Anti-Cleaved-Notch 1 (V1754) Antibody

Boster Bio Anti-Cleaved-Notch 1 (V1754) Antibody catalog # A00033-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00033-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Cleaved-Notch 1 (V1754) Antibody

See all NOTCH1 products

SKU/Catalog Number

A00033-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Cleaved-Notch 1 (V1754) Antibody catalog # A00033-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cleaved-Notch 1 (V1754) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00033-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human Notch 1. at AA rangle: 1710-1790

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00033-1 is reactive to NOTCH1 in Human, Mouse, Rat

Applications

A00033-1 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For NOTCH1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P46531

Gene ID

4851

Gene Name

NOTCH1

Full Name

Neurogenic locus notch homolog protein 1

Weight

272.505kDa

Superfamily

NOTCH family

Alternative Names

EC 2.1.2.11; EC 3.4.21.68; hN1; Notch (Drosophila) homolog 1 (translocation-associated); notch 1Notch homolog 1, translocation-associated (Drosophila); Notch homolog 1, translocation-associated; Notch1; Notch-1; TAN1; TAN1neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1 NOTCH1 AOS5, AOVD1, TAN1, hN1 notch receptor 1 neurogenic locus notch homolog protein 1|Notch homolog 1, translocation-associated|notch 1|translocation-associated notch protein TAN-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NOTCH1, check out the NOTCH1 Infographic

NOTCH1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NOTCH1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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15 Customer Q&As for Anti-Cleaved-Notch 1 (V1754) Antibody

Question

I was wanting to use your anti-Cleaved-Notch 1 (V1754) antibody for WB for mouse visceral pleura on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse visceral pleura identification?

V. Bhatt

Verified customer

Asked: 2020-03-23

Answer

You can see on the product datasheet, A00033-1 anti-Cleaved-Notch 1 (V1754) antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse visceral pleura in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-23

Question

Would anti-Cleaved-Notch 1 (V1754) antibody A00033-1 work for WB with visceral pleura?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

According to the expression profile of visceral pleura, NOTCH1 is highly expressed in visceral pleura. So, it is likely that anti-Cleaved-Notch 1 (V1754) antibody A00033-1 will work for WB with visceral pleura.

Boster Scientific Support

Answered: 2020-02-13

Question

We have observed staining in mouse aortic endothelium. Are there any suggestions? Is anti-Cleaved-Notch 1 (V1754) antibody supposed to stain aortic endothelium positively?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

From literature aortic endothelium does express NOTCH1. From Uniprot.org, NOTCH1 is expressed in visceral pleura, brain, aortic endothelium, cervix carcinoma, among other tissues. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648

Boster Scientific Support

Answered: 2020-01-30

Question

I see that the anti-Cleaved-Notch 1 (V1754) antibody A00033-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-23

Question

you antibody using your anti-Cleaved-Notch 1 (V1754) antibody for negative regulation of cell migration involved in sprouting angiogenesis studies. Has this antibody been tested with western blotting on kb cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

Thank you for your inquiry. This A00033-1 anti-Cleaved-Notch 1 (V1754) antibody is validated on kb cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-28

Question

Does A00033-1 anti-Cleaved-Notch 1 (V1754) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

It shows on the product datasheet, A00033-1 anti-Cleaved-Notch 1 (V1754) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-09

Question

See below the WB image, lot number and protocol we used for visceral pleura using anti-Cleaved-Notch 1 (V1754) antibody A00033-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-04

Question

My lab would like to test anti-Cleaved-Notch 1 (V1754) antibody A00033-1 on mouse visceral pleura for research purposes, then I may be interested in using anti-Cleaved-Notch 1 (V1754) antibody A00033-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

The products we sell, including anti-Cleaved-Notch 1 (V1754) antibody A00033-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-04

Question

Is a blocking peptide available for product anti-Cleaved-Notch 1 (V1754) antibody (A00033-1)?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

We do provide the blocking peptide for product anti-Cleaved-Notch 1 (V1754) antibody (A00033-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-03

Question

We are currently using anti-Cleaved-Notch 1 (V1754) antibody A00033-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-03-12

Answer

The anti-Cleaved-Notch 1 (V1754) antibody (A00033-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-03-12

Question

Is this A00033-1 anti-Cleaved-Notch 1 (V1754) antibody reactive to the isotypes of NOTCH1?

D. Roberts

Verified customer

Asked: 2018-06-06

Answer

The immunogen of A00033-1 anti-Cleaved-Notch 1 (V1754) antibody is Synthesized peptide derived from the Internal region of human Notch 1. at AA rangle: 1710-1790. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-06-06

Question

Can you help my question with product A00033-1, anti-Cleaved-Notch 1 (V1754) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-01-08

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00033-1 anti-Cleaved-Notch 1 (V1754) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-01-08

Question

Our team were satisfied with the WB result of your anti-Cleaved-Notch 1 (V1754) antibody. However we have observed positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is NOTCH1 supposed to be expressed?

M. Mangal

Verified customer

Asked: 2014-03-25

Answer

From literature, cervix carcinoma does express NOTCH1. Generally NOTCH1 expresses in cell membrane, notch 1 intracellular domain: nucleus. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648

Boster Scientific Support

Answered: 2014-03-25

Question

Do you have a BSA free version of anti-Cleaved-Notch 1 (V1754) antibody A00033-1 available?

P. Zhang

Verified customer

Asked: 2014-03-24

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cleaved-Notch 1 (V1754) antibody A00033-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-03-24

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for visceral pleura using anti-Cleaved-Notch 1 (V1754) antibody A00033-1. Let me know if you need anything else.

P. Brown

Verified customer

Asked: 2013-11-29

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-11-29

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A00033-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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