Anti-CEBP Beta/CEBPB Antibody Picoband™

CEBP Beta antibody

Boster Bio Anti-CEBP Beta/CEBPB Antibody Picoband™ catalog # PB9171. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9171
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, IHC-F, ICC, WB

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Product Name

Anti-CEBP Beta/CEBPB Antibody Picoband™

View all CEBP Beta Antibodies

SKU/Catalog Number

PB9171

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CEBP Beta/CEBPB Antibody Picoband™ catalog # PB9171. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CEBP Beta/CEBPB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9171)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CEBP Beta recombinant protein (Position: M1-A200). Human CEBP Beta shares 61% amino acid (aa) sequence identity with both mouse and rat CEBP Beta.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9171 is reactive to CEBPB in Human, Mouse, Rat

Applications

PB9171 is guaranteed for IF, IHC, IHC-F, ICC, WB Boster Guarantee

Background of CEBP Beta

CCAAT/enhancer-binding protein beta, also known as is CEBP-beta, is a protein that in humans is encoded by the CEBPB gene. It mapped to 20q13.13. The protein encoded by this intronless gene is a bZIP transcription factor that can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. CEBP-beta is critical for normal macrophage functioning, an important immune cell sub-type. Observational work has shown that expression of CEBP-beta in blood leukocytes is positively associated with muscle strength in humans, emphasizing the importance of the immune system, and particularly macrophages, in the maintenance of muscle function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CEBPB (Source: Uniprot.org, NCBI)

Uniprot ID

P17676

Gene ID

1051

Gene Name

CEBPB

Full Name

CCAAT/enhancer-binding protein beta

Weight

36.106kDa

Superfamily

bZIP family

Alternative Names

C/EBP beta; C/EBP-beta; CCAAT/enhancer binding protein (C/EBP), beta; CCAAT/enhancer-binding protein beta; CRP2; IL6DBP; interleukin 6-dependent DNA-binding protein; LAPTCF-5; Liver activator protein; liver-enriched transcriptional activator protein; MGC32080; NF-IL6; Nuclear factor NF-IL6; nuclear factor of interleukin 6; TCF5NFIL6; Transcription factor 5 CEBPB C/EBP-beta, IL6DBP, NF-IL6, TCF5 CCAAT enhancer binding protein beta CCAAT/enhancer-binding protein beta|CCAAT/enhancer binding protein (C/EBP), beta|interleukin 6-dependent DNA-binding protein|nuclear factor NF-IL6|nuclear factor of interleukin 6|transcription factor 5|transcription factor C/EBP beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CEBPB, check out the CEBPB Infographic

CEBPB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CEBPB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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1 Customer Q&As for Anti-CEBP Beta/CEBPB Antibody Picoband™

Question

We are currently using anti-CEBP Beta/CEBPB antibody PB9171 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

The anti-CEBP Beta/CEBPB antibody (PB9171) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-10

Size

Conjugation

Total: $315

SKU:PB9171

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PB9171
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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