Anti-Cyclin-dependent kinase 2 Cdk2 Antibody

CDK2 antibody

Boster Bio Anti-Cyclin-dependent kinase 2 Cdk2 Antibody catalog # PA1547. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1547
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Cyclin-dependent kinase 2 Cdk2 Antibody

View all CDK2 Antibodies

SKU/Catalog Number

PA1547

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cyclin-dependent kinase 2 Cdk2 Antibody catalog # PA1547. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin-dependent kinase 2 Cdk2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1547)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cdk2 (277-298aa AKAALAHPFFQDVTKPVPHLRL), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1547 is reactive to CDK2 in Human, Mouse, Rat

Applications

PA1547 is guaranteed for WB Boster Guarantee

Background of CDK2

CDK2, Cyclin-Dependent Kinase2, is also know as P33. The CDK2 protein was highly homologous to p34 (CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34 (cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34 (cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34 (cdc2). P33 (cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For CDK2 (Source: Uniprot.org, NCBI)

Uniprot ID

P24941

Gene ID

1017

Gene Name

CDK2

Full Name

Cyclin-dependent kinase 2

Weight

33.93kDa

Superfamily

protein kinase superfamily

Alternative Names

cdc2-related protein kinase; CDK2; cell devision kinase 2; Cell division protein kinase 2; cyclin-dependent kinase 2; EC 2.7.11; EC 2.7.11.22; p33 protein kinase; p33(CDK2) CDK2 CDKN2, p33(CDK2) cyclin dependent kinase 2 cyclin-dependent kinase 2|cdc2-related protein kinase|cell division protein kinase 2|p33 protein kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDK2, check out the CDK2 Infographic

CDK2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PA1547 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The anti-tumor efficacy of 20(S)-protopanaxadiol, an active metabolite of ginseng, according to fasting on hepatocellular carcinoma

Wenzhen Li,Yifan Wang,Xinbo Zhou,Xiaohong Pan,Junhong Lü,Hongliu Sun,Zeping Xie,Shayan Chen,Xue Gao,The anti-tumor efficacy of 20(S)-protopanaxadiol, an active metabolite of ginseng, according to fasting on hepatocellular carcinoma,Journal of Ginseng Research,2021,,ISSN 1226-8453, https://doi.org/10.1016/j.jgr.2021.06.002.
Species: Human,Mouse
PA1547 usage in article: APP:WB, SAMPLE:HEPATOCARCINOMA CELL, DILUTION:1:1000

IL-2R%u03B1 up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell %u2026

Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 %u2026

Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro

Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D

Rheum emodin inhibits enterovirus 71 viral replication and affects the host cell cycle environment

Effects of tripterygium glycosides on restenosis following endovascular treatment

TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells

Liu X, Lin X, Mi Y, Li J, Zhang C. Oxid Med Cell Longev. 2018 Jan 9;2018:9390810. doi: 10.1155/2018/9390810. eCollection 2018. Grape Seed Proanthocyanidin Extract Prevents Ovarian Aging by Inhibiting Oxidative Stress in the Hens

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4 Customer Q&As for Anti-Cyclin-dependent kinase 2 Cdk2 Antibody

Question

My team were happy with the WB result of your anti-Cdk2 antibody. However we have been able to see positive staining in left lobe of thyroid gland microtubule using this antibody. Is that expected? Could you tell me where is CDK2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

From literature, left lobe of thyroid gland does express CDK2. Generally CDK2 expresses in cytoplasm, cytoskeleton, microtubule. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-09-17

Question

We have observed staining in mouse cervix carcinoma. Any tips? Is anti-Cdk2 antibody supposed to stain cervix carcinoma positively?

A. Anderson

Verified customer

Asked: 2018-11-21

Answer

From literature cervix carcinoma does express CDK2. From Uniprot.org, CDK2 is expressed in left lobe of thyroid gland, placenta, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-11-21

Question

We are currently using anti-Cdk2 antibody PA1547 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2018-08-07

Answer

The anti-Cdk2 antibody (PA1547) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-07

Question

We want using your anti-Cdk2 antibody for cell division studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-02-05

Answer

We appreciate your inquiry. This PA1547 anti-Cdk2 antibody is validated on jurkat whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-02-05

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Total: $315

SKU:PA1547

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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