Overview
| Product Name |
Anti-Cleaved-Notch 1 (V1754) Antibody
See more NOTCH1 products |
| Catalog# |
A00033-1 |
| Pack Size |
100μl |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-Cleaved-Notch 1 (V1754) Antibody catalog # A00033-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μl in Liquid form antibody. |
| Cite This Product |
Anti-Cleaved-Notch 1 (V1754) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00033-1)
|
| Antibodies Validation |
Antibodies Validation Information
|
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Anti-Cleaved-Notch 1 (V1754) Antibody may replace the following items: sc 12825|sc 271285|sc 10783|sc 32736|sc 5342|sc 28713|sc 271128|sc 55019|sc 55021|sc 55020|sc 8213|sc 28713 X|sc 8213 X|sc 32636|sc 377399|sc 8643|sc 8646|sc 8645|sc 5594|sc 32612|sc 32613|sc 32634|sc 8644|sc 393893|sc 7424|sc 5593|sc 32347|sc 32346|sc 32344|sc 5545|sc 7423|sc 23304|sc 23299|sc 9170|sc 23301|sc 373944|sc 6014|sc 373891|sc 376403|sc 6015|sc 32756|sc 71719|sc 32745|sc 6014 R|sc 6015 R|sc 5540|sc 17752|sc 1087|sc 5540 X|sc 23307|sc 7091|sc 7093|sc 9161|sc 52454. |
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human, Mouse, Rat |
| Applications |
WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
Synthesized peptide derived from the Internal region of human Notch 1. at AA rangle: 1710-1790 |
Gene/Protein Basic Information For NOTCH1 (Source: Uniprot.org, NCBI)
| Uniprot Id | P46531 |
|---|
| NCBI Gene Id | 4851 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | NOTCH1 |
|---|
| Protein Name | Neurogenic locus notch homolog protein 1 |
|---|
| Superfamily | NOTCH family |
|---|
| Alternative Names | NOTCH1|EC 2.1.2.11; EC 3.4.21.68; hN1; Notch (Drosophila) homolog 1 (translocation-associated); notch 1Notch homolog 1, translocation-associated (Drosophila); Notch homolog 1, translocation-associated; Notch1; Notch-1; TAN1; TAN1neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1 |
|---|
| Molecular Weight | 272505 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on NOTCH1, check out the NOTCH1 Infographic
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In this infographic you will see the following information for NOTCH1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the NOTCH1 infographic
Our Boster Quality Guarantee for Anti-Cleaved-Notch 1 (V1754) Antibody covers its use in the following applications.
WB, 1:500-1:2000
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Western Blot (WB) analysis of KB cells using Cleaved-Notch 1 (V1754) Polyclonal antibody.
Western Blot (WB) analysis of specific cells using Cleaved-Notch 1 (V1754) Polyclonal antibody.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
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Total number of citations: 0
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Questions and answers from customers related to A00033-1 Anti-Cleaved-Notch 1 (V1754) Antibody
15 Related Questions
Question
I was wanting to use your anti-Cleaved-Notch 1 (V1754) antibody for WB for mouse visceral pleura on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse visceral pleura identification?
V. Bhatt
Asked: 2020-03-23
Answer
You can see on the product datasheet, A00033-1 anti-Cleaved-Notch 1 (V1754) antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse visceral pleura in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-23
Question
Would anti-Cleaved-Notch 1 (V1754) antibody A00033-1 work for WB with visceral pleura?
Verified Customer
Asked: 2020-02-13
Answer
According to the expression profile of visceral pleura, NOTCH1 is highly expressed in visceral pleura. So, it is likely that anti-Cleaved-Notch 1 (V1754) antibody A00033-1 will work for WB with visceral pleura.
Boster Scientific Support
Answered: 2020-02-13
Question
We have observed staining in mouse aortic endothelium. Are there any suggestions? Is anti-Cleaved-Notch 1 (V1754) antibody supposed to stain aortic endothelium positively?
Verified Customer
Asked: 2020-01-30
Answer
From literature aortic endothelium does express NOTCH1. From Uniprot.org, NOTCH1 is expressed in visceral pleura, brain, aortic endothelium, cervix carcinoma, among other tissues. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648
Boster Scientific Support
Answered: 2020-01-30
Question
I see that the anti-Cleaved-Notch 1 (V1754) antibody A00033-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Asked: 2019-12-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-23
Question
you antibody using your anti-Cleaved-Notch 1 (V1754) antibody for negative regulation of cell migration involved in sprouting angiogenesis studies. Has this antibody been tested with western blotting on kb cells? We would like to see some validation images before ordering.
Verified Customer
Asked: 2019-11-28
Answer
Thank you for your inquiry. This A00033-1 anti-Cleaved-Notch 1 (V1754) antibody is validated on kb cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-28
Question
Does A00033-1 anti-Cleaved-Notch 1 (V1754) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Asked: 2019-07-09
Answer
It shows on the product datasheet, A00033-1 anti-Cleaved-Notch 1 (V1754) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-09
Question
See below the WB image, lot number and protocol we used for visceral pleura using anti-Cleaved-Notch 1 (V1754) antibody A00033-1. Please let me know if you require anything else.
Verified Customer
Asked: 2019-07-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-04
Question
My lab would like to test anti-Cleaved-Notch 1 (V1754) antibody A00033-1 on mouse visceral pleura for research purposes, then I may be interested in using anti-Cleaved-Notch 1 (V1754) antibody A00033-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Asked: 2019-06-04
Answer
The products we sell, including anti-Cleaved-Notch 1 (V1754) antibody A00033-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-04
Question
Is a blocking peptide available for product anti-Cleaved-Notch 1 (V1754) antibody (A00033-1)?
Verified Customer
Asked: 2019-06-03
Answer
We do provide the blocking peptide for product anti-Cleaved-Notch 1 (V1754) antibody (A00033-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-03
Question
We are currently using anti-Cleaved-Notch 1 (V1754) antibody A00033-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Asked: 2019-03-12
Answer
The anti-Cleaved-Notch 1 (V1754) antibody (A00033-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-12
Question
Is this A00033-1 anti-Cleaved-Notch 1 (V1754) antibody reactive to the isotypes of NOTCH1?
D. Roberts
Asked: 2018-06-06
Answer
The immunogen of A00033-1 anti-Cleaved-Notch 1 (V1754) antibody is Synthesized peptide derived from the Internal region of human Notch 1. at AA rangle: 1710-1790. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-06
Question
Can you help my question with product A00033-1, anti-Cleaved-Notch 1 (V1754) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Asked: 2018-01-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00033-1 anti-Cleaved-Notch 1 (V1754) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-08
Question
Our team were satisfied with the WB result of your anti-Cleaved-Notch 1 (V1754) antibody. However we have observed positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is NOTCH1 supposed to be expressed?
M. Mangal
Asked: 2014-03-25
Answer
From literature, cervix carcinoma does express NOTCH1. Generally NOTCH1 expresses in cell membrane, notch 1 intracellular domain: nucleus. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648
Boster Scientific Support
Answered: 2014-03-25
Question
Do you have a BSA free version of anti-Cleaved-Notch 1 (V1754) antibody A00033-1 available?
P. Zhang
Asked: 2014-03-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cleaved-Notch 1 (V1754) antibody A00033-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-03-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for visceral pleura using anti-Cleaved-Notch 1 (V1754) antibody A00033-1. Let me know if you need anything else.
P. Brown
Asked: 2013-11-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-29
