Anti-Cleaved-CD9 beta (S531) ADGRE5 Antibody

Product Name

Anti-Cleaved-CD9 beta (S531) ADGRE5 Antibody

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SKU/Catalog Number

A30392S531

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Cleaved-CD9 beta (S531) ADGRE5 Antibody catalog # A30392S531. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cleaved-CD9 beta (S531) ADGRE5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30392S531)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human CD97beta

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30392S531 is reactive to in Human

Applications

A30392S531 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

51012 MW

Background of

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. ORF57 (also known as MTA), one of the earliest Kaposi's sarcoma-associated herpesvirus (KSHV) regulatory proteins to be expressed, is essential for virus lytic replication. A counterpart is present in every herpesvirus sequenced, indicating the importance of this signature viral protein, and those examined act post-transcriptionally, affecting RNA splicing and transport. KSHV ORF57 is capable of establishing both lytic and latent replication cycles.  In KS, the virus localizes to tumor progenitor endothelial cells, most of which are latently infected. In cell culture, KSHV replication is generally studied using B-cell lines, such as BCBL-1, generated from primary effusion lymphoma material. Most BCBL-1 cells are latently infected, although there is some spontaneous virus reactivation. Addition of chemical inducers such as sodium n-butyrate, 12-O-tetradecanoylphorbol-13-acetate (TPA), and valproic acid (VA) to these cells efficiently induces the lytic cycle and produces virions. KSHV ORF57 protein predominantly localizes to the nucleus and can shuttle between the nucleus and cytoplasm. Most HSV-1 genes are unspliced; by contrast, ORF57 is spliced gene; the protein is 455 amino acids in length and 50kDa in size.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ELISA 1:5000

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

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