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Product Info Summary
| SKU: | A00033-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Cleaved-Notch 1 (V1754) Antibody
SKU/Catalog Number
A00033-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cleaved-Notch 1 (V1754) Antibody catalog # A00033-1. Tested in WB, IF, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cleaved-Notch 1 (V1754) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00033-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Notch 1. AA range:1735-1784
Reactive Species
A00033-1 is reactive to NOTCH1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
272.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00033-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
IF 1:50-300
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Cleaved-Notch 1 (V1754) Polyclonal Antibody diluted at 1:500
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Western Blot analysis of KB cells using Cleaved-Notch 1 (V1754) Polyclonal Antibody diluted at 1:500
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Immunofluorescence analysis of Hela cell. 1,Cleaved-Notch 1 (V1754) Polyclonal Antibody (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
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Immunofluorescence analysis of Human-lung-cancer tissue. 1,Cleaved-Notch 1 (V1754) Polyclonal Antibody (red) was diluted at 1:200 (4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Specific Publications For Anti-Cleaved-Notch 1 (V1754) Antibody (A00033-1)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Cleaved-Notch 1 (V1754) Antibody
Question
I was wanting to use your anti-Cleaved-Notch 1 (V1754) antibody for WB for mouse visceral pleura on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse visceral pleura identification?
V. Bhatt
Verified customer
Asked: 2020-03-23
Answer
You can see on the product datasheet, A00033-1 anti-Cleaved-Notch 1 (V1754) antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse visceral pleura in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-23
Question
Would anti-Cleaved-Notch 1 (V1754) antibody A00033-1 work for WB with visceral pleura?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
According to the expression profile of visceral pleura, NOTCH1 is highly expressed in visceral pleura. So, it is likely that anti-Cleaved-Notch 1 (V1754) antibody A00033-1 will work for WB with visceral pleura.
Boster Scientific Support
Answered: 2020-02-13
Question
We have observed staining in mouse aortic endothelium. Are there any suggestions? Is anti-Cleaved-Notch 1 (V1754) antibody supposed to stain aortic endothelium positively?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
From literature aortic endothelium does express NOTCH1. From Uniprot.org, NOTCH1 is expressed in visceral pleura, brain, aortic endothelium, cervix carcinoma, among other tissues. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648
Boster Scientific Support
Answered: 2020-01-30
Question
I see that the anti-Cleaved-Notch 1 (V1754) antibody A00033-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-23
Question
you antibody using your anti-Cleaved-Notch 1 (V1754) antibody for negative regulation of cell migration involved in sprouting angiogenesis studies. Has this antibody been tested with western blotting on kb cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
Thank you for your inquiry. This A00033-1 anti-Cleaved-Notch 1 (V1754) antibody is validated on kb cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-28
Question
Does A00033-1 anti-Cleaved-Notch 1 (V1754) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
It shows on the product datasheet, A00033-1 anti-Cleaved-Notch 1 (V1754) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-09
Question
See below the WB image, lot number and protocol we used for visceral pleura using anti-Cleaved-Notch 1 (V1754) antibody A00033-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-04
Question
My lab would like to test anti-Cleaved-Notch 1 (V1754) antibody A00033-1 on mouse visceral pleura for research purposes, then I may be interested in using anti-Cleaved-Notch 1 (V1754) antibody A00033-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
The products we sell, including anti-Cleaved-Notch 1 (V1754) antibody A00033-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-04
Question
Is a blocking peptide available for product anti-Cleaved-Notch 1 (V1754) antibody (A00033-1)?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
We do provide the blocking peptide for product anti-Cleaved-Notch 1 (V1754) antibody (A00033-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-03
Question
We are currently using anti-Cleaved-Notch 1 (V1754) antibody A00033-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
The anti-Cleaved-Notch 1 (V1754) antibody (A00033-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-12
Question
Is this A00033-1 anti-Cleaved-Notch 1 (V1754) antibody reactive to the isotypes of NOTCH1?
D. Roberts
Verified customer
Asked: 2018-06-06
Answer
The immunogen of A00033-1 anti-Cleaved-Notch 1 (V1754) antibody is Synthesized peptide derived from the Internal region of human Notch 1. at AA rangle: 1710-1790. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-06
Question
Can you help my question with product A00033-1, anti-Cleaved-Notch 1 (V1754) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00033-1 anti-Cleaved-Notch 1 (V1754) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-08
Question
Our team were satisfied with the WB result of your anti-Cleaved-Notch 1 (V1754) antibody. However we have observed positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is NOTCH1 supposed to be expressed?
M. Mangal
Verified customer
Asked: 2014-03-25
Answer
From literature, cervix carcinoma does express NOTCH1. Generally NOTCH1 expresses in cell membrane, notch 1 intracellular domain: nucleus. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648
Boster Scientific Support
Answered: 2014-03-25
Question
Do you have a BSA free version of anti-Cleaved-Notch 1 (V1754) antibody A00033-1 available?
P. Zhang
Verified customer
Asked: 2014-03-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cleaved-Notch 1 (V1754) antibody A00033-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-03-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for visceral pleura using anti-Cleaved-Notch 1 (V1754) antibody A00033-1. Let me know if you need anything else.
P. Brown
Verified customer
Asked: 2013-11-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-29
