Anti-CdcA3 Antibody

Cdca3 antibody

Boster Bio Anti-CdcA3 Antibody catalog # A10074. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A10074
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-CdcA3 Antibody

View all Cdca3 Antibodies

SKU/Catalog Number

A10074

Size

100μl

Form

Liquid

Description

Boster Bio Anti-CdcA3 Antibody catalog # A10074. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CdcA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10074)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2F1

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human CdcA3. at AA rangle: 190-270

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10074 is reactive to Cdca3 in Human, Mouse, Rat

Applications

A10074 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

28.708kDa

Background of Cdca3

Cybr (Pscdbp) is a new scaffold protein with significant homology to Tamalin. It contains a PDZ binding domain and a coiled-coil domain. Cybr is highly expressed in the immune system, and its expression seems to be common to nearly all immune cell populations (T cells, B cells, NK Macrophages, dendritic cells).  Other organs/tissues in which Cybr is expressed include lung, kidney, intestine and testes. Cybr may play an important in vivo role in cell migration, especially when modulated by pro-inflammatory cytokines. Anti-Cybr Antibody is suitable for researchers interested in the Research Areas of Chromatin, Immunology, Neuroscience, and Signal Transduction.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For Cdca3 (Source: Uniprot.org, NCBI)

Gene Name

Cdca3

Full Name

Cell division cycle-associated protein 3

Weight

28.708kDa

Alternative Names

C8; cell division cycle associated 3; FBXO52; Gene-rich cluster protein C8; GRCC8cell division cycle-associated protein 3; MGC2577; TOME1; TOME-1gene rich cluster, C8; trigger of mitotic entry 1; Trigger of mitotic entry protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cdca3, check out the Cdca3 Infographic

Cdca3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cdca3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-CdcA3 Antibody

Question

Do you have a BSA free version of anti-CdcA3 antibody A10074 available?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CdcA3 antibody A10074 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-19

Question

Here is the WB image, lot number and protocol we used for brain using anti-CdcA3 antibody A10074. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-22

Question

Will anti-CdcA3 antibody A10074 work for WB with brain?

Verified Customer

Verified customer

Asked: 2019-02-19

Answer

According to the expression profile of brain, CDCA3 is highly expressed in brain. So, it is likely that anti-CdcA3 antibody A10074 will work for WB with brain.

Boster Scientific Support

Answered: 2019-02-19

Question

I have a question about product A10074, anti-CdcA3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Miller

Verified customer

Asked: 2015-04-13

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10074 anti-CdcA3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-04-13

Question

Is a blocking peptide available for product anti-CdcA3 antibody (A10074)?

Z. Baker

Verified customer

Asked: 2013-10-15

Answer

We do provide the blocking peptide for product anti-CdcA3 antibody (A10074). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-10-15

Question

Would A10074 anti-CdcA3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

P. Li

Verified customer

Asked: 2013-07-01

Answer

As indicated on the product datasheet, A10074 anti-CdcA3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-07-01

Question

Our lab want to know about to test anti-CdcA3 antibody A10074 on rat brain for research purposes, then I may be interested in using anti-CdcA3 antibody A10074 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Jones

Verified customer

Asked: 2013-02-27

Answer

The products we sell, including anti-CdcA3 antibody A10074, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-02-27

Order DetailsPrice
A10074

100uL

$399

Free Secondary Antibody

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A10074
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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