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Col2a1 antibody (PA2141-1) performs well in IF, IHC, WB and shows reactivity with Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 142 Publications

pa2141 1 col2a1 primary antibodies if testing 1

IF analysis of COL2A1 using anti-COL2A1 antibody (PA2141-1).
COL2A1 was detected in a paraffin-embedded section of Mouse cartilage tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-COL2A1 Antibody (PA2141-1) overnight at 4°C. Donkey anti-rabbit Alexa Fluor 555was used as secondary antibody at 1:500 dilution and incubated for 1 hour at RT. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-Collagen II/COL2A1 Antibody Picoband®

Name: Anti-Collagen II/COL2A1 Antibody Picoband®
Brand: Boster Bio
SKU: PA2141-1
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

Col2a1 antibody

142 Publications|

1 Review

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Reactive Species	Host	Application
Mouse, Rat	Rabbit	IF, IHC, WB

Tested Applications

Col2a1 antibody was positively detected by WB in:

Rat Trachea Tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Col2a1 antibody was positively detected by IF in:

Mouse cartilage tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Rat

Tested

Recommend

Mouse

Recommend

Tested

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Human

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-Collagen II/COL2A1 Antibody Picoband®
View all COL2A1 Antibodies

Form

Lyophilized

Uniprot

P28481

Gene ID

12824

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen II1222-1241aa AFAGLGQREKGPDPMQYMRA), different from the related rat sequence by one amino acid.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

170 kDa

Calculated Molecular Weight

142.0 kDa

Background of Col2a1

Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Cite This Product

Anti-Collagen II/COL2A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2141-1)

Protocols

Western Blot protocol for Col2a1 antibody

Review Protocol

Immunofluorescence (IF) protocol for Col2a1 antibody

Review Protocol

Publications

Inhibition of PARP-1 attenuates osteoarthritis progression by suppressing NF-κB signaling and alleviating chondrocyte senescence

Published: 2026-08-22

Journal: CELLULAR SIGNALLING

Impact Factor: 4.70

PMID: 42632530

SKU	PA2141-1
Application	Immunofluorescence
Sample	mouse cartilage tissue
Sample Processing Description	Freshly dissected bone samples were first fixed overnight in 4% paraformaldehyde (PFA), followed by decalcification for 3 days in 10% ethylenediaminetetraacetic acid (EDTA) solution, and then dehydrated for 1 day in 30% sucrose solution. The bone samples were subsequently embedded in optimal cutting temperature compound (OCT; Sakura, Cat. No. OCT-4583) and cryosectioned at a thickness of 10 μm using a Leica CryoJane cryostat.
Primary Antibody	Anti-Collagen II/COL2A1 Antibody Picoband®
Primary Incubation	1:100, overnight at 4 °C.
Secondary Antibody	donkey anti-rabbit Alexa Fluor 555 Secondary Antibody
Secondary Incubation	1:500, Incubate at room temperature for 1 hour
Other Reagents used	PBS with 10% horse serum, DAPI
Detection	fluorescence microscope (Leica)
Results Summary	I will purchase BosterBio products again and recommend them to my classmates and colleagues.

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Anti-Collagen II/COL2A1 Antibody Picoband®

Col2a1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Inhibition of PARP-1 attenuates osteoarthritis progression by suppressing NF-κB signaling and alleviating chondrocyte senescence

Stem Cell-Derived Extracellular Matrix Biofunctionalized Small Intestinal Submucosa for the Repair of Rotator Cuff Tears

Functionalized multilayer hydrogels loaded with HDAC4-modified superficial and middle-deep zone chondrocytes for enabling precise zonal repair of osteochondral defects

Iron Sequestration by Magnesium Prussian Blue to Ameliorate Osteoarthritis Through Restoration of HIF-1α Signaling and Ferroptosis Inhibition

Activation of SLIT2/ROBO1/LRP6 axis aggravates cartilage degradation via β-catenin signaling in TMJOA

Ovarian Matrisome Dynamics and αvβ3-Mediated Regulation in Early Follicular Development

Mechanically Preconditioned Biomimetic Gradient Scaffold for Tendon-to-Bone Regeneration in a Rabbit Rotator Cuff Tear Model

NsPEFs-enriched ADSCs-EVs alleviate osteoarthritis via RSPO3-mediated dual pro-chondrogenic and pro-M2 macrophage properties

Fibrous-layer resident Angptl7+ periosteal stem cells sense injury inflammation to orchestrate fracture repair

Telomere-to-telomere genome assembly of a male pig provides insight into population structure and selection for body stature

Targeting VGLL4 maintains extracellular matrix homeostasis and mitigates osteoarthritis in a preclinical model

Targeted Blockage of Pathological Extracellular Vesicles and Particles From Fibroblast-Like Synoviocytes for Osteoarthritis Relief: Proteomic Analysis and Cellular Effect

Growth factor independence 1 ameliorates osteoarthritis by inhibiting chondrocyte ferroptosis via inactivation of MAPK signaling pathway

SESN2 maintains cartilage homeostasis by SREBP1-mediated lipid metabolism during osteoarthritis progression

Procr+ chondroprogenitors sense mechanical stimuli to govern articular cartilage maintenance and regeneration

Tensile stress promotes the chondrogenic ability of condylar cartilage stem/progenitor cells in the temporomandibular joint via the Piezo1-Ca2+-Prkca pathway

Inhibiting the expression of GPR43 in macrophages can alleviate osteoarthritis by suppressing the M1 polarization and suppressing ROS production

Defective RuO2 Nanospheres Attenuate Osteoarthritis Progression via Suppressing the ROS/NLRP3/Caspase-1 Signaling Pathway

PTH Promotes Chondrogenesis of Fibrocartilage Stem Cells and Alleviates Temporomandibular Joint Osteoarthritis

Short-Term DMOG treatment rejuvenates senescent mesenchymal stem cells by enhancing mitochondrial function and mitophagy through the HIF-1α/BNIP3 pathway

Primary cilia in growth plates orchestrate long bone development

FAM20B-Catalyzed Glycosylation Regulates the Chondrogenic and Osteogenic Differentiation of the Embryonic Condyle by Controlling IHH Diffusion and Release

CD133+PD-L1+ cancer cells confer resistance to adoptively transferred engineered macrophage-based therapy in melanoma

1,25(OH)2D3 induces chondrocyte autophagy and reduces the loss of proteoglycans in osteoarthritis through inhibiting the NF-κB pathway

Calcium Fructoborate Improves Knee Osteoarthritis in Rats by Activating Hedgehog Signaling Through DDIT3

Hoxc10‐mediated ‘positional memory’ regulates cartilage formation subsequent to femoral heterotopic grafting

Amentoflavone maintaining extracellular matrix homeostasis and inhibiting subchondral bone loss in osteoarthritis by inhibiting ERK, JNK and NF-κB signaling pathways

Regulation of Ptbp1-controlled alternative splicing of pyruvate kinase muscle by Liver kinase b1 governs vascular smooth muscle cell plasticity in vivo

Promoting chondrogenesis by targeted delivery to the degenerating cartilage in early treatment of osteoarthritis

Collagen-based hydrogels induce stem cell chondrogenesis and hyaline cartilage regeneration: An in vivo study

Modified Si Miao Powder granules alleviates osteoarthritis progression by regulating M1/M2 polarization of macrophage through NF-κB signaling pathway

The Contribution of Meckel’s Cartilage-Derived Type II Collagen-Positive Cells to the Jawbone Development and Repair

Physically crosslinked poly(vinyl alcohol)-based hydrogels for cartilage tissue engineering

Spinosin ameliorates osteoarthritis through enhancing the Nrf2/HO-1 signaling pathway

Notch1 signaling regulates Sox9 and VEGFA expression and governs BMP2-induced endochondral ossification of mesenchymal stem cells

Bifunctional TRPV1 Targeted Magnetothermal Switch to Attenuate Osteoarthritis Progression

Near Infrared Responsive Gold Nanorods Attenuate Osteoarthritis Progression by Targeting TRPV1

Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis

XJB-5-131 protects chondrocytes from ferroptosis to alleviate osteoarthritis progression via restoring Pebp1 expression

Combined Transcriptomics and Metabolomics Identify Regulatory Mechanisms of Porcine Vertebral Chondrocyte Development In Vitro

WTAP-mediated m6A modification of FRZB triggers the inflammatory response via the Wnt signaling pathway in osteoarthritis

Identification and validation of FPR1, FPR2, IL17RA and TLR7 as immunogenic cell death related genes in osteoarthritis

Spatial and Temporal Expression of Ectodysplasin-A Signaling Pathway Members During Mandibular Condylar Development in Postnatal Mice

ADRM1/RPN13 attenuates cartilage extracellular matrix degradation via enhancing UCH37-mediated ALK5 deubiquitination

Identification of several inflammation-related genes based on bioinformatics and experiments

Primary cilia support cartilage regeneration after injury

Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments

Kartogenin potentially protects temporomandibular joints from collagenase-induced osteoarthritis via core binding factor β and runt-related transcription factor 1 binding - A rat model study

Inhibition of fibroblast activation protein ameliorates cartilage matrix degradation and osteoarthritis progression

Early diagnosis of heterotopic ossification with a NIR fluorescent probe by targeting type II collagen

Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice

Selenium-sensitive histone deacetylase 2 is required for forkhead box O3A and regulates extracellular matrix metabolism in cartilage

Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis

A sonication-induced silk-collagen hydrogel for functional cartilage regeneration

Improving the symptoms of post-traumatic osteoarthritis by α2-macroglobulin-rich serum

Nesfatin-1 exerts protective effects on acidosis-stimulated chondrocytes and rats with adjuvant-induced arthritis by inhibiting ASIC1a expression

Harnessing Bifunctional Ferritin with Kartogenin Loading for Mesenchymal Stem Cell Capture and Enhancing Chondrogenesis in Cartilage Regeneration

Single-cell transcriptomics of LepR-positive skeletal cells reveals heterogeneous stress-dependent stem and progenitor pools

3D printed PCL/GelMA biphasic scaffold boosts cartilage regeneration using co-culture of mesenchymal stem cells and chondrocytes: In vivo study

Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose Hydrogel

A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real-Time Monitoring of Cells

Elevated lymphotoxin-α (TNFβ) is associated with intervertebral disc degeneration

Inhibitory effect on the apoptosis of disc nucleus pulposus cells in rats by silencing COL9A3 gene to mediate MAPK signaling pathway: a study on the function and mechanism

Chondroitin sulfate modified 3D porous electrospun nanofiber scaffolds promote cartilage regeneration

Involvement of Bmal1 and circadian clock signaling in chondrogenic differentiation of ATDC5 cells by fluoride

Neferine Inhibits Expression of Inflammatory Mediators and Matrix Degrading Enzymes in IL-1β-Treated Rat Chondrocytes via Suppressing MAPK and NF-κB Signaling Pathways

A CRISPR-engineered swine model of COL2A1 deficiency recapitulates altered early skeletal developmental defects in humans