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Product Info Summary
| SKU: | PA1547 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cyclin-dependent kinase 2 Cdk2 Antibody Picoband®
SKU/Catalog Number
PA1547
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cyclin-dependent kinase 2 Cdk2 Antibody catalog # PA1547. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin-dependent kinase 2 Cdk2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1547)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cdk2, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1547 is reactive to CDK2 in Human, Mouse, Rat
Observed Molecular Weight
30 kDa
Calculated molecular weight
33.9 kDa
Background of CDK2
CDK2, Cyclin-Dependent Kinase2, is also know as P33. The CDK2 protein was highly homologous to p34 (CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34 (cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34 (cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34 (cdc2). P33 (cdk2) plays a unique role in cell cycle regulation of vertebrate cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1547 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human Jurkat whole cell lysates, human HepG2 whole cell lysates, human U2OS whole cell lysates, human K562 whole cell lysates, human T47D whole cell lysates, human CACO-2 whole cell lysates, human 293T whole cell lysates, human PC-3 whole cell lysates, rat L6 whole cell, mouse NIH/3T3 whole cell.
Validation Images & Assay Conditions
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Western blot analysis of CDK2 using antiCDK2 antibody (PA1547).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human T47D whole cell lysates,
Lane 6: human CACO-2 whole cell lysates,
Lane 7: human 293T whole cell lysates,
Lane 8: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK2 antigen affinity purified polyclonal antibody (Catalog # PA1547) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK2 at approximately 30 kDa. The expected band size for CDK2 is at 30, 34 kDa.
Click image to see more details
Western blot analysis of CDK2 using antiCDK2 antibody (PA1547).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat L6 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK2 antigen affinity purified polyclonal antibody (Catalog # PA1547) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK2 at approximately 30 kDa. The expected band size for CDK2 is at 30, 34 kDa.
Specific Publications For Anti-Cyclin-dependent kinase 2 Cdk2 Antibody Picoband® (PA1547)
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4 Customer Q&As for Anti-Cyclin-dependent kinase 2 Cdk2 Antibody Picoband®
Question
My team were happy with the WB result of your anti-Cdk2 antibody. However we have been able to see positive staining in left lobe of thyroid gland microtubule using this antibody. Is that expected? Could you tell me where is CDK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
From literature, left lobe of thyroid gland does express CDK2. Generally CDK2 expresses in cytoplasm, cytoskeleton, microtubule. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-17
Question
We have observed staining in mouse cervix carcinoma. Any tips? Is anti-Cdk2 antibody supposed to stain cervix carcinoma positively?
A. Anderson
Verified customer
Asked: 2018-11-21
Answer
From literature cervix carcinoma does express CDK2. From Uniprot.org, CDK2 is expressed in left lobe of thyroid gland, placenta, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-11-21
Question
We are currently using anti-Cdk2 antibody PA1547 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-07
Answer
The anti-Cdk2 antibody (PA1547) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-07
Question
We want using your anti-Cdk2 antibody for cell division studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
We appreciate your inquiry. This PA1547 anti-Cdk2 antibody is validated on jurkat whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-02-05
