Anti-Phospho-Cenexin-1 S796 ODF2 Antibody

Product Name

Anti-Phospho-Cenexin-1 S796 ODF2 Antibody

View all ODF2 Antibodies

SKU/Catalog Number

P05599

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-Cenexin-1 S796 ODF2 Antibody (Catalog # P05599). Tested in ELISA, WB applications. This antibody reacts with Human, Chimpanzee, Macaque Monkey.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Phospho-Cenexin-1 S796 ODF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P05599)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near S796 of human cenexin-1.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P05599 is reactive to ODF2 in Human, Chimpanzee, Macaque monkey

Observed Molecular Weight

42 kDa

Calculated molecular weight

95.4 kDa

Background of ODF2

Cenexin-1, also known as ODF2 and outer dense fiber of sperm tails 2, are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene; however, not all isoforms and variants have been fully described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P05599 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: User optimized
WB: 1:200 - 1:2,000
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 93 kDa in size corresponding to phosphorylated Cenexin-1 by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with human Cenexin-1 pS796 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Validation Images & Assay Conditions

See full target information ODF2

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