody using your anti-CDC42 Rabbit Monoclonal antibody for nuclear migration studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

Thank you for your inquiry. This M00119 anti-CDC42 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IP, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-01

Question

We are currently using anti-CDC42 Rabbit Monoclonal antibody M00119 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

The anti-CDC42 Rabbit Monoclonal antibody (M00119) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-02

Question

Please see the WB image, lot number and protocol we used for placenta using anti-CDC42 Rabbit Monoclonal antibody M00119. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-02

Question

Do you have a BSA free version of anti-CDC42 Rabbit Monoclonal antibody M00119 available?

A. Roberts

Verified customer

Asked: 2019-07-04

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDC42 Rabbit Monoclonal antibody M00119 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-04

Question

Our lab want to know about to test anti-CDC42 Rabbit Monoclonal antibody M00119 on mouse placenta for research purposes, then I may be interested in using anti-CDC42 Rabbit Monoclonal antibody M00119 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-01-29

Answer

The products we sell, including anti-CDC42 Rabbit Monoclonal antibody M00119, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-01-29

Question

We have tried in the past anti-CDC42 Rabbit Monoclonal antibody for IP on fetal brain a few months ago. I am using human, and I plan to use the antibody for WB next. I am looking for examining fetal brain as well as cervix in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-12-19

Answer

I looked at the website and datasheets of our anti-CDC42 Rabbit Monoclonal antibody and I see that M00119 has been tested on human in both IP and WB. Thus M00119 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-12-19

Question

Would M00119 anti-CDC42 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-11-07

Answer

You can see on the product datasheet, M00119 anti-CDC42 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-11-07

Question

I was wanting to use your anti-CDC42 Rabbit Monoclonal antibody for IP for mouse placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse placenta identification?

Verified Customer

Verified customer

Asked: 2018-09-25

Answer

You can see on the product datasheet, M00119 anti-CDC42 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-25

Question

Is this M00119 anti-CDC42 Rabbit Monoclonal antibody reactive to the isotypes of CDC42?

Verified Customer

Verified customer

Asked: 2018-06-21

Answer

The immunogen of M00119 anti-CDC42 Rabbit Monoclonal antibody is A synthesized peptide derived from human CDC42. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-06-21

Question

My colleagues were content with the WB result of your anti-CDC42 Rabbit Monoclonal antibody. However we have been able to see positive staining in neutrophil cell membrane using this antibody. Is that expected? Could you tell me where is CDC42 supposed to be expressed?

J. Carter

Verified customer

Asked: 2018-05-09

Answer

From literature, neutrophil does express CDC42. Generally CDC42 expresses in cell membrane. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704

Boster Scientific Support

Answered: 2018-05-09

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-CDC42 Rabbit Monoclonal antibody M00119. Let me know if you need anything else.

F. Roberts

Verified customer

Asked: 2017-11-20

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-20

Question

We have seen staining in human placenta uterus. Any tips? Is anti-CDC42 Rabbit Monoclonal antibody supposed to stain placenta uterus positively?

N. Jones

Verified customer

Asked: 2017-06-26

Answer

Based on literature placenta uterus does express CDC42. Based on Uniprot.org, CDC42 is expressed in testis, fetal brain, placenta, brain placenta, cervix, placenta uterus, brain, cajal-retzius cell fetal brain cortex, neutrophil, embryo, among other tissues. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704

Boster Scientific Support

Answered: 2017-06-26

Question

Does anti-CDC42 Rabbit Monoclonal antibody M00119 work for IP with placenta?

J. Jha

Verified customer

Asked: 2015-09-08

Answer

According to the expression profile of placenta, CDC42 is highly expressed in placenta. So, it is likely that anti-CDC42 Rabbit Monoclonal antibody M00119 will work for IP with placenta.

Boster Scientific Support

Answered: 2015-09-08

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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M00119
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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