Human Ficolin-1/FCN1/M Ficolin PicoKine™ Quick ELISA Kit

Human Ficolin-1/FCN1/M Ficolin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human FCN1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml.

Product Info Summary

SKU: FEK1257
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin)
Assay Time: 90 Minutes

Product Name

Human Ficolin-1/FCN1/M Ficolin PicoKine™ Quick ELISA Kit

See all Ficolin-1 products

SKU/Catalog Number

FEK1257

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Ficolin-1/FCN1/M Ficolin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human FCN1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Ficolin-1/FCN1/M Ficolin PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1257)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A30-A326

Sensitivity

<20 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1257 is reactive to FCN1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin)

Application Guarantee

FEK1257 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog numberDescription QuantityVolume
FEK1257-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK1257-STHuman Ficolin-1 Standard 2 50 ng/tube
FEK1257-CHuman FCN1 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0030.1770.3130.5580.9261.4911.9722.357

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)14508641198421321869419651
Standard deviation92.8475.261111.1593.79512.951061.15
CV (%)6.4%5.5%5.6%7.1%5.9%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Ficolin-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11450136516051564149697.246.5%
Sample 286417641771279527987263.553.3%
Sample 319842226472164220458211471141.955.4%
*number of samples for each test n=16.

Gene/Protein Information For FCN1 (Source: Uniprot.Org, NCBI)

Uniprot ID

O00602

Gene ID

2219

Gene Name

FCN1

Full Name

Ficolin-1

Weight

35.078kDa

Superfamily

ficolin lectin family

Alternative Names

Collagen/fibrinogen domain-containing protein 1; FCN1; Fcna; FCNM; FCNMficolin-A; ficolin (collagen/fibrinogen domain containing) 1; ficolin (collagen/fibrinogen domain-containing) 1; Ficolin1; Ficolin-1; Ficolin-A; ficolin-alpha; M-ficolin FCN1 FCNM ficolin 1 ficolin-1|M-ficolin|ficolin (collagen/fibrinogen domain-containing) 1|ficolin-A|ficolin-alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FCN1, check out the FCN1 Infographic

FCN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FCN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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