CEBPB Antibodies

AND CEBPB ELISA kits, CEBPB Proteins

Many biological assays use antibodies to detect CEBPB, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with CEBPB in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop CEBPB antibodies...

We validate the specificity of these antibodies to CEBPB by testing them on tissues known to express CEBPB positively and negatively. Browse below to find the CEBPB antibody that suites your experiment. We have 9 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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CEBPB Infographic by Boster Bio

All CEBPB Products

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Applications

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Anti-CEBP Beta/CEBPB Antibody Picoband®

3 Citations 1 Q&As

Western blot analysis of CEBP Beta using anti-CEBP Beta antibody (PB9171)

IHC analysis of CEBP Beta using anti-CEBP Beta antibody (PB9171)

Human

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # PB9171
100 μg/vial

Anti-CEBP Beta Rabbit Monoclonal Antibody

1 Citations 3 Q&As

Western blot analysis of CEBP Beta in (1) PC-12 cell lysate; (2) Human spleen lysate

Human, Mouse, Rat

Flow Cytometry, IP, WB

$370
Cat # M01100-1
100 μl

Anti-CEBP beta Rabbit Monoclonal Antibody

1 Citations

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature

Western blot analysis of CEBP beta expression in MCF-7 cell lysate

Immunofluorescent analysis of Hela cells, using CEBP beta Antibody

Human, Mouse, Rat

Flow Cytometry, IP, IF, ICC, WB

$370
Cat # M01100
100 μl

CEBPB Colorimetric Cell-Based ELISA

Western blot analysis of extracts from Jurkat cells

Human, Mouse

ELISA

$480
Cat # EKC1683
1 kit, containing one 96-well plate and all necessary reagents

Anti-C/EBP-Beta (S229/182) CEBPB Antibody

Immunohistochemistry (IHC) analyzes of C/EBP-β (S229/182) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IHC

$405
Cat # A01100S229
100ul

Anti-C/EBP-Beta (phospho-T235/188) CEBPB Antibody

Western blot (WB) analysis of C/EBP-β (phospho-T235/188) pAb at 1:500 dilution
Lane1:MCF-7 treated with insulin(500ng/ml,5min) whole cell lysate(40ug)
Lane2:MCF-7 treated with insulin(500ng/ml,0min) whole cell lysate (40ug)

Human

IHC, WB

$482
Cat # A01100T235
100ul

Anti-C/EBP Beta CEBPB Antibody

Western blot analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1,000 dilution

ICC staining C/EBP Beta in Hela cells (green)

Human, Mouse, Rat

Flow Cytometry, IP, IF, ICC, WB

$405
Cat # A01100-1
100ul

CEBP Beta (CEBPB) (NM_005194) Human Over-expression Lysate

Human

$628
Cat # LS001051
20 µg, 100 µg

Anti-Phospho-CEBPB (T235/T188) Antibody

Human, Mouse, Rat

IF, IHC, ICC, WB

$370
Cat # P01100
100 μl/vial

CEBPB Overview

Facts about CCAAT/enhancer-binding protein beta.

CEBPB 3D structure. Source: alphafold

CCAAT/enhancer-binding protein beta (CEBPB)

Significant transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:1741402, PubMed:9374525, PubMed:12048245, PubMed:18647749). Plays also a significant role in adipogenesis, in addition to from the gluconeogenic pathway, liver regeneration, and hematopoiesis.

Its functional capacity is regulated by protein interactions and post-translational protein alterations. During early embryogenesis, plays essential and redundant functions with CEBPA.

Gene Information

Human
Gene Name:	CEBPB
Uniprot:	P17676
Entrez:	1051

Family

Belongs to:

bZIP family

Alternative Names

C/EBP beta; C/EBP-beta; CCAAT/enhancer binding protein (C/EBP), beta; CCAAT/enhancer-binding protein beta; CRP2; IL6DBP; interleukin 6-dependent DNA-binding protein; LAPTCF-5; Liver activator protein; liver-enriched transcriptional activator protein; MGC32080; NF-IL6; Nuclear factor NF-IL6; nuclear factor of interleukin 6; TCF5NFIL6; Transcription factor 5

Molecular Weight

Mass (kDA):
36.106 kDA

Genomic Context


Human

Location:	20q13.13
Sequence:	20; NC_000020.11 (50190583..50192690)

Tissue Specificity

Expressed at low levels in the lung, kidney and spleen.

Subcellular Localizations

Nucleus. Cytoplasm. Translocates to the nucleus when phosphorylated at Ser-288. In T-cells when sumoylated drawn to pericentric heterochromatin thereby allowing proliferation (By similarity).

Diseases

Neoplasms
Malignant Neoplasms
Leukemia
Inflammation
Myeloid Leukemia
Leukemia, Myelocytic, Acute
Lymphoma
Cell Transformation, Neoplastic
Diabetes Mellitus
Malignant Paraganglionic Neoplasm
Obesity
Carcinoma

Malignant Neoplasm Of Breast
Diabetes Mellitus, Non-insulin-dependent
Inflammatory Response
Acute Leukemia
Leukemogenesis
Tissue Adhesions
Acute Lymphocytic Leukemia

Interacting Proteins

ATF4
BATF
CARM1
CEBPA
CEBPG

DDIT3
ESR1
MUC1
SUMO1
TP53

Pathways

Cell Cycle
Cell Proliferation
Immune Response
Cell Differentiation
Translation
Senescence
Regeneration
Oncogene-induced Senescence
Pathogenesis
Inflammatory Response
Cell Death
Cell Growth
Rna Interference

Ovulation
Angiogenesis
Luteinization
Secretion
Osteoblast Differentiation
Gluconeogenesis
Embryo Implantation

PTMs

Phosphorylation
Acetylation
Methylation

Sumoylation
Dephosphorylation
Oxidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CEBPB

References

PMID: 2112087 by Akira S., et al. A nuclear factor for IL-6 expression (NF-IL6) is a member of a C/EBP family.

PMID: 7635140 by Toda K., et al. Identification of a transcriptional regulatory factor for human aromatase cytochrome P450 gene expression as nuclear factor interleukin-6 (NF-IL6), a member of the CCAAT/enhancer-binding protein family.