Anti-Chromogranin B/CHGB Antibody

Product Name

Anti-Chromogranin B/CHGB Antibody

View all CHGB Antibodies

SKU/Catalog Number

A04073-3

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-Chromogranin B/CHGB Antibody catalog # A04073-3. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Cite This Product

Anti-Chromogranin B/CHGB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04073-3)

Host

Rabbit

Contents

500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Immunogen

E.coli-derived human Chromogranin B/CHGB recombinant protein (Position: T330-S674).

Reactive Species

A04073-3 is reactive to CHGB in Human, Mouse, Rat

Calculated molecular weight

78.3 kDa

Background of CHGB

Chromogranin B is also named as Secretogranin-1, SgI, CgB, CHGB and SCG1. CHGB, a secretory granule protein, inserts itself into membrane and forms a chloride-conducting channel (PMID: 30456382). Native CHGB distributes nearly equally in soluble and membrane-bound forms, and both reconstitute highly selective anion channels in membrane (PMID: 37435575). The calculated molecular weight of Chromogranin B is at 78kDa. It has been shown that CHGB has a clear degradation band at ~50 kDa (https://www.biorxiv.org/content/10.1101/2019.12.28.890053v1.full.pdf).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04073-3 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunofluorescence, 1:50-400
ELISA, 1:100-1000

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information CHGB

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