Cyclin E1 Colorimetric Cell-Based ELISA Kit

Cyclin E1 ELISA kit, cell-based

The Cyclin E1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Cyclin E1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Cyclin E1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Cyclin E1.

Product Info Summary

SKU: EKC1156
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

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Product Name

Cyclin E1 Colorimetric Cell-Based ELISA Kit

See all Cyclin E1 products

SKU/Catalog Number

EKC1156

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The Cyclin E1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Cyclin E1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Cyclin E1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Cyclin E1.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

Cyclin E1 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1156)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1156 is reactive to CCNE1 in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1156 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Cyclin E1 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For CCNE1 (Source: Uniprot.Org, NCBI)

Gene Name

CCNE1

Full Name

G1/S-specific cyclin-E1

Weight

47077 MW

Superfamily

cyclin family

Alternative Names

CCNE;CCNE1;G1/S-specific cyclin E1 CCNE1 CCNE, pCCNE1 cyclin E1 G1/S-specific cyclin-E1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCNE1, check out the CCNE1 Infographic

CCNE1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCNE1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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