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Product Info Summary
| SKU: | A10074 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CdcA3 Antibody
SKU/Catalog Number
A10074
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CdcA3 Antibody catalog # A10074. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CdcA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10074)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CDCA3. AA range:219-268
Reactive Species
A10074 is reactive to CDCA3 in Human, Mouse, Rat
Calculated molecular weight
29.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10074 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using CdcA3 Polyclonal Antibody
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Western blot analysis of lysates from HepG2 cells, using CDCA3 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using CDCA3 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CdcA3 Antibody (A10074)
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Customer Q&As
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7 Customer Q&As for Anti-CdcA3 Antibody
Question
Do you have a BSA free version of anti-CdcA3 antibody A10074 available?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CdcA3 antibody A10074 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-19
Question
Here is the WB image, lot number and protocol we used for brain using anti-CdcA3 antibody A10074. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-22
Question
Will anti-CdcA3 antibody A10074 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
According to the expression profile of brain, CDCA3 is highly expressed in brain. So, it is likely that anti-CdcA3 antibody A10074 will work for WB with brain.
Boster Scientific Support
Answered: 2019-02-19
Question
I have a question about product A10074, anti-CdcA3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Miller
Verified customer
Asked: 2015-04-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10074 anti-CdcA3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-04-13
Question
Is a blocking peptide available for product anti-CdcA3 antibody (A10074)?
Z. Baker
Verified customer
Asked: 2013-10-15
Answer
We do provide the blocking peptide for product anti-CdcA3 antibody (A10074). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-10-15
Question
Would A10074 anti-CdcA3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Li
Verified customer
Asked: 2013-07-01
Answer
As indicated on the product datasheet, A10074 anti-CdcA3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-07-01
Question
Our lab want to know about to test anti-CdcA3 antibody A10074 on rat brain for research purposes, then I may be interested in using anti-CdcA3 antibody A10074 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Jones
Verified customer
Asked: 2013-02-27
Answer
The products we sell, including anti-CdcA3 antibody A10074, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-02-27
