Anti-Cyclin-dependent kinase 2 Cdk2 Antibody
Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 2(CDK2) detection. Tested with WB in Human;Mouse;Rat. Cited in 13 publication(s).
Anti-Cyclin-dependent kinase 2 Cdk2 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Cyclin-dependent kinase 2 Cdk2 Antibody
See all CDK2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CDK2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. CDK2 information: Molecular Weight: 33930 MW; Subcellular Localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).|
|Cite This Product||Anti-Cyclin-dependent kinase 2 Cdk2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1547)|
|Similar Products From
|Anti-Cyclin-dependent kinase 2 Cdk2 Antibody may replace the following items: sc 163|sc 748|sc 6248|sc 53220|sc 136191|sc 70829|sc 163 G.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Cdk2(277-298aa AKAALAHPFFQDVTKPVPHLRL), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CDK2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||1017|
|Species Of This Entry||Human|
|Protein Name||Cyclin-dependent kinase 2|
|Superfamily||protein kinase superfamily|
|Alternative Names||CDK2|cdc2-related protein kinase; CDK2; cell devision kinase 2; Cell division protein kinase 2; cyclin-dependent kinase 2; EC 2.7.11; EC 126.96.36.199; p33 protein kinase; p33(CDK2)|
|Post Tranlational Modifications||Phosphorylation; Dephosphorylation; Cleavage; Ubiquitination; Acetylation; Methylation; Reduction; Sumoylation; Phosphorothioation; Myristoylation; Geranylgeranylation; Demethylation; Farnesylation; Carboxylation; Deacetylation; Nitrosylation; Hydroxylation; Nitration; Biotinylation|
|Subcellular Localization||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).|
|Gene Location||12q13.2, on Chromasome 12, gene sequence: NC_000012.12 (55966830..55972789)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CDK2 (Source: Uniprot.org, NCBI)
|Protein Function||Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2- mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2 (PubMed:12944431).|
|Interacting Proteins||CCNA2; Ccna2; CCNB2; CCND1; CCNE1; CCNE2; CCNH; CDK7; CDKN1A; CDKN1B; CDKN3; CKS1B; EP300; IKBKG; LATS1; MAPK15; RB1; SCAPER; SCML2; STOML1; UHRF2|
|Research Areas||Breast Cancer; Cancer; Cell Cycle and Replication; Ovarian Carcinoma Cell Markers|
|Related Diseases||Malignant Neoplasms; Neoplasms; Retinoblastoma; Carcinoma; Malignant Neoplasm Of Breast; Mammary Neoplasms; Cell Transformation, Neoplastic; Growth Arrest; Hyperplasia; Leukemia; Malignant Paraganglionic Neoplasm; Prostatic Neoplasms; Liver Carcinoma; Malignant Neoplasm Of Prostate; Adenocarcinoma; Malignant Tumor Of Colon; Liver Neoplasms; Malignant Squamous Cell Neoplasm; Carcinogenesis|
|Related Pathways||Cell Cycle; Cell Proliferation; S Phase; Cell Cycle Arrest; Cell Growth; G1 Phase; Dna Replication; Cell Death; Mitosis; Localization; Cell Division; Induction Of Apoptosis; Hyperphosphorylation; Senescence; Cell Differentiation; Proteolysis; Centrosome Duplication; Protein Phosphorylation; Angiogenesis; Rna Interference|
|Background||CDK2, Cyclin-Dependent Kinase2, is also know as P33. The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34(cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.|
|Scientific References||PMID: 1714386 by Elledge S.J., et al. A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1.|
PMID: 1653904 by Tsai L.-H., et al. Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase.
Our Boster Quality Guarantee for Anti-Cyclin-dependent kinase 2 Cdk2 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti Cdk2 (PA1547) at 0.5ug/ml
WB: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 13
|Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug Targets. 2015;14(9):860-71. Platycodin D Induces Tumor Growth Arrest By Activating Foxo3A Expression In Prostate Cancer In Vitro And In Vivo.||PubMed ID 25431082|
|Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.||PubMed ID 25734831|
|Zhao P, Li Y, Gao G, Wang S, Yan Y, Zhan X, Liu Z, Mao Z, Chen S, Wang L. Eur J Med Chem. 2014 Oct 30;86:165-74. Doi: 10.1016/J.Ejmech.2014.08.049. Epub 2014 Aug 15. Design, Synthesis And Biological Evaluation Of N-Alkyl Or Aryl Substituted Isoind...||PubMed ID 25151579|
|Ye Z, Chen Z, Chen W, Xie J, Yang H, Lou Y, Yu Y. Chem Biol Interact. 2010 Jan 5;183(1):133-41. Doi: 10.1016/J.Cbi.2009.10.015. Xjw20, A Novel Oxoindole Derivative, Induces G2/M Arrest And Apoptosis Selectively In K562 Leukemia Cell Line.||PubMed ID 19883635|
|Cheng H, Chen C, Wang S, Ding G, Shi M. Diabetes Res Clin Pract. 2014 Mar;103(3):489-95. Doi: 10.1016/J.Diabres.2013.11.016. Epub 2013 Nov 28. The Effects Of Urokinase-Type Plasminogen Activator (Upa) On Cell Proliferation And Phenotypic Transform...||PubMed ID 24447806|
|Liu X, Lin X, Mi Y, Li J, Zhang C. Oxid Med Cell Longev. 2018 Jan 9;2018:9390810. doi: 10.1155/2018/9390810. eCollection 2018. Grape Seed Proanthocyanidin Extract Prevents Ovarian Aging by Inhibiting Oxidative Stress in the Hens||PubMed ID 29541349|
|TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells||PubMed ID 27065079|
|Effects of tripterygium glycosides on restenosis following endovascular treatment||PubMed ID 27108914|
|Rheum emodin inhibits enterovirus 71 viral replication and affects the host cell cycle environment||PubMed ID 27840410|
|Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D||PubMed ID 25659038|
|Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro||PubMed ID 27292614|
|Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 %u2026||PubMed ID 27432230|
|IL-2R%u03B1 up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell %u2026||PubMed ID 30340635|
Order Product (PA1547)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.